筛选从杜仲提取物中提取的植物纳米硒复合材料对降低男性不育症的可能影响

IF 0.5 Q4 BIOLOGY
Q. S. Basman, I. A. Huda, J. A. Shayma’a
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引用次数: 0

摘要

通过同时或连续使用纳米硒抗氧化植物提取物,可以避免或减轻酮康唑的抗生育作用。使用硒纳米颗粒(SeNPs)是增强其治疗效果和降低毒性的重要方法之一。本研究旨在分析服用抗氧化植物纳米硒以减轻酮康唑带来的氧化损伤后,雄激素(如睾酮和促性腺激素激素:促黄体生成素和促卵泡生成素)参数以及精子指数的变化。简而言之,在磁力搅拌器上(50 °C,PH 9),将每体积重量 1%的提取物按不同比例加入 10 mM 亚硒酸钠溶液中,在黑暗中放置 12 小时,再放置 48 小时,然后用紫外-可见光谱(UV-vis spectroscopy,UV-vis spectroscopy)、傅立叶变换红外光谱(Fourier transform infrared spectroscopy,FTIR)和动态光散射(Dynamic light scattering,DLS)进行表征。选定含有最佳硒纳米粒子的溶液后,按以下方法给七组白化大鼠中的三组(每组八只)注射硒纳米粒子:组BC、BD、BE1、BE2 和 BE3;每组口服酮康唑 50 毫克/千克,连续 14 天,然后再口服 200 毫克/千克白花蛇舌草:200 毫克/千克白花蛇舌草(Gr. BC)、0.5 毫克/千克口服亚硒酸钠(Gr. BD)、0.5 毫克/千克/平方厘米皮肤面积局部纳米硒(Gr. BE1)、0.25 毫克/千克口服纳米硒(Gr. BE2)和 0.5 毫克/千克口服纳米硒(Gr. BE3),分别持续 28 天。实验结束后,对动物进行麻醉,使用Elisa试剂盒对血浆中的睾酮、促黄体生成素和促卵泡生成素进行评估;然后对动物实施安乐死,仔细取出右侧睾丸的附睾,对精子指数(数量、存活率、畸形率和活力)进行评估。在加入酸性亚硒酸钠溶液并反应 12 小时后,硒离子从深棕色逐渐变为浅黄色,然后变为橘红色,这证实了白花蛇舌草提取物以(1:2)(Na2SeO3:白花蛇舌草)溶液诱导硒离子还原成 PF-SeNPs。在紫外-可见光谱中,在 268-964 纳米之间出现了一个强烈的吸收峰,最大值在 268 纳米,证实了纳米硒的形成。经 DLS 验证,植物制备的最佳纳米硒粒子呈球形,稳定性高,尺寸最小(直径 39.4 nm),聚分散指数为 0.242,zeta 电位值为 -56.57 mV。在目前的研究中,酮康唑的剂量为 50 毫克/千克/天,口服 14 天,睾丸受到损伤。这种睾丸损伤导致睾酮水平显著降低,促甲状腺激素(LH)和促甲状腺激素(FSH)水平升高,精子数量、活力和存活率显著下降,进而影响精子发生。与此同时,在服用酮康唑后服用欧车前提取物、亚硒酸钠和纳米硒溶液(不同剂量和途径)可显著改善生化指标。这些改善包括睾酮水平的提高,对 LH 和 FSH 水平几乎没有影响,以及精子指数的改善。此外,0.25 毫克/千克和 0.50 毫克/千克的纳米硒口服组效果最好,两者之间只有微小差异。总之,以纳米硒为基础的植物提取物能显著改善睾丸组织的抗氧化效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Screening the possible effect of a phytofabricated nanoselenium-composite from Eruca sativa extract in reducing infertility in males
The antifertility effects of ketoconazole can be avoided or diminished by administering nano-selenium-based-antioxidant plant extract simultaneously or sequentially. Using selenium as nanoparticles (SeNPs) is one of the essential methods for enhancing its therapeutic effects and lowering toxicities. This study aimed to analyze the changes made to the parameters of androgens, such as testosterone and gonadotropin hormones: luteinizing hormone and follicle stimulating hormones together with sperm indexes after administration of antioxidant phytofabricated nanoselenium to mitigate oxidative damage brought on by ketoconazole. In brief, 1% weight-per-volume of the extract was loaded into a solution of 10 mM sodium selenite in various ratios on a magnetic stirrer (50 °C, PH 9) in the dark for 12 hours, left for 48 hours and then sent for characterization, which was performed using ultraviolet-visible spectroscopy (UV-vis spectra), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS). Then after the selection of the solution containing the optimal fabricated selenium nanoparticles it was administered to three groups out of seven groups of albino rats with eight animals in each one as follows: Gr. A negative control (no treatment), Gr. B oral ketoconazole 50 mg/kg for fourteen days, Gr. BC, BD, BE1, BE2 and BE3; each one received oral ketoconazole 50 mg/kg for fourteen days followed by: 200 mg/kg Eruca sativa (Gr. BC), 0.5 mg/kg oral sodium selenite (Gr. BD), 0.5 mg/kg/cm2 skin area local nanoselenium (Gr. BE1), 0.25 mg/kg oral nanoselenium (Gr. BE2) and 0.5 mg/kg oral nano selenium (Gr. BE3) respectively for 28 days. After this period, the animals were anesthetized, and plasma testosterone, luteinizing, and follicle stimulating hormones were assessed using Elisa Kit; after that, they were euthanized, and the epididymis of the right testis was carefully removed for evaluation of sperm indices (count, viability, abnormality, and motility). The reduction of selenium ions into PF-SeNPs induced by Eruca sativa extracts at a ratio of (1:2) (Na2SeO3: Eruca sativa) solution was confirmed by the gradual conversion of colour from dark brown to light yellow and then to reddish-orange after the addition of acidic sodium selenite solution and reacting for 12 h. The final reddish-orange colour is the most significant property of nanoparticles. In UV-vis spectroscopy, a strong absorption peak appeared between 268–964 nm with maxima at 268 nm, confirming the formation of nanoselenium. The optimal phytofabricated nanoselenium particles were obtained with a spherical shape, highly stable, and the smallest in size (39.4 nm in diameter) as proved by DLS with Poly Dispersity Index of 0.242 and zeta potential value of –56.57 mV. In the current study, testicles were damaged by administering ketoconazole at a dose of 50 mg/kg/day orally for 14 days. This testicular damage was linked to significant reductions in testosterone levels, elevated levels of LH and FSH, and significant decreases in sperm count, motility, and viability, which in turn affected spermatogenesis. Concurrently, the administration of Eruca sativa extract, sodium selenite and nanoselenium solution (in different doses and routes) following ketoconazole was shown to significantly improve biochemical parameters. These improvements included an increase in testosterone levels with little to no impact on LH and FSH levels as well as improved sperm indices. Additionally, the oral nanoselenium groups in 0.25 and 0.50 mg/kg produced the best outcomes with only minor differences between them. In conclusion, the antioxidant effects of the phytofabricated nanoselenium-based Eruca sativa leaf extract considerably improved testicular tissues.
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