{"title":"检测 N-乙酰转移酶 2 (NAT2) 基因启动子区域单核苷酸多态性 (SNPs) 的 TaqMan 基因分型测定法","authors":"Kinasih Prayuni","doi":"10.33476/jky.v30i2.2121","DOIUrl":null,"url":null,"abstract":"The N-acetyltransferase 2 (NAT2) polymorphism in coding region has been studies intensively. However, there are limited studies for promoter region of NAT2 gene. Several reported study showed that the promoter region polymorphism of NAT2 gene is genotyped by PCR-sequencing approach. In this paper, we describe TaqMan based assays for the NAT2 polymorphism genotyping in promoter region with following SNP: rs4646243 [T>C], rs4646244 [T>A], rs4646267 [A>G], rs4345600 [A>G], and rs4646246 [A>G]. Our result showed a good separation cluster, trailing cluster and some mix cluster. TaqMan genotyping assay has shown a sensitivity and specificity to detect polymorphism in NAT2 promoter region.","PeriodicalId":347843,"journal":{"name":"Jurnal Kedokteran YARSI","volume":"72 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"TaqMan Genotyping Assay Method for Single Nucleotide Polymorphisms (SNPs) detection in promoter region of N-Acetyltransferase 2 (NAT2) gene\",\"authors\":\"Kinasih Prayuni\",\"doi\":\"10.33476/jky.v30i2.2121\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The N-acetyltransferase 2 (NAT2) polymorphism in coding region has been studies intensively. However, there are limited studies for promoter region of NAT2 gene. Several reported study showed that the promoter region polymorphism of NAT2 gene is genotyped by PCR-sequencing approach. In this paper, we describe TaqMan based assays for the NAT2 polymorphism genotyping in promoter region with following SNP: rs4646243 [T>C], rs4646244 [T>A], rs4646267 [A>G], rs4345600 [A>G], and rs4646246 [A>G]. Our result showed a good separation cluster, trailing cluster and some mix cluster. TaqMan genotyping assay has shown a sensitivity and specificity to detect polymorphism in NAT2 promoter region.\",\"PeriodicalId\":347843,\"journal\":{\"name\":\"Jurnal Kedokteran YARSI\",\"volume\":\"72 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-08-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Jurnal Kedokteran YARSI\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33476/jky.v30i2.2121\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jurnal Kedokteran YARSI","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33476/jky.v30i2.2121","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
TaqMan Genotyping Assay Method for Single Nucleotide Polymorphisms (SNPs) detection in promoter region of N-Acetyltransferase 2 (NAT2) gene
The N-acetyltransferase 2 (NAT2) polymorphism in coding region has been studies intensively. However, there are limited studies for promoter region of NAT2 gene. Several reported study showed that the promoter region polymorphism of NAT2 gene is genotyped by PCR-sequencing approach. In this paper, we describe TaqMan based assays for the NAT2 polymorphism genotyping in promoter region with following SNP: rs4646243 [T>C], rs4646244 [T>A], rs4646267 [A>G], rs4345600 [A>G], and rs4646246 [A>G]. Our result showed a good separation cluster, trailing cluster and some mix cluster. TaqMan genotyping assay has shown a sensitivity and specificity to detect polymorphism in NAT2 promoter region.
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