鼠李糖乳杆菌 GG 通过肠道-血液轴改善高脂血症患者的牙周骨修复

Journal of dental research Pub Date : 2024-03-01 Epub Date: 2024-01-10 DOI:10.1177/00220345231217402
Y Huang, R Ge, J Qian, J Lu, D Qiao, R Chen, H Jiang, D Cui, T Zhang, N Wang, S He, M Wang, F Yan
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引用次数: 0

摘要

牙周骨再生仍是一项临床挑战,而高脂血症会加剧牙槽骨吸收。最近有报道称益生菌可改善骨量。我们旨在确定鼠李糖乳杆菌 GG(LGG)在牙周炎合并高脂血症的情况下对改善牙周骨再生的作用。构建了一个具有牙周炎、高脂血症和牙周瘘管缺损的 Sprague Dawley 大鼠模型(n = 36),给大鼠灌胃 LGG 6 周(随后大鼠被处死)。将供体大鼠灌胃 LGG 3 周后的粪便微生物群移植到受体大鼠体内,以评估 LGG 调节的肠道微生物群在牙周骨再生中的作用。使用微型计算机断层扫描和苏木精及伊红染色检测再生骨量。使用 16S 核糖体 RNA 测序分析肠道微生物群。采用液相色谱-质谱法检测血清代谢物(灌胃 LGG 6 周后)。筛选出的血清代谢物对骨髓间充质干细胞(BMMSCs)的促成骨作用在体外得到了验证。我们发现,灌胃 LGG 后,再生牙周骨的骨矿密度、骨量(BV)、骨小梁体积分数(BV/TV)和骨小梁厚度均有所增加(P < 0.05),但对口腔菌群的影响很小。灌胃 LGG 后,供体肠道中的葡萄球菌、棒状杆菌和柯林斯菌发生了显著变化,这些差异在受体中保持不变,受体再生牙周骨小梁厚度也有所增加(P < 0.05)。这些关键菌属与 BV/TV 和 BV 相关(P < 0.05)。此外,灌胃 LGG 能显著调节骨相关血液代谢物,其中硒蛋氨酸能促进 BMMSC 骨生成。值得注意的是,硒蛋氨酸与主要肠道菌属有关(P < 0.05)。总之,在牙周炎合并高脂血症的情况下,LGG通过调节肠道微生物群和增加血液中的促骨生成代谢物改善了牙周骨再生。这些结果揭示了使用益生菌通过肠道-血液-骨骼轴促进牙周骨骼再生的新见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Lacticaseibacillus rhamnosus GG Improves Periodontal Bone Repair via Gut-Blood Axis in Hyperlipidemia.

Periodontal bone regeneration remains a clinical challenge, and hyperlipidemia can aggravate alveolar bone resorption. Probiotics have recently been reported to improve bone mass. We aimed to determine the role of Lacticaseibacillus rhamnosus GG (LGG) in periodontal bone regeneration improvement within the context of periodontitis with hyperlipidemia. A Sprague Dawley rat model for periodontitis, hyperlipidemia, and periodontal fenestration defect was constructed (n = 36) and administered LGG gavage for 6 wk (the rats were subsequently sacrificed). Fecal microbiota from donor rats 3 wk after LGG gavage was transplanted into recipient rats to evaluate the role of LGG-modulated gut microbiota in periodontal bone regeneration. Regenerated bone mass was detected using micro-computerized tomography and hematoxylin and eosin stain. Gut microbiota was analyzed using 16S ribosomal RNA sequencing. Serum metabolites were detected by liquid chromatography-mass spectrometry (6 wk after LGG gavage). The pro-osteogenic effects of screened serum metabolite were verified in vitro on bone marrow mesenchymal stem cells (BMMSCs). We found that the bone mineral density, bone volume (BV), trabecular bone volume fraction (BV/TV), and trabecular thickness of the regenerated periodontal bone increased after LGG gavage (P < 0.05) but had little effect on oral flora. After LGG gavage, Staphylococcus, Corynebacterium, and Collinsella in the gut of donors were significantly changed, and these differences were maintained in recipients, who also showed increased trabecular thickness of the regenerated periodontal bone (P < 0.05). These key genera were correlated with BV/TV and BV (P < 0.05). In addition, LGG gavage significantly regulated bone-related blood metabolites, of which selenomethionine promoted BMMSC osteogenesis. Notably, selenomethionine was associated with key gut genera (P < 0.05). Collectively, LGG improved periodontal bone regeneration in the context of periodontitis with hyperlipidemia by modulating gut microbiota and increasing pro-osteogenic metabolites in the blood. These results reveal new insights into the use of probiotics to promote periodontal bone regeneration via the gut-blood-bone axis.

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