明确阐明锐尖叶蝉接种Xylella fastidiosa的机制

IF 5.6 1区 农林科学 Q1 ENTOMOLOGY
Daniele Cornara, Isabel Bojanini, Alberto Fereres, Rodrig.P.P. Almeida
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引用次数: 0

摘要

Xylella fastidiosa 是一种病媒传播的木质部局限性细菌,对农业和经济具有重要意义,但这种病原体是如何通过昆虫载体接种到植物中的仍有待证实。最近对草地唾液蝽(Phaenus spumarius)进行的 DC-EPG(电穿透图)研究初步证实,细菌接种与一种称为 Xe 的非定型 "尖峰波形 "有关;这种行为发生在花柱最初接触木质部血管的最初几分钟内。在这里,我们利用 Graphocephala atropunctata-grapevine-X. fastidiosa 病理系统,确证了直流电谱波形 Xe 代表了与 X. fastidiosa 接种到寄主植物并成功造成系统性持续病原体感染相关的载体探测行为。我们的工作支持了之前的假设,即 Xe 代表了木质部汁液-细菌细胞混合物的排出行为,当木质部前瓣膜扇动时,木质部汁液-细菌细胞混合物被塌陷的木质部前瓣膜推动,可能是为了清除木质部前瓣膜及其化学感受器的阻塞。由于瓣膜的扇动而不是封闭食道,因此可以将汁液和细菌细胞的混合物向外输送到植物体内。在前肠中携带 X. fastidiosa 细胞的媒介中,Xe 的接种行为更为频繁,这与昆虫体内的细菌数量无关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Definitive elucidation of the inoculation mechanism of Xylella fastidiosa by sharpshooter leafhoppers
Xylella fastidiosa is a vector-borne xylem-limited bacterium of agricultural and economic importance, yet it remains to be demonstrated how this pathogen is inoculated into plants by insect vectors. Recent DC-EPG (Electrical Penetration Graph) research with the meadow spittlebug, Philaenus spumarius tentatively correlated bacterial inoculation with a non-stereotypical “spiking waveform” termed Xe; this behavior occurred within the first minutes from initial stylets contact with a xylem vessel. Here, using the Graphocephala atropunctata-grapevine-X. fastidiosa pathosystem, we conclusively demonstrate that the DC-EPG waveform Xe represents the vector probing behavior correlated with X. fastidiosa inoculation into a host plant, and successful systemic persistent pathogen infection. Our work supports the previous hypothesis that Xe represents an egestion behavior, with a mixture of xylem sap-bacterial cells propelled by the collapse of the cibarial diaphragm while the precibarial valve is fluttering, potentially to remove obstructions from the precibarium and its chemosensilla. The outward flow delivering the mixture sap-bacterial cells into the plant is permitted by the valve that flutters instead of sealing the food canal. The inoculation behavior Xe is more frequent in vectors harboring X. fastidiosa cells within the foregut, independent of the bacterial population size in insects.
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来源期刊
Entomologia Generalis
Entomologia Generalis 生物-昆虫学
CiteScore
7.10
自引率
18.80%
发文量
72
审稿时长
>12 weeks
期刊介绍: Its scope covers all aspects of basic and applied research dealing with insects and more broadly with arthropods inhabiting wild, agricultural and/or urban habitats. The journal also considers research integrating various disciplines and issues within the broad field of entomology and ecology. Entomologia Generalis publishes high quality research articles on advances in knowledge on the ecology and biology of arthropods, as well as on their importance for key ecosystems services, e.g. as biological control and pollination. The journal devotes special attention to contributions providing significant advances (i) on the fundamental knowledge and on sustainable control strategies of arthropod pests (including of stored products) and vectors of diseases, (ii) on the biology and ecology of beneficial arthropods, (iii) on the spread and impact of invasive pests, and (iv) on potential side effects of pest management methods. Entomologia Generalis welcomes review articles on significant developments in the field of entomology. These are usually invited by the editorial board, but proposals may be sent to the Editor-in-Chief for preliminary assessment by the editorial board before formal submission to the journal. The journal also considers comments on papers published in Entomologia Generalis, as well as short notes on topics that are of broader interest.
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