SIRT6 和 NMNAT2 的下调与增殖性糖尿病视网膜病变有关。

IF 1.8 3区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Vision Pub Date : 2023-10-02 eCollection Date: 2023-01-01

Hui Chen, Xiongze Zhang, Nanying Liao, Yuying Ji, Lan Mi, Yuhong Gan, Yongyue Su, Feng Wen

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引用次数: 0

摘要

目的：测定糖尿病视网膜病变（DR）中 SIRT6 和 NMNAT2 的表达水平：我们采集了 77 名 2 型糖尿病患者的外周血单核细胞（PBMC）和玻璃体样本：方法：我们采集了 77 名 2 型糖尿病患者的外周血单核细胞（PBMC）和玻璃体样本，其中 52 人患有 DR，25 人未患 DR，另外还有 27 名健康对照者。研究人员采用 Western 印迹分析和 qRT-PCR 技术评估了患者 PBMCs 中 SIRT6 和 NMNAT2 的表达情况。玻璃体液中的 IL-1β、IL-6 和 TNF-α 水平是通过 ELISA 法测定的。免疫组化法检测增殖性DR（PDR）和对照组中SIRT6和NMNAT2的表达：结果：SIRT6和NMNAT2的表达在DR患者中明显下调，这与IL-1β、IL-6和TNF-α的表达增加呈负相关。此外，我们还观察到SIRT6和NMNAT2在PDR患者纤维血管膜中的表达下降：结论：SIRT6和NMNAT2在PDR患者中的表达下调揭示了一种潜在的致病关联；更多的扩展研究可以验证它们是潜在的治疗靶点。

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Downregulation of SIRT6 and NMNAT2 is associated with proliferative diabetic retinopathy.

Purpose: To determine the expression levels of SIRT6 and NMNAT2 in diabetic retinopathy (DR).

Methods: We obtained peripheral blood mononuclear cells (PBMCs) and vitreous samples from 77 patients with type 2 diabetes mellitus: 52 with DR and 25 without DR, and 27 healthy control subjects. Western blot analysis and qRT-PCR were performed to evaluate the expression of SIRT6 and NMNAT2 in their PBMCs. The levels of IL-1β, IL-6, and TNF-α in the vitreous fluid were determined by ELISA. Immunohistochemistry was performed to detect the expression of SIRT6 and NMNAT2 in proliferative DR (PDR) and the control subjects.

Results: The expression of SIRT6 and NMNAT2 was markedly downregulated in DR patients, which was negatively correlated with the increased expression of IL-1β, IL-6 and TNF-α. Additionally, we observed decreased expression of SIRT6 and NMNAT2 in the fibrovascular membranes of PDR patients.

Conclusions: The downregulated expression of SIRT6 and NMNAT2 in PDR patients reveals a potential pathogenic association; more extended studies could verify them as potential therapeutic targets.

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来源期刊

Molecular Vision 生物-生化与分子生物学

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Molecular Vision is a peer-reviewed journal dedicated to the dissemination of research results in molecular biology, cell biology, and the genetics of the visual system (ocular and cortical). Molecular Vision publishes articles presenting original research that has not previously been published and comprehensive articles reviewing the current status of a particular field or topic. Submissions to Molecular Vision are subjected to rigorous peer review. Molecular Vision does NOT publish preprints. For authors, Molecular Vision provides a rapid means of communicating important results. Access to Molecular Vision is free and unrestricted, allowing the widest possible audience for your article. Digital publishing allows you to use color images freely (and without fees). Additionally, you may publish animations, sounds, or other supplementary information that clarifies or supports your article. Each of the authors of an article may also list an electronic mail address (which will be updated upon request) to give interested readers easy access to authors.