从草药中提取的天然异黄酮--福莫西汀对弓形虫有良好的抑制作用

Q3 Pharmacology, Toxicology and Pharmaceutics

Journal of HerbMed Pharmacology Pub Date : 2023-06-01 DOI:10.34172/jhp.2023.39

Hossein Mahmoudvand, Amal Khudair Khalaf, Nooshin Karbasian, Leila Masoori, Pouya Zareh Rajabi, M. Saki, Javad Ghasemian Yadegari

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引用次数: 0

摘要

简介甲萘素（FMN）是一种存在于许多植物中的天然异黄酮。这项研究考察了 FMN 对弓形虫的抗弓形虫作用和细胞毒性特性。研究方法通过细胞存活率检测法测定 FMN（2-64 µg/mL）对 48 小时内速虫形态的影响。还评估了不同浓度的 FMN 对巨噬细胞（J774-A1）的感染率、细胞内寄生虫和一氧化氮（NO）的影响。结果显示FMN 明显（P<0.001）降低了速生虫的存活率，IC50 值为 9.85 μg/mL。FMN 还降低了细胞内速虫的繁殖率，而 FMN 则增加了巨噬细胞中 FMN 的产生。结论本体外研究的结果表明，FMN 对弓形虫的速生体和胞内形式具有良好的抗弓形虫作用。虽然 FMN 的准确抗弓形虫机制尚不清楚，但我们的研究结果表明，触发 NO 的产生可能被认为是 FMN 控制和消灭弓形虫的主要机制之一。然而，在将 FMN 用于临床阶段之前，还必须进行进一步的调查，以评估 FMN 在动物模型中的效果，并评估其准确的作用机制。

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Promising effects of formononetin, a natural isoflavone derived from herbs, against Toxoplasma gondii

Introduction: Formononetin (FMN) is a natural isoflavone found in many plants. This work examined the anti-Toxoplasma effects and cytotoxicity properties of FMN on Toxoplasma gondii. Methods: Effects of FMN (2-64 µg/mL) on tachyzoites forms were measured by cell viability assay for 48 hours. The effects of different concentrations of FMN on infectivity rate, intracellular parasites, and nitric oxide (NO) in macrophage cells (J774-A1) were also evaluated. Results: FMN markedly (P<0.001) reduced the viability rate of tachyzoites forms with an IC50 value of 9.85 μg/mL. FMN also declined the rate of intracellular tachyzoites whereas, FMN increased the FMN production in macrophage cells. Conclusion: The results of the present in vitro study revealed the favorable anti-Toxoplasma effects of FMN against tachyzoites and intracellular forms of T. gondii. Although the accurate anti-Toxoplasma mechanisms of FMN are not clear, our results showed that triggering the NO production might be considered one of the main mechanism actions of FMN for controlling and eliminating T. gondii. However, further surveys are mandatory to assess the effects of FMN in animal models and to evaluate its accurate mechanism actions before its use in clinical phase.

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来源期刊

Journal of HerbMed Pharmacology Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

2.50

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Journal of Herbmed Pharmacology (J Herbmed Pharmacol) is the intersection between medicinal plants and pharmacology. This international journal publishes manuscripts in the fields of medicinal plants, pharmacology and therapeutic. This journal aims to reach all relevant national and international medical institutions and persons in electronic version free of charge. J Herbmed Pharmacol has pursued this aim through publishing editorials, original research articles, reviews, mini-reviews, commentaries, letters to the editor, hypothesis, case reports, epidemiology and prevention, news and views. In this journal, particular emphasis is given to research, both experimental and clinical, aimed at protection/prevention of diseases. A further aim of this journal is to emphasize and strengthen the link between herbalists and pharmacologists. In addition, J Herbmed Pharmacol welcomes basic biomedical as well as pharmaceutical scientific research applied to clinical pharmacology. Contributions in any of these formats are invited for editorial consideration following peer review by at least two experts in the field.