I. Lazarkevich, S. Engibarov, Simona Mitova, E. Vacheva, Steliyana Popova, Nikola Stanchev, Rumyana T. Eneva, Y. Gocheva, Ivanka Boyadzhieva, M. Gerginova
{"title":"基于 16S rRNA 基因测序的保加利亚西部低山地区五种综合蜥蜴粪便微生物群的鉴定和比较分析","authors":"I. Lazarkevich, S. Engibarov, Simona Mitova, E. Vacheva, Steliyana Popova, Nikola Stanchev, Rumyana T. Eneva, Y. Gocheva, Ivanka Boyadzhieva, M. Gerginova","doi":"10.3390/applmicrobiol4010013","DOIUrl":null,"url":null,"abstract":"Studies on the gut microbiome of free-living reptiles in Europe are generally fragmentary and still missing in Bulgaria. We aimed to identify and compare the fecal microbiota profiles of five syntopic lizard species from three families: the European green lizard (Lacerta viridis), the common wall lizard (Podarcis muralis), the meadow lizard (Darevskia praticola) (Lacertidae), the European snake-eyed skink (Ablepharus kitaibelii) (Scincidae), and the European slow worm (Anguis fragilis) (Anguidae), which coinhabit a low mountainous area in the western part of the country. A high-throughput sequencing of the hypervariable V3-V4 region of the 16S rRNA gene, performed on the Illumina HiSeq2500 platform, was used. The core microbiota of lizard hosts seems to be species-specific. A dynamic phyla proportion between hosts was found. The richest alpha diversity was observed in D. praticola, and the lowest alpha diversity was observed in P. muralis and A. fragilis. Within the three lacertids, the microbiota of D. praticola and L. viridis were more closely related to each other than they were to those of P. muralis. Sharing a largely common trophic resource (all species except A. fragilis are mainly insectivorous) was not an indication of similarity in their gut microbial communities.","PeriodicalId":8080,"journal":{"name":"Applied microbiology","volume":"66 13","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"16S rRNA Gene Sequencing-Based Identification and Comparative Analysis of the Fecal Microbiota of Five Syntopic Lizard Species from a Low-Mountain Area in Western Bulgaria\",\"authors\":\"I. Lazarkevich, S. Engibarov, Simona Mitova, E. Vacheva, Steliyana Popova, Nikola Stanchev, Rumyana T. Eneva, Y. Gocheva, Ivanka Boyadzhieva, M. Gerginova\",\"doi\":\"10.3390/applmicrobiol4010013\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Studies on the gut microbiome of free-living reptiles in Europe are generally fragmentary and still missing in Bulgaria. We aimed to identify and compare the fecal microbiota profiles of five syntopic lizard species from three families: the European green lizard (Lacerta viridis), the common wall lizard (Podarcis muralis), the meadow lizard (Darevskia praticola) (Lacertidae), the European snake-eyed skink (Ablepharus kitaibelii) (Scincidae), and the European slow worm (Anguis fragilis) (Anguidae), which coinhabit a low mountainous area in the western part of the country. A high-throughput sequencing of the hypervariable V3-V4 region of the 16S rRNA gene, performed on the Illumina HiSeq2500 platform, was used. The core microbiota of lizard hosts seems to be species-specific. A dynamic phyla proportion between hosts was found. The richest alpha diversity was observed in D. praticola, and the lowest alpha diversity was observed in P. muralis and A. fragilis. Within the three lacertids, the microbiota of D. praticola and L. viridis were more closely related to each other than they were to those of P. muralis. Sharing a largely common trophic resource (all species except A. fragilis are mainly insectivorous) was not an indication of similarity in their gut microbial communities.\",\"PeriodicalId\":8080,\"journal\":{\"name\":\"Applied microbiology\",\"volume\":\"66 13\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-01-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/applmicrobiol4010013\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/applmicrobiol4010013","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
对欧洲自由生活的爬行动物肠道微生物组的研究普遍比较零散,在保加利亚仍然缺失。我们的目的是鉴定和比较来自三个科的五个同科蜥蜴物种的粪便微生物群特征:欧洲绿蜥(Lacerta viridis)、普通壁蜥(Podarcis muralis)、草甸蜥(Darevskia praticola)(Lacertidae)、欧洲蛇眼鼬(Ablepharus kitaibelii)(Scincidae)和欧洲慢蠕虫(Anguis fragilis)(Anguidae),它们共同生活在保加利亚西部的低山区。在 Illumina HiSeq2500 平台上对 16S rRNA 基因的 V3-V4 超变区进行了高通量测序。蜥蜴宿主的核心微生物群似乎具有物种特异性。研究发现,宿主之间的系统比例是动态的。在 D. praticola 中观察到最丰富的 alpha 多样性,而在 P. muralis 和 A. fragilis 中观察到最低的 alpha 多样性。在三种漆虫中,D. praticola 和 L. viridis 的微生物群之间的关系比 P. muralis 的微生物群之间的关系更为密切。在很大程度上共享共同的营养资源(除A. fragilis外,所有物种都以食虫为主)并不表明它们的肠道微生物群落具有相似性。
16S rRNA Gene Sequencing-Based Identification and Comparative Analysis of the Fecal Microbiota of Five Syntopic Lizard Species from a Low-Mountain Area in Western Bulgaria
Studies on the gut microbiome of free-living reptiles in Europe are generally fragmentary and still missing in Bulgaria. We aimed to identify and compare the fecal microbiota profiles of five syntopic lizard species from three families: the European green lizard (Lacerta viridis), the common wall lizard (Podarcis muralis), the meadow lizard (Darevskia praticola) (Lacertidae), the European snake-eyed skink (Ablepharus kitaibelii) (Scincidae), and the European slow worm (Anguis fragilis) (Anguidae), which coinhabit a low mountainous area in the western part of the country. A high-throughput sequencing of the hypervariable V3-V4 region of the 16S rRNA gene, performed on the Illumina HiSeq2500 platform, was used. The core microbiota of lizard hosts seems to be species-specific. A dynamic phyla proportion between hosts was found. The richest alpha diversity was observed in D. praticola, and the lowest alpha diversity was observed in P. muralis and A. fragilis. Within the three lacertids, the microbiota of D. praticola and L. viridis were more closely related to each other than they were to those of P. muralis. Sharing a largely common trophic resource (all species except A. fragilis are mainly insectivorous) was not an indication of similarity in their gut microbial communities.