利用酶处理技术降解微污染物质阿莫西林并评估所产生的副产品

IF 1.1 Q4 CHEMISTRY, ANALYTICAL
G. Vettorello, Lucas Schmidt, Daniel Kuhn, Bruno da Silva, A. Weber, S. Cordeiro, Guilherme Henn, Bruna Costa, Jéssica dos Santos, Joana Willrich, Cristiano Pereira, Alexandre Rieger, C. Steffens, E. Ethur, E. D. de Freitas, L. Hoehne
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引用次数: 0

摘要

巴西现行的饮用水处理法规并不要求对抗生素阿莫西林等微污染物进行分析。然而,这些化合物在环境中的存在与细菌的抗药性有直接联系,因此有必要开发以去除这些化合物为重点的方法。已经提出了一些替代方法,如高级氧化工艺,最近的研究表明,某些酶,如过氧化物酶,有能力在过氧化氢(H2O2)的作用下降解微污染物。因此,本研究旨在利用过氧化物酶评估 25 mg L-1 阿莫西林的酶降解情况。具体方法是将阿莫西林溶液加入间歇式反应器,然后加入不同浓度的过氧化物酶和不同浓度的 H2O2(0.5、1.0 和 2.5 mmol L-1)。反应持续 9 小时。所有样品均采用液相色谱-质谱联用技术进行分析,并使用大型蚤对残留毒性进行评估。结果表明,药物降解率约为 50%,并发现了来自阿莫西林的副产品。毒理学测试表明,这些副产品对微型甲壳动物的毒性很小,这突出了评估拟议处理方法安全性的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Degradation of the Micropollutant Amoxicillin using Enzymatic Treatment and Evaluation of Resulting Byproducts
Current Brazilian legislation for the treatment of drinking water does not require analysis for micropollutants such as the antibiotic amoxicillin. However, the presence of these compounds in the environment is directly linked to bacterial resistance, and the development of methodologies focusing on their removal is necessary. A few alternatives, such as Advanced Oxidative Processes, have already been proposed and, more recently, studies have shown that certain enzymes, like peroxidases, have the ability to degrade micropollutants in the presence of hydrogen peroxide (H2O2). In this sense, the present study aims to evaluate the enzymatic degradation of 25 mg L-1 amoxicillin using peroxidase. For the specific method, amoxicillin solutions were fed to a batch reactor and different concentrations of peroxidase combined with varying H2O2 concentrations (0.5, 1.0, and 2.5 mmol L-1) were added. Reactions occurred for 9 hours. All samples were analyzed by liquid chromatography coupled with mass spectrometry, and the residual toxicity was assessed using Daphnia magna. The results showed around 50% degradation of the drug, and byproducts originating from amoxicillin were identified. Toxicological tests indicated that the byproducts were minimally toxic to the microcrustacean, highlighting the importance of evaluating the safety of proposed treatments.
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来源期刊
CiteScore
1.60
自引率
14.30%
发文量
46
期刊介绍: BrJAC is dedicated to the diffusion of significant and original knowledge in all branches of Analytical Chemistry, and is addressed to professionals involved in science, technology and innovation projects at universities, research centers and in industry.
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