利用乳酸乳球菌以类菌体颗粒形式生产猪德他克隆病毒植物重组疫苗

IF 2.2 4区 生物学 Q2 PLANT SCIENCES
Gyeongryul Ryu, Gyeongik Ahn, Joon-Yung Cha, Kris John Silvano, Changhee Lee, Kyeong-Ryeol Lee, Inhwan Hwang, Woe-Yeon Kim, Min Gab Kim
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引用次数: 0

摘要

猪三角冠状病毒(PDCoV)是一种肠道致病性猪冠状病毒,可引起哺乳仔猪和幼猪严重腹泻,导致死亡。母猪向仔猪传递母源中和抗体是保护仔猪免受 PDCoV 感染的关键。因此,开发有效的疫苗需要高免疫原性的抗原。在此,我们利用乳酸乳球菌(Lactococcus lactis)以类菌颗粒(BLPs)的形式生产了基于植物的 PDCoV 重组蛋白疫苗,以增强其抗原性。KNU16-07 菌株的尖峰蛋白 S1 结构域被用作抗原。抗原与赖氨酸基序(LysM)和冠状蛋白 1 的卷曲结构域(ccCor1)融合,用于乳球菌表面展示。这种融合策略促进了表达蛋白的分离,并增加了抗原性。重组蛋白在烟草中表达并纯化为 BLPs。为了确定 PDCoV S1 包被的 BLPs 能否在没有佐剂的情况下进行免疫,从注射了抗原的小鼠身上获取了血浆样本。总之,由 N. benthamiana 产生的 PDCoV S1 结构域作为一种潜在的抗原,能很好地产生抗体,可被视为一种有效的疫苗,用于仔猪对 PDCoV 的被动免疫。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Production of Plant-Based Recombinant Vaccine Against Porcine Deltacoronavirus in the Form of Bacteria-Like Particles Using Lactococcus lactis

Production of Plant-Based Recombinant Vaccine Against Porcine Deltacoronavirus in the Form of Bacteria-Like Particles Using Lactococcus lactis

Porcine deltacoronavirus (PDCoV) is an enteropathogenic swine coronavirus that causes severe diarrheal disease in suckling piglets and young pigs, leading to death. Transfer of maternal neutralizing antibodies from sows to piglets is critical to protecting piglets from PDCoV. Therefore, highly immunogenic antigens are required for the development of effective vaccine. Here, we produced plant-based recombinant protein vaccine against PDCoV in the form of bacteria-like particles (BLPs) using Lactococcus lactis to enhance the antigenicity. The S1 domain of spike protein from KNU16-07 strain was used as an antigen. The antigen was fused with lysine motifs (LysM) and coiled domains of coronin 1 (ccCor1) for L. lactis surface display. This fusion strategy facilitated the separation of the expressed proteins and increased antigenicity. The recombinant proteins were expressed in Nicotiana benthamiana and purified as BLPs. To determine whether PDCoV S1-coated BLPs could be immunized in the absence of adjuvants, plasma samples were obtained from mice injected with antigen. In conclusion, the PDCoV S1 domain generated from N. benthamiana excellently produced antibodies as a potential antigen and can be considered as an effective vaccine for passive immunization of piglets against PDCoV.

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来源期刊
Journal of Plant Biology
Journal of Plant Biology 生物-植物科学
CiteScore
5.40
自引率
3.40%
发文量
29
审稿时长
1 months
期刊介绍: Journal of Plant Biology, an official publication of the Botanical Society of Korea, is an international journal devoted to basic researches in biochemistry, cellular biology, development, ecology, genetics, molecular biology, physiology, and systematics of plants. The Journal publishes the following categories of paper: Original articles -- For publication in Journal of Plant Biology the manuscript must provide a significant new contribution to our understanding of plants. All areas of plant biology are welcome. No limit on the length, but a concise presentation is encouraged. Reviews -- Invited by the EiC. Brief Communications -- Concise but independent report representing significant contribution to plant science. The Botanical Society of Korea was founded on November 30, 1957 to promote studies, disseminate and exchange information on the field of plant biology. The first issue of The Korean Journal of Botany, the official publication of the society, was published on April 1, 1958. It was published twice a year, but quarterly from 5th volume in 1962. In 1994, it was renamed to Journal of Plant Biology and published in English since 1996. The journal entered its 50th year of publication in 2007.
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