О. Prijma, N. Ohorodnyk, V. Krykunova, K. Suprunenko, L. Karysheva
{"title":"从家养山羊中分离出的食道线虫(线虫纲,强线虫目)的形态特征","authors":"О. Prijma, N. Ohorodnyk, V. Krykunova, K. Suprunenko, L. Karysheva","doi":"10.15421/10.15421/022360","DOIUrl":null,"url":null,"abstract":"The nematodes of the genus Oesophagostomum Molin, 1861 parasitize in the large intestine of ruminants and are widely represented in the terrestrial ecosystems of many countries of the world. One of the most common species of this genus in small ruminants is Oesophagostomum venulosum Rudolphi, 1809. The validity of this species was established using morphological and molecular genetic methods, the importance of which is still relevant. The aim of our research was to determine the morphological and metric characteristics of O. venulosum nematodes isolated from domestic goats in Ukraine. The differential parameters of mature males and females of nematodes of this species are described and illustrated, and the obtained data is analyzed in the context of the findings of other authors. The taxonomic morphological characters of O. venulosum include the presence of a pronounced cuticular vesicle at the head end, inner and external corona radiata, cervical papillae located behind the esophagus; in males, the presence of prebursal papillae in front of the tail bursa and features of its structure, thin and tubular spicules and features of the structure of their proximal and distal ends; in females, features of the structure of the tail end, vulva and its location. 35 morphometric indicators were determined and proposed to be used in the identification of O. venulosum in males, and 29 indicators in females. Of these, 20 parameters characterize the general body structure (the length and width of the nematode, the dimensions of the mouth capsule, cervical vesicle, esophagus, the length of the inner and outer radial crowns, the location of the nerve ring and cervical papillae, as well as their sizes). In male nematodes of this species, 15 indicators characterize the metric parameters of the reproductive system (the size of the spicules in their various parts, gubernaculum, genital cone, genital papillae and their location, the width of the copulatory bursa). In females, 7 indicators are described that characterize the length of the vagina, the ovipositor, the location of the vulva and anus, the width of the body in the area of the vulva and anus, as well as two metric parameters of eggs. The identified and described morphometric characters of O. venulosum males and females based on microscopic studies can be an economical and effective method for researchers to accurately identify nematodes of this species. The information obtained in this study can contribute to the timely planning of control and prevention strategies for the parasitism of these nematodes on goat farms.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":"62 1","pages":""},"PeriodicalIF":0.5000,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Morphometric characteristics of the nematode Oesophagostomum venulosum (Nematoda, Strongylida) isolated from the domestic goat\",\"authors\":\"О. Prijma, N. Ohorodnyk, V. Krykunova, K. Suprunenko, L. Karysheva\",\"doi\":\"10.15421/10.15421/022360\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The nematodes of the genus Oesophagostomum Molin, 1861 parasitize in the large intestine of ruminants and are widely represented in the terrestrial ecosystems of many countries of the world. One of the most common species of this genus in small ruminants is Oesophagostomum venulosum Rudolphi, 1809. The validity of this species was established using morphological and molecular genetic methods, the importance of which is still relevant. The aim of our research was to determine the morphological and metric characteristics of O. venulosum nematodes isolated from domestic goats in Ukraine. The differential parameters of mature males and females of nematodes of this species are described and illustrated, and the obtained data is analyzed in the context of the findings of other authors. The taxonomic morphological characters of O. venulosum include the presence of a pronounced cuticular vesicle at the head end, inner and external corona radiata, cervical papillae located behind the esophagus; in males, the presence of prebursal papillae in front of the tail bursa and features of its structure, thin and tubular spicules and features of the structure of their proximal and distal ends; in females, features of the structure of the tail end, vulva and its location. 35 morphometric indicators were determined and proposed to be used in the identification of O. venulosum in males, and 29 indicators in females. Of these, 20 parameters characterize the general body structure (the length and width of the nematode, the dimensions of the mouth capsule, cervical vesicle, esophagus, the length of the inner and outer radial crowns, the location of the nerve ring and cervical papillae, as well as their sizes). In male nematodes of this species, 15 indicators characterize the metric parameters of the reproductive system (the size of the spicules in their various parts, gubernaculum, genital cone, genital papillae and their location, the width of the copulatory bursa). In females, 7 indicators are described that characterize the length of the vagina, the ovipositor, the location of the vulva and anus, the width of the body in the area of the vulva and anus, as well as two metric parameters of eggs. The identified and described morphometric characters of O. venulosum males and females based on microscopic studies can be an economical and effective method for researchers to accurately identify nematodes of this species. 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引用次数: 0
摘要
Oesophagostomum Molin, 1861 属的线虫寄生在反刍动物的大肠中,广泛分布于世界许多国家的陆地生态系统中。Oesophagostomum venulosum Rudolphi, 1809 是该属在小反刍动物中最常见的物种之一。该物种的有效性是通过形态学和分子遗传学方法确定的,其重要性依然存在。我们的研究目的是确定从乌克兰家养山羊中分离出的 O. venulosum 线虫的形态和计量特征。我们描述并说明了该物种成熟雄性和雌性线虫的不同参数,并结合其他作者的研究结果对获得的数据进行了分析。O.venulosum的分类形态特征包括:头端有明显的角质囊、内部和外部放射状副晕、位于食道后方的颈乳头;雄性尾囊前方有前囊乳头及其结构特征、细管状棘突及其近端和远端的结构特征;雌性尾端、外阴及其位置的结构特征。已确定并建议用于识别雄性 O. venulosum 的 35 个形态指标和雌性 O. venulosum 的 29 个指标。其中 20 个参数描述了一般身体结构(线虫的长度和宽度,口囊、颈囊、食道的尺寸,内外径冠的长度,神经环和颈乳头的位置及其大小)。在该物种的雄性线虫中,有 15 个指标描述了生殖系统的度量参数(各部分棘突的大小、生殖器锥体、生殖器乳头及其位置、交配囊的宽度)。在雌性方面,描述了 7 项指标,包括阴道长度、产卵器、外阴和肛门的位置、外阴和肛门区域的身体宽度,以及卵的两个度量参数。基于显微镜研究对 O. venulosum 雌雄形态特征的鉴定和描述,是研究人员准确鉴定该物种线虫的一种经济而有效的方法。本研究获得的信息有助于及时规划山羊养殖场对这些线虫寄生的控制和预防策略。
Morphometric characteristics of the nematode Oesophagostomum venulosum (Nematoda, Strongylida) isolated from the domestic goat
The nematodes of the genus Oesophagostomum Molin, 1861 parasitize in the large intestine of ruminants and are widely represented in the terrestrial ecosystems of many countries of the world. One of the most common species of this genus in small ruminants is Oesophagostomum venulosum Rudolphi, 1809. The validity of this species was established using morphological and molecular genetic methods, the importance of which is still relevant. The aim of our research was to determine the morphological and metric characteristics of O. venulosum nematodes isolated from domestic goats in Ukraine. The differential parameters of mature males and females of nematodes of this species are described and illustrated, and the obtained data is analyzed in the context of the findings of other authors. The taxonomic morphological characters of O. venulosum include the presence of a pronounced cuticular vesicle at the head end, inner and external corona radiata, cervical papillae located behind the esophagus; in males, the presence of prebursal papillae in front of the tail bursa and features of its structure, thin and tubular spicules and features of the structure of their proximal and distal ends; in females, features of the structure of the tail end, vulva and its location. 35 morphometric indicators were determined and proposed to be used in the identification of O. venulosum in males, and 29 indicators in females. Of these, 20 parameters characterize the general body structure (the length and width of the nematode, the dimensions of the mouth capsule, cervical vesicle, esophagus, the length of the inner and outer radial crowns, the location of the nerve ring and cervical papillae, as well as their sizes). In male nematodes of this species, 15 indicators characterize the metric parameters of the reproductive system (the size of the spicules in their various parts, gubernaculum, genital cone, genital papillae and their location, the width of the copulatory bursa). In females, 7 indicators are described that characterize the length of the vagina, the ovipositor, the location of the vulva and anus, the width of the body in the area of the vulva and anus, as well as two metric parameters of eggs. The identified and described morphometric characters of O. venulosum males and females based on microscopic studies can be an economical and effective method for researchers to accurately identify nematodes of this species. The information obtained in this study can contribute to the timely planning of control and prevention strategies for the parasitism of these nematodes on goat farms.