精选精油对牛乳腺炎金黄色葡萄球菌的抗菌活性

Dairy Pub Date : 2024-01-05 DOI:10.3390/dairy5010005
Karen Vanessa Munive Nuñez, Anderson Clayton da Silva Abreu, Jaqueline Milagres de Almeida, J. L. Gonçalves, É. C. Bonsaglia, M. V. dos Santos, Nathália Cristina Cirone Silva
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引用次数: 0

摘要

金黄色葡萄球菌是奶牛亚临床乳腺炎的主要病因,它对抗生素产生的抗药性限制了治疗效果。精油(EO)是具有多种抗菌特性的天然产品,可用于治疗牛乳腺炎。本研究旨在调查从巴西圣保罗州亚临床牛乳腺炎病例中分离出的金黄色葡萄球菌对 EO 的抗菌活性。根据生物膜形成基因(icaA、icaD 和 bap)的存在情况,共筛选出 14 个金黄色葡萄球菌分离株,并将其培养至 103 CFU.mL-1 的最低抑菌浓度 (MIC) 和最低杀菌浓度 (MBC)。使用气相色谱-质量选择检测器(GC/MSD)对茴香油的化学成分进行了鉴定。罗勒和佛手柑环氧乙烷对金黄色葡萄球菌菌株的抗菌活性最高,平均 MIC/MBC 值分别为 1.561 ± 0.223/2.806 ± 0.255 mg.mL-1 和 2.782 ± 0.228/4.396 ± 0.198 mg.mL-1。罗勒环氧乙烷中的主要化合物是甲基辣椒素、芳樟醇和 α-胡麻烯,而佛手柑环氧乙烷则主要含有乙酸芳樟酯、柠檬烯和芳樟醇。这项研究强调了罗勒和佛手柑环氧乙烷作为天然抗菌剂治疗金黄色葡萄球菌引起的牛乳腺炎的潜力,为传统抗生素提供了潜在的替代品,有助于动物福利和公共卫生。此外,它还强调了进一步研究的必要性,以验证其长期效果、最佳剂量和应用方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antimicrobial Activity of Selected Essential Oils against Staphylococcus aureus from Bovine Mastitis
Staphylococcus aureus is a major cause of subclinical mastitis in dairy cows, and its development of antibiotic resistance has limited treatment efficacy. Essential oils (EOs) are natural products with a wide range of antimicrobial properties that could be used to treat bovine mastitis. This study aims to investigate the antimicrobial activity of EOs against S. aureus isolated from subclinical bovine mastitis cases in the State of São Paulo—Brazil. A total of 14 S. aureus isolates were selected, based on the presence of biofilm-forming genes (icaA, icaD, and bap), and were cultured to a final concentration of 103 CFU.mL−1 for the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) analysis of five EOs (Citrus aurantium bergamia—bergamot, Copaifera reticulata—copaiba, Foeniculum vulgare—fennel, Zingiber officinale—ginger, and Ocimum basilicum—basil). The chemical compositions of the EOs were characterized using gas chromatography coupled with a mass-selective detector (GC/MSD). Basil and bergamot EOs exhibited the highest antimicrobial activity against S. aureus strains, with mean MIC/MBC values of 1.561 ± 0.223/2.806 ± 0.255 mg.mL−1 and 2.782 ± 0.228/4.396 ± 0.198 mg.mL−1, respectively. The primary compounds in basil EO were methyl-chavicol, linalool, and α-humulene, while bergamot EO predominantly contained linalyl acetate, limonene, and linalool. This research highlights the potential of basil and bergamot EOs as natural antimicrobial agents for treating bovine mastitis caused by S. aureus, offering a potential alternative to traditional antibiotics and contributing to animal welfare and public health. In addition, it emphasizes the need for further studies to validate the long-term effects, optimal dosages, and application methods.
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