Investigation of Optimum Dose of Gamma Irradiation and Ems (Single and Combination Treatments) to Induce Mutation in Safflower (Carthamus tinctorius) Wild Cultivar

In induced mutation breeding program, it is necessary to find the optimum dose for inducing mutation. Effective dose is vary plant to plant depends on size of seeds. There is inverse relation size of seeds and concentration of mutagen. Parameter of consideration of optimum dose for mutagenesis is germination around 50 % of seeds after mutagenesis is indication of successive mutagenesis. In this mutagenesis investigation 50% seed germination is observed in treatment of gamma irradiation 300 Gy and EMS 0.3 %. Another great decrease in seed germination of 80 % is observed in combination treatments 300 Gy + 0.3 and 400 Gy + 0.3 %.