降低小直径血管移植物手术孔隙率的技术

E. A. Nemets, A. I. Khairullina, V. Y. Belov, V. A. Surguchenko, V. N. Vasilets, V. Sevastianov, E. Volkova, Y. Basok
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引用次数: 0

摘要

手术孔隙率(SP)过高是导致大量失血和血肿形成的原因之一。因此,降低小直径血管移植物(VGs)的孔隙率是一项至关重要的任务。这项工作的目的是开发一种技术,用于形成具有生物活性涂层的聚己内酯(PCL)基小直径血管移植物,并降低其孔隙率。在 NANON-01A 装置(日本 MECC 公司)上,通过电纺丝从 5%的 PCL 溶液中加入 5-30% 的明胶(PCL/G),制造出内径为 3 毫米的多孔 VG。在牛血清白蛋白、肝素和血小板裂解物溶液中依次培养 VG,然后在戊二醛溶液中固定,从而形成生物活性涂层。对样品的表面结构和机械性能进行了研究。根据生物活性 VG 与体外细胞培养物的相互作用,对其功能特性进行了评估。结果发现,在工作溶液中加入明胶可将 SP 从 30.4 ± 1.5 mL/(cm2 -min) 降低到 2.8 ± 0.5 ml/(cm2-min)。研究表明,当 PCL/明胶的比例为 9 : 1 时,生物活性 VG 样品的外侧和内侧具有表面均匀性(无缺陷)、接近相同直径血管的机械性能(杨氏模量 6.7 ± 2.1 兆帕、拉伸强度 26.7 ± 4.9 牛顿、断裂伸长率 423 ± 80%)以及支持人脐静脉内皮细胞系 EA.hy926 粘附和增殖的能力。在 PCL 溶液中加入 10%的明胶(按聚合物重量计)可降低小直径 VG 的 SP 值,使其内外表面均匀一致,改善其机械性能,但不会降低其支持血管内皮细胞粘附和增殖的能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Technique for reducing the surgical porosity of small-diameter vascular grafts
High surgical porosity (SP) is one of the causes of significant blood loss, as well as hematoma formation. So, reducing the SP of small-diameter vascular grafts (VGs) is a crucial task.The objective of this work was to develop a technology for the formation of polycaprolactone (PCL)-based small-diameter VGs with a bioactive coating with reduced SP.Materials and methods. Porous VGs with an inner diameter of 3 mm were fabricated by electrospinning from 5% PCL solution with addition of 5–30% gelatin (PCL/G) on an NANON-01A unit (MECC CO, Japan). Bioactive coating was applied by sequential incubation of VGs in solutions of bovine serum albumin, heparin and platelet lysate with fixation in a glutaric aldehyde solution. The surface structure and mechanical properties of the samples were investigated. Functional properties of the bioactive VGs were evaluated in relation to their interaction with cell cultures in vitro.Results. It was found that introduction of gelatin into the working solution reduces SP from 30.4 ± 1.5 mL/(cm2 ·min) to 2.8 ± 0.5 ml/(cm2 ·min). It was shown that at a PCL/gelatin ratio of 9 : 1, the outer and inner sides of the bioactive VGs samples are characterized by surface uniformity (no defects), mechanical properties close to blood vessels of the same diameter (Young’s modulus 6.7 ± 2.1 MPa, tensile strength 26.7 ± 4.9 N and elongation to break 423 ± 80%) and ability to support adhesion and proliferation of human umbilical vein endothelial cell line, EA.hy926.Conclusion. Introduction of 10% gelatin content (by the polymer weight) into PCL solution reduces the SP of small-diameter VGs, leads to uniformity in their inner and outer surface, improvement in their mechanical properties without reducing their ability to support adhesion and proliferation of vascular endothelial cells.
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