小麦(Triticum aestivum L.)产量及其贡献性状转基因分离株的鉴定和遗传评估

Parul Gupta, Ravindra Prasad, Mohit Sharma
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摘要

2018-19年和2019-20年Rabi期间,在BHU农业科学研究所农业研究农场研究了两个杂交种,即K 1006 x LOK 1和PBW 343 x HUW 234,这两个杂交种来自四个不同的亲本,目的是在分离F2和F3群体中分别鉴定和评估产量/株及其贡献性状的强健转基因分离株(TS)。研究结果表明,在谷物产量/株方面,两个杂交群体中的转基因个体都超过了亲本(9% 到 9.52%)。在 PBW 343 x HUW 234 杂交中,AL 的 TS 频率最高(74.29%),在 K 1006 x LOK 1 杂交中,1000 粒重的 TS 频率较高(72%)。在 PBW 343 x HUW 234(89.5%)的 F2 中,谷粒产量/株的同期 TS 数量最多,其次是 K 1006 x LOK 1(79.0%)。在 K 1006 x LOK 1 杂交品种中,谷粒产量与 SLPS、GPS 和 1000 GW 同时发生转基因的频率很高,在 PBW 343 x HUW 234 杂交品种中,谷粒产量与 NET 和 AL 同时发生转基因的频率也很高。因此,可以推测谷物产量依赖于这些性状,或者这些性状的基因之间可能存在连锁阻力,从而使责任基因一起遗传。F2 中最有希望出现 TS 标记的是 K 1006 x LOK 1 中的 36 号植株和另一个杂交品种中的 30 号、68 号和 100 号植株。基于高频率的 TS,可以推断转基因育种是通过恢复转基因分离株来提高作物产量和其他理想性状的绝佳工具。
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Identification and genetic assessment of transgressive segregants for yield and its contributing traits in wheat (Triticum aestivum L.)
Two crosses viz., K 1006 x LOK 1 and PBW 343 x HUW 234 derived from four diverse parents were examined during Rabi 2018-19 and 2019-20 at Agricultural Research Farm, Institute of Agricultural Sciences, BHU in order to identify and assess the robust transgressive segregants (TS) in the segregating F2 and F3 population for yield/plant and its contributing traits respectively. Findings reveal that individuals transgressed beyond the parents in both the crosses for grain yield/plant (9 to 9.52 %). The maximum frequency of TS are found for AL (74.29%) in the PBW 343 x HUW 234 and it was higher for 1000 grain weight (72%) in the K 1006 x LOK 1 cross. The highest number of simultaneous TS for grain yield/plant was found in the F2 for the PBW 343 x HUW 234 (89.5%) followed by K 1006 x LOK 1 (79.0%). The frequency of simultaneous transgression for grain yield coupled with SLPS, GPS, 1000 GW in K 1006 x LOK 1 cross along with NET and AL in PBW 343 x HUW 234 cross was found very frequently. Hence, it is presumed that either grain yield is dependent on these traits or there may be linkage drag among the genes for such traits so that responsible gene(s) could be inherited together. The most promising TS tagged in F2’s were plant No. 36 in the K 1006 x LOK 1 and plant No. 30, 68 and 100 in the other cross. Based on high frequency of TS, it is inferred that transgressive breeding could be used as an excellent tool to improve the crop yield and other desirable traits by recovering the transgressive segregants.
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