不同基因型的儿童和青少年血液中脂肪因子和肌肉因子的含量与副氧合酶-1基因的多态性rs662有关

Q3 Medicine

Obesity and Metabolism Pub Date : 2023-08-25 DOI:10.14341/omet13006

A. Shestopalov, V. Davydov, G. T. Tumanyan, E. Teplyakova, T. Shkurat, E. V. Mashkina, M. Shkurat, A. M. Gaponov, O. V. Borisenko, S. Roumiantsev

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引用次数: 0

摘要

背景。在导致肥胖的众多原因中，遗传因素占有特殊的地位。其中，脂质代谢酶（包括副氧合酶-1 (PON-1)）的遗传多态性具有明显的作用。迄今为止，PON-1 多态性与间质组织内分泌功能状态之间的关系特征仍不清楚。对它的研究将阐明儿童和青少年肥胖症发生的微妙机制。该研究旨在探讨 PON-1 多态性（rs662）与不同性别肥胖儿童和青少年体内脂肪因子、肌动蛋白含量及血脂代谢变化之间的关系。材料与方法：在 100 名不同性别的健康儿童和青少年及其 89 名肥胖同龄人中开展了一项遗传学研究，以评估 PAO-1 （rs662）基因的单核苷酸多态性。用光度法测定了血清中的总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、超低密度脂蛋白胆固醇、三酰甘油、葡萄糖和转氨酶活性（丙氨酸转氨酶和天门冬氨酸转氨酶）、此外，还采用多重酶联免疫吸附法测定瘦素、脂肪连素、抵抗素、凋亡素、鸢尾素、肾上腺素、肌促生长素、FGF21、骨促生长素、胰高血糖素和胰岛素，采用酶联免疫吸附法测定阿司匹林。结果等位基因为 Arg192/Arg 的同卵双生患者中，男孩肥胖并发症的发生率较低，女孩肥胖并发症的发生率较高。在 PON-1 多态性的其他变体（Gln192/Gln 和 Gln192/Arg基因型）中，女孩体内形成了旨在预防肥胖并发症的保护机制。在具有 Gln192/Gln 基因型的男孩中，肥胖会导致脂质代谢发生更明显的变化，表现为脂肪组织质量的改变和增加，而在具有杂合 Gln192/Arg 等位基因的男孩携带者中，动脉粥样硬化过程会增加。副氧合酶-1基因（rs662）的多态性导致了儿童和青少年肥胖期间血液中脂肪因子和肌肉因子含量变化的性别差异。

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The content of adipokines and myokines in the blood of children and adolescents with different genotypes according to the polymorphism rs662 of the paraoxonase-1 gene

BACKGROUND. Among the many causes of obesity, genetic factors occupy a special place. An obvious role among them belongs to the genetic polymorphism of lipid metabolism enzymes, including paraoxonase-1 (PON-1). Until now, the character of the relationship between PON-1 polymorphism and the state of the endocrine function of mesenchymal tissues remains unclear. Its study will clarify the subtle mechanisms of the development of obesity in childhood and adolescence.AIM. The aim of the study was to investigate the relationship between PON-1 polymorphism (rs662) and changes in the content of adipokines, myokines, and blood lipid metabolism in children and adolescents of different sexes with obesity.MATERIALS AND METHODS. In 100 healthy children and adolescents of different sexes and 89 of their peers with obesity, a genetic study was conducted to assess the single nucleotide polymorphism of the PAO-1 (rs662) genes. In blood serum, total cholesterol, HDL cholesterol, LDL cholesterol, VLDL cholesterol, triacylglycerols, glucose and aminotransferase activity (alanine aminotransferase and aspartate aminotransferase) were determined by photometric methods, as well as leptin, adiponectin, resistin, apelin, irisin, adipsin, myostatin, FGF21, osteocrine, oncostatin and insulin — by multiplex ELISA, and asprosin — by ELISA ones.RESULTS. The patients with the homozygous Arg192/Arg allele, the development of complications of obesity in boys is limited and their occurrence in girls is prevented. In other variants of PON-1 polymorphism (Gln192/Gln and Gln192/Arg genotypes), protective mechanisms are formed in the body of girls aimed at preventing complications in obesity. In boys with the Gln192/Gln genotype, obesity reveals more pronounced shifts in lipid metabolism, manifestations of alteration and an increase in the mass of adipose tissue, and in boys-carriers of the heterozygous Gln192/Arg allele, atherogenesis processes increase.CONCLUSION. Polymorphism of the paraoxonase-1 gene (rs662) contributes to the appearance of gender differences in changes in the content of adipokines and myokines in the blood during obesity in childhood and adolescence.

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来源期刊

Obesity and Metabolism Medicine-Internal Medicine

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： Journal "Obesity and Metabolism" is a multidisciplinary forum for clinical and applied research in the field of biochemistry, physiology, pathophysiology, genetics, nutrition, as well as molecular, metabolic, psychological and epidemiological aspects of obesity and metabolism. The main subject "Metabolism" reviewed in the journal, includes fat, carbohydrate, protein, bone, fluid and electrolyte and other types of metabolism in the spectrum of pathology of the endocrine system. The priority direction of Journal "Obesity and Metabolism" is publishing modern high-quality original research on the effectiveness of new and existing treatments in any aspect of metabolic and endocrine diseases. Pre-clinical pharmacology, pharmacokinetics studies, meta-analyzes, addressed to drug safety and tolerance are also welcome for publication in the journal "Obesity and metabolism." Journal "Obesity and Metabolism" announces review articles that are balanced, clear and offer the reader a modern and critical analysis of the literature on the subject of the magazine. Case reports, and lecture materials are also published for highlighting for practitioners new approaches to diagnosis and treatment of patients with metabolic disorders and obesity.