Effectiveness Evaluation of Fenugreek Seeds Ethanol Extract (Trigonella foenum-graecum) Against Bacterial Growth Streptococcus sanguinis: In Vitro Study

Introduction: Streptococcus sanguinis is a pioneer bacteria in the formation of dental plaque that causes caries. Fenugreek (Trigonella-foenum-graecum L.) is a medicinal plant that has antibacterial properties because it contains alkaloids, tannins, flavonoids, glycosides, and terpenoids. The aim of this research was to determine the effectiveness of fenugreek seed extract with concentrations of 25%, 50%, 75%, and 100% on bacterial growth of S. sanguinis. Methods: This study is the post-test-only control group. The research sample is a pure culture of Streptococcus sanguinis bacteria ATCC 10556. Antibacterial testing uses the disc diffusion method by sight diameter bacterial inhibition zone.  Data was analyzed using the Kruskal-Wallis test. Results: The test results showed that the ethanol extract of fenugreek seeds with concentrations of 25%, 50%, 75%, and 100% had an inhibitory zone against the growth of Streptococcus sanguinis bacteria with the average diameter of the inhibitory zone for each concentration being 7.77 ± 6.13 mm. 9.90 ± 8.09, 12.22 ± 5.05, and 14.75 ± 4.83 mm, but the antibacterial effectiveness did not have a significant difference with a value of p = 0.12. Conclusion: The ethanol extract of fenugreek seeds has antibacterial activity against the Streptococcus sanguinis bacteria but is not significant.