SP142 和 SP263 抗体克隆检测到的 PD-L1 状态与胃癌临床和形态特征之间的关系

T. N. Sotnikova, T. V. Polushkina, D. Kalinin, N. Danilova
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The positive PD-L1 status detected by clones SP263 and SP142 is significantly associated with type 2 macroscopic form according to the R. Bormann classification (p=0,003/p=0,003), tubular morphological type according to the WHO 5th edition, 2019 classification (p=0,001/p=0,018), intermediate morphological type according to the classification of P. Lauren (p=0,027/p=0,005) and the absence of signet ring cells (p=0,001/p=0,010). Staining with both clones used was not associated with the gender and age of patients, the size and localization of the tumor, the degree of its differentiation, the presence or absence of emboli in the lumen of blood and lymph vessels, the presence of affected lymph nodes and distant metastases, as well as the clinical stage (p>0,05). 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引用次数: 0

摘要

目的是研究胃癌 SP142 和 SP263 克隆的 PD-L1 状态与其临床和形态学参数的关系。材料与方法从 131 名确诊为胃癌的患者身上获取手术材料。127 例患者的 PD-L1 SP263 抗体被染色,126 例患者的 PD-L1 SP142 抗体被染色。根据一抗生产商推荐的方案,使用 Ventana BenchMark Ultra 设备(美国罗氏 Ventana 公司)进行 PD-L1 反应。通过统计分析将免疫组化染色结果与胃癌的临床和形态特征进行比较。结果SP263和SP142克隆检测到的PD-L1阳性状态与R. Bormann分类法的2型大体形态(p=0,003/p=0,003)、2019年WHO第5版分类法的管状形态(p=0,001/p=0,018)、P. Lauren分类法的中间形态(p=0,027/p=0,005)和无标志环细胞(p=0,001/p=0,010)显著相关。两种克隆的染色与患者的性别和年龄、肿瘤的大小和位置、分化程度、血管和淋巴管腔内有无栓子、有无受累淋巴结和远处转移以及临床分期无关(p>0.05)。在 SP263/SP142 克隆研究中,PD-L1 阳性病例比例最高的是根据 2019 年世界卫生组织第 5 版分类发现的管型肿瘤(75.6%/84.6%)、根据 P. Lauren 分类发现的中间型肿瘤(60.0%/68.0%)、根据 R. Bormann 分类发现的 2 型肿瘤(65.4%/68.0%)以及无标志环细胞的肿瘤(70.7%/76.9%)。结论具有统计学意义的胃癌临床和形态学参数与 PD-L1 克隆 SP263 和 SP142 的表达之间存在可靠的关系。Bormann (p=0,003/p=0,003),根据2019年WHO第5版的分类,为管状形态(p=0,001/p=0,018),根据P. Lauren分类,为中间型(p=0,027/p=0,005),无标志环细胞(p=0,001/p=0,010)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Association Between PD-L1 status Detected by SP142 and SP263 Antibody Clones and Clinical and Morphological Features of Gastric Cancer Factors
The aim is to study the relationship of PD-L1 status of SP142 and SP263 clones in gastric cancer with its clinical and morphological parameters. Material and methods. Surgical material obtained from 131 patients with a confirmed diagnosis of stomach cancer. Antibodies to PD-L1 SP263 were stained 127 cases, antibodies to PD-L1 SP142 – 126 cases. Reactions with PD-L1 were performed using the Ventana BenchMark Ultra device (Roche Ventana, USA) according to the protocols recommended by the manufacturer of primary antibodies. The results of immunohistochemical staining were compared with the clinical and morphological characteristics of gastric cancer using statistical analysis. Results. The positive PD-L1 status detected by clones SP263 and SP142 is significantly associated with type 2 macroscopic form according to the R. Bormann classification (p=0,003/p=0,003), tubular morphological type according to the WHO 5th edition, 2019 classification (p=0,001/p=0,018), intermediate morphological type according to the classification of P. Lauren (p=0,027/p=0,005) and the absence of signet ring cells (p=0,001/p=0,010). Staining with both clones used was not associated with the gender and age of patients, the size and localization of the tumor, the degree of its differentiation, the presence or absence of emboli in the lumen of blood and lymph vessels, the presence of affected lymph nodes and distant metastases, as well as the clinical stage (p>0,05). The largest proportion of PD-L1 positive cases in the study of SP263/SP142 clones was found in tubular type tumors (75,6%/84,6%) according to the WHO 5th edition, 2019 classification, intermediate type (60,0%/68,0%) according to P. Lauren, type 2 (65,4%/68,0%) according to R. Bormann and in the absence of signet ring cells (70,7%/76,9%). Conclusion. Statistically significant clinical and morphological parameters of gastric cancer, where a reliable relationship between them and the expression of PD-L1 clones SP263 and SP142 was revealed, are: type 2 macroscopic form according to R. Bormann (p=0,003/p=0,003), tubular morphological type according to the classification of WHO 5th edition, 2019) (p=0,001/p=0,018), intermediate type according to P. Lauren (p=0,027/p=0,005) and the absence of signet ring cells (p=0,001/p=0,010).
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