Joerg Ermann, Micah Lefton, Kevin Wei, Maria Gutierrez-Arcelus
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引用次数: 0
摘要
综述的目的:悬浮或组织背景下的单细胞图谱分析是一个快速发展的领域。本综述旨在概述最近的进展和新出现的趋势,并特别关注脊柱关节炎的研究:基于测序的单细胞定量 RNA、蛋白质或表观遗传修饰方法的引入极大地推动了以发现为导向的研究。荧光流式细胞术、质控细胞术和基于图像的细胞术不断发展。空间转录组学和成像质量细胞仪将高维分析扩展到了组织中的细胞。在脊柱关节炎中的应用包括对细胞进行索引和功能表征、发现与疾病相关的细胞状态以及识别与治疗反应相关的特征。单细胞 TCR-seq 为脊柱关节炎中 CD8+ T 细胞的克隆扩增提供了证据。单细胞分析方法在脊柱关节炎研究中的应用仍处于早期阶段。面临的挑战包括成本高昂和病变组织样本有限。要充分利用迅速发展的技术能力,大规模的合作势在必行。
Understanding Spondyloarthritis Pathogenesis: The Promise of Single-Cell Profiling.
Purpose of review: Single-cell profiling, either in suspension or within the tissue context, is a rapidly evolving field. The purpose of this review is to outline recent advancements and emerging trends with a specific focus on studies in spondyloarthritis.
Recent findings: The introduction of sequencing-based approaches for the quantification of RNA, protein, or epigenetic modifications at single-cell resolution has provided a major boost to discovery-driven research. Fluorescent flow cytometry, mass cytometry, and image-based cytometry continue to evolve. Spatial transcriptomics and imaging mass cytometry have extended high-dimensional analysis to cells in tissues. Applications in spondyloarthritis include the indexing and functional characterization of cells, discovery of disease-associated cell states, and identification of signatures associated with therapeutic responses. Single-cell TCR-seq has provided evidence for clonal expansion of CD8+ T cells in spondyloarthritis. The use of single-cell profiling approaches in spondyloarthritis research is still in its early stages. Challenges include high cost and limited availability of diseased tissue samples. To harness the full potential of the rapidly expanding technical capabilities, large-scale collaborative efforts are imperative.
期刊介绍:
This journal aims to review the most important, recently published research in the field of rheumatology. By providing clear, insightful, balanced contributions by international experts, the journal intends to serve all those involved in the care and prevention of rheumatologic conditions.
We accomplish this aim by appointing international authorities to serve as Section Editors in key subject areas such as the many forms of arthritis, osteoporosis and metabolic bone disease, and systemic lupus erythematosus. Section Editors, in turn, select topics for which leading experts contribute comprehensive review articles that emphasize new developments and recently published papers of major importance, highlighted by annotated reference lists. An international Editorial Board reviews the annual table of contents, suggests articles of special interest to their country/region, and ensures that topics are current and include emerging research. Commentaries from well-known figures in the field are also occasionally provided.