单细胞RNA-Seq和大量RNA-Seq的综合分析揭示了急性髓性白血病肿瘤相关巨噬细胞的分子特征

IF 1.4 4区生物学 Q4 GENETICS & HEREDITY

Genetics research Pub Date : 2024-01-02 eCollection Date: 2024-01-01 DOI:10.1155/2024/5539065

Xin Gao

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引用次数: 0

摘要

背景：急性髓性白血病（AML）与巨噬细胞的关系仍有待深入探讨：急性髓性白血病（AML）与巨噬细胞之间的关系仍有待深入探讨：方法：从TCGA、GEO和TARGET数据库中收集急性髓性白血病患者的基因表达谱和临床变量特征。采用共识聚类法构建巨噬细胞相关聚类。利用 LASSO 和多变量 Cox 分析构建了巨噬细胞相关指数（MRI）。GSE71014 和 TARGET 数据集被用作外部验证集。采用急性髓细胞性白血病的单细胞测序数据（GSE116256）来分析细胞中的模型基因表达水平：结果：在急性髓细胞性白血病中构建了两个与巨噬细胞相关的集群，它们具有不同的预后和免疫浸润特征。结果：在急性髓细胞性白血病中构建了两个具有不同预后和免疫浸润特征的巨噬细胞相关群，群 B 的预后较差，具有更多的促癌通路富集和免疫抑制微环境。根据核磁共振成像，不同组别的患者表现出不同程度的免疫浸润、不同的突变和预后。LGALS1和BCL2A1可能在急性髓细胞白血病中起到促癌作用，而ELANE可能在抑癌方面有显著效果：结论：巨噬细胞相关基因（MRGs）对急性髓细胞白血病的发生和发展有重要影响。结论：巨噬细胞相关基因（MRGs）对急性髓细胞白血病的发生和发展有重要影响，磁共振成像可更好地评估急性髓细胞白血病患者的预后和免疫特征。

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Integrated Analysis of Single-Cell RNA-Seq and Bulk RNA-Seq Unravels the Molecular Feature of Tumor-Associated Macrophage of Acute Myeloid Leukemia.

Background: The association between acute myeloid leukemia (AML) and macrophage remains to be deeply explored.

Methods: Gene expression profiles and clinical variable characteristics of AML patients were collected from TCGA, GEO, and TARGET databases. Consensus clustering was employed to construct the macrophage-related clusters. The macrophage-related index (MRI) was constructed using the LASSO and multivariate Cox analysis. The GSE71014 and TARGET datasets were utilized as external validation sets. Single-cell sequencing data for AML (GSE116256) was adopted to analyze modeled gene expression levels in cells.

Results: Two macrophage-related clusters with different prognostic and immune infiltration characteristics were constructed in AML. Cluster B had a poorer prognosis, more cancer-promoting pathway enrichment, and an immunosuppressive microenvironment. Relied on the MRI, patients of different groups showed different levels of immune infiltration, different mutations, and prognoses. LGALS1 and BCL2A1 may play roles in promoting cancer in AML, while ELANE may have a significant effect on suppressing cancer.

Conclusion: Macrophage-related genes (MRGs) had significant impacts on the occurrence and progression of AML. MRI may better evaluate the prognosis and immune features of AML patients.

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来源期刊

Genetics research 生物-遗传学

自引率

6.70%

发文量

审稿时长

>12 weeks

期刊介绍： Genetics Research is a key forum for original research on all aspects of human and animal genetics, reporting key findings on genomes, genes, mutations and molecular interactions, extending out to developmental, evolutionary, and population genetics as well as ethical, legal and social aspects. Our aim is to lead to a better understanding of genetic processes in health and disease. The journal focuses on the use of new technologies, such as next generation sequencing together with bioinformatics analysis, to produce increasingly detailed views of how genes function in tissues and how these genes perform, individually or collectively, in normal development and disease aetiology. The journal publishes original work, review articles, short papers, computational studies, and novel methods and techniques in research covering humans and well-established genetic organisms. Key subject areas include medical genetics, genomics, human evolutionary and population genetics, bioinformatics, genetics of complex traits, molecular and developmental genetics, Evo-Devo, quantitative and statistical genetics, behavioural genetics and environmental genetics. The breadth and quality of research make the journal an invaluable resource for medical geneticists, molecular biologists, bioinformaticians and researchers involved in genetic basis of diseases, evolutionary and developmental studies.