揭示高粱品种在变异霉素诱导的内质网胁迫下不同代谢物的调节。

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Francisco Lucas Pacheco Cavalcante, Sávio Justino da Silva, Lineker de Sousa Lopes, Stelamaris de Oliveira Paula-Marinho, Maria Izabel Florindo Guedes, Enéas Gomes-Filho, Humberto Henrique de Carvalho
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引用次数: 0

摘要

植物通过内质网(ER)途径来抵御胁迫,但ER对植物耐受性的帮助仍有待探索。因此,我们选择了对非生物胁迫比较敏感和耐受性较强的高粱品种,以测试它们是否对ER胁迫具有一定程度的耐受性。因此,本研究评估了新月形浓度的妥尼霉素(TM µg mL-1):对照(0)、较低浓度(0.5)、温和浓度(1.5)和较高浓度(2.5)对高粱幼苗 CSF18 和 CSF20 初期生长的影响。从轻度到较高的 TM,ER 胁迫促进了生长和新陈代谢的降低,主要是 CSF18。最低的 TM 增加了 SbBiP 和 SbPDI 合子以及 SbbZIP60 和 SbbIRE1 基因的表达,而轻度和较高的 TM 则降低了其表达。然而,CSF20 的 SbBiP 和 SbbIRE1 转录本水平更高。这证实了 CSF18 嫩芽在所有 TM 处理中的不同代谢特征,以及 CSF18 根系在温和与较高 TM 处理中的相似特征。相反,CSF20 的芽和根的 TM 图谱有所重叠,尽管在低 TM 处理下并不完全重叠。此外,ER胁迫诱导CSF20中碳水化合物(芽中的二羟基丙酮,根中的纤维生物糖、麦芽糖、核糖和蔗糖)和有机酸(芽中的丙酮酸,根中的丁酸和琥珀酸)的增加,与CSF18相比,CSF20表现出更高的ER胁迫耐受性,而根是受影响最大的植物组织。因此,我们的研究提供了新的见解,可能有助于了解高粱的耐受性以及ER干扰对胁迫适应和耐受性工程的重要贡献。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unveiling a differential metabolite modulation of sorghum varieties under increasing tunicamycin-induced endoplasmic reticulum stress.

Plants trigger endoplasmic reticulum (ER) pathways to survive stresses, but the assistance of ER in plant tolerance still needs to be explored. Thus, we selected sensitive and tolerant contrasting abiotic stress sorghum varieties to test if they present a degree of tolerance to ER stress. Accordingly, this work evaluated crescent concentrations of tunicamycin (TM µg mL-1): control (0), lower (0.5), mild (1.5), and higher (2.5) on the initial establishment of sorghum seedlings CSF18 and CSF20. ER stress promoted growth and metabolism reductions, mainly in CSF18, from mild to higher TM. The lowest TM increased SbBiP and SbPDI chaperones, as well as SbbZIP60, and SbbIRE1 gene expressions, but mild and higher TM decreased it. However, CSF20 exhibited higher levels of SbBiP and SbbIRE1 transcripts. It corroborated different metabolic profiles among all TM treatments in CSF18 shoots and similarities between profiles of mild and higher TM in CSF18 roots. Conversely, TM profiles of both shoots and roots of CSF20 overlapped, although it was not complete under low TM treatment. Furthermore, ER stress induced an increase of carbohydrates (dihydroxyacetone in shoots, and cellobiose, maltose, ribose, and sucrose in roots), and organic acids (pyruvic acid in shoots, and butyric and succinic acids in roots) in CSF20, which exhibited a higher degree of ER stress tolerance compared to CSF18 with the root being the most affected plant tissue. Thus, our study provides new insights that may help to understand sorghum tolerance and the ER disturbance as significant contributor for stress adaptation and tolerance engineering.

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来源期刊
Cell Stress & Chaperones
Cell Stress & Chaperones 生物-细胞生物学
CiteScore
7.60
自引率
2.60%
发文量
59
审稿时长
6-12 weeks
期刊介绍: Cell Stress and Chaperones is an integrative journal that bridges the gap between laboratory model systems and natural populations. The journal captures the eclectic spirit of the cellular stress response field in a single, concentrated source of current information. Major emphasis is placed on the effects of climate change on individual species in the natural environment and their capacity to adapt. This emphasis expands our focus on stress biology and medicine by linking climate change effects to research on cellular stress responses of animals, micro-organisms and plants.
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