R2R3-MYB转录因子是否会通过调节液泡pH值来影响红掌佛焰苞的颜色变化?

IF 0.4 4区 综合性期刊 Q4 MULTIDISCIPLINARY SCIENCES
N.V.T. Jayaprada, S. Fukai, S. Geekiyanage
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引用次数: 0

摘要

红掌是世界上很受欢迎的切花,有红色、粉色、珊瑚色、白色、绿色或棕色等花色。商业市场对新花色的需求持续不断。植物的花色主要由花青素决定。了解花青素变异及其他影响红掌佛焰苞颜色的因素对基因工程方法非常重要。因此,我们的目标是评估影响部分市售切花红掌颜色变化的因素,并确定相关的调控网络和转录因子(TFs)。为此,我们选择了 19 个商业切花红掌栽培品种。记录了花被表面的颜色、花青素位置、花青素类型和液泡 pH 值。进行了花青素相关基因网络模型的生成和分析。CIELAB 色度计程序显示了所选 19 个栽培品种在颜色类型、颜色强度、色度和色调角度方面的颜色差异。花青素的位置仅限于叶肉细胞和表皮细胞。在测试的红掌栽培品种中检测到的花青素是主要的花青素。色素提取物中的 pH 值梯度显示在 4.6 至 4.94 之间。根据基因网络模型,花青素生物合成和运输的基因途径与液泡 pH/H+ 泵的基因途径相关。三条途径受 R2R3-MYB 转录因子调控。虽然花青素是所有测试品种中唯一的色素,但 R2R3-MYB 调控的 V-H+ 合成酶调节的 pH 值不同,这可能是造成颜色差异大的原因,此外还有花青素的类型和位置。我们的研究结果表明,R2R3-MYB 转录因子基因可用于蓝掌遗传工程中维持理想的液泡 pH 值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Does an R2R3-MYB transcription factor affect anthurium spathe colour variation via regulation of vacuolar pH?
The anthurium is a popular cut flower worldwide having red, pink, coral, white, green, or brown spathes. There is a constant demand for new flower colours in the commercial market. Flower colour in plants is mainly determined by anthocyanins. Understanding anthocyanin variation and other factors affecting anthurium spathe colour is important for genetic engineering approaches. Therefore, our objectives were to assess the factors affecting colour variation of selected commercially available cut flower anthuriums and to determine the associated regulatory networks and transcription factors (TFs). Nineteen commercial cut flower anthurium cultivars were selected for this purpose. The colour of the spathe surface, anthocyanin location, anthocyanidin type and vacuolar pH were recorded. Anthocyanin associated Gene Network Model generation and analysis were carried out. The CIELAB colourimeter procedure indicated the colour variation among the selected 19 cultivars in terms of colour type, colour intensity, chroma, and hue angle. The location of anthocyanin was limited to mesophyll and epidermal cells. Cyanidin was detected in tested anthurium cultivars as the main anthocyanidin. The pH gradient in pigment extracts indicated a variation with a range of 4.6 to 4.94. The gene pathways of anthocyanin biosynthesis and transport were associated with that of the vacuolar pH/H+ pump according to Gene Network Model. Three pathways were regulated by an R2R3-MYB transcription factor. Although, cyanidin was the only pigment in all the tested cultivars, different pH levels by R2R3-MYB regulated V-H+ synthase was suggested to be the cause of the high colour variation in addition to the anthocyanidin type and location. Our results indicate the application of R2R3-MYB transcription factor genes for desirable vacuolar pH maintenance in genetic engineering of the blue anthurium in the future.
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来源期刊
CiteScore
0.90
自引率
0.00%
发文量
57
审稿时长
>12 weeks
期刊介绍: The Journal of National Science Foundation of Sri Lanka (JNSF) publishes the results of research in Science and Technology. The journal is released four times a year, in March, June, September and December. This journal contains Research Articles, Reviews, Research Communications and Correspondences. Manuscripts submitted to the journal are accepted on the understanding that they will be reviewed prior to acceptance and that they have not been submitted for publication elsewhere.
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