扩增子测序确定了一种假定的病原体--相叶巨霉菌,该病原体会导致坦桑尼亚和乌干达种植的非洲茄子(Solanum aethiopicum)枯萎病

IF 3.5 Q1 AGRONOMY
Xiangming Xu, R. Minja, E. Kizito, F. Dinssa, G. Deakin, Pamela Nahamya Kabod, Asheri Kalala, Eliciana Kweka, O. Mbwambo, Deusdedith Mbanzibwa, Hamza Msangi, Mildred Julian Nakanwagi, T. Passey, Stuart Sentance, G. Sseremba, E. Stavridou, Gerard J. Bishop
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引用次数: 0

摘要

非洲茄子(Solanum aethiopicum L.)是坦桑尼亚和乌干达最常见的传统蔬菜之一,但由多种病原体引起的枯萎病严重影响了其产量。为了鉴定导致非洲茄子枯萎病的假定病原体,我们在坦桑尼亚 Gilo 组和乌干达 Shum 组的多块田地里采集了许多相邻的病株和健康植株的茎和根样本。通过对病株和健康组织进行扩增子测序,我们确定了导致枯萎病症状的假定病原体。在乌干达,S. aethiopicum 的枯萎病很可能是由细菌病原体 Ralstonia solanacearum 引起的,而在坦桑尼亚,枯萎病很可能是由感染根部的真菌病原体 Macrophomina phaseolina 引起的。在坦桑尼亚,茎部受到 Fusarium solani 的感染也可能导致枯萎病症状。在受控条件下进行的进一步人工接种证实,M. phaseolina 可导致 S. aethiopcium 基因型出现典型的枯萎症状。作物枯萎病不同病原的发现表明,在开发和实施有效的控制方法之前,需要对枯萎病进行具体的病因分析。还需要进一步研究,以确认结果,并针对具体的枯萎病病原体制定适当的管理措施。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Amplicon sequencing identified a putative pathogen, Macrophomina phaseolina, causing wilt in African eggplant (Solanum aethiopicum) grown in Tanzania and Uganda
African eggplant (Solanum aethiopicum L.) is one of the most common traditional vegetables in Tanzania and Uganda, but its productivity is severely affected by wilt diseases caused by a number of pathogens. Plant stem and root samples were collected in several fields from many neighboring diseased and healthy plants of the Gilo group in Tanzania and from the Shum group in Uganda to identify putative pathogens causing wilt on African eggplants. Through amplicon sequencing of sampled diseased and healthy tissues, we identified putative causal pathogens for the wilt symptoms. Wilting of S. aethiopicum in Uganda is most likely caused by the bacterial pathogen Ralstonia solanacearum whereas, in Tanzania, wilt is most likely caused by the fungal pathogen Macrophomina phaseolina, infecting roots. Infection of stems by Fusarium solani may also contribute to the wilt symptoms in Tanzania. Further artificial inoculation under controlled conditions confirmed that M. phaseolina can cause typical wilting symptoms on S. aethiopcium genotypes. The discovery of different putative causal agents of wilt in the crop demonstrates the need for site specific etiological analysis of wilt before developing and implementing effective control methods. Further research is needed to confirm the results and develop appropriate management measures against specific wilt pathogens.
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来源期刊
Frontiers in Agronomy
Frontiers in Agronomy Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
4.80
自引率
0.00%
发文量
123
审稿时长
13 weeks
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