异体输注红细胞后受体兔体液免疫和细胞免疫的状况

IF 0.5 Q4 BIOLOGY
M. Malyuk, O. Yehorov, I. O. Kharkevych, T. L. Savchuk, V. V. Klymchuk
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引用次数: 0

摘要

研究异体输注红细胞后受体兔体液免疫环节的激活情况对于从根本上了解模式动物免疫的形成非常重要。我们测定了受体兔血液中 B 淋巴细胞、M、G、A 三类免疫球蛋白、循环免疫复合物的含量以及淋巴细胞的抗体依赖性细胞毒性活性。兔子血液中 B 淋巴细胞的数量是根据 Bianco 的方法测定的。兔子血清中的免疫球蛋白含量是根据曼西尼法测定的。分光光度法用于测量循环免疫复合物。用比色法确定了淋巴细胞的抗体依赖性细胞毒性活性。对 5 只临床健康家兔进行了红细胞质量输注模型试验,方法是静脉注射同种异体红细胞质量,剂量为 5.5 mL/kg(体重)。研究材料为输血后第 3 天、第 7 天和第 23 天采集的血液样本。我们发现,在整个研究过程中,受试兔子血液中的 B 淋巴细胞含量都在增加:第 3 天,B 淋巴细胞含量比初始状态(11.4%)增加了 1.94 倍,为 22.2%;第 7 天,B 淋巴细胞含量增加了 2.22 倍,为 25.4%;实验第 23 天,淋巴细胞含量比初始状态增加了 2.46 倍,相当于 28.6%。输注红细胞后第 3 天,IgM 含量增加了 54.9%,为 2.20 ± 0.70 克/升。第 7 天,免疫球蛋白含量增加了 19.0%,为 1.69 ± 0.44 克/升,第 23 天,免疫球蛋白含量比初始状态(1.42 ± 0.18 克/升,完整兔子)减少了 54.2%,为 0.77 ± 0.25 克/升。在实验的第 3 天、第 7 天和第 23 天,G 类免疫球蛋白的含量几乎与初始状态相同。A类免疫球蛋白的含量在实验第3天下降了4.16倍,之后接近对照组的数值。给受体兔输注红细胞后,在整个研究过程中,循环免疫复合物的含量都比初始状态高:第 3 天,CICs 含量增加了 2.13 倍,达到 6.40 个常规单位,第 7 天和第 23 天,CICs 含量接近对照组的初始值。输血后第 3 天,兔子红细胞质量的血液淋巴细胞的抗体依赖性细胞毒性活性比初始状态(15.9%)增加,达到 17.3%;第 7 天为 19.4%;第 23 天增加到 27.9%。结果表明,受体兔在接受异体输注红细胞后出现了特征性免疫变化,这是体液免疫环节活性逐渐增强的结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The state of the humoral and cellular links of immunity of recipient rabbits following allogeneic transfusion of erythrocyte mass
The study of activation of the humoral link of immunity of recipient rabbits following allogeneic transfusion of erythrocyte mass is important for a fundamental understanding of the formation of immunity in model species of animals. We measured the contents of B-lymphocytes, immunoglobulins of M, G, A classes, circulating immune complexes in blood of the recipient rabbits and antibody-dependent cytotoxic activity of lymphocytes. Number of B-lymphocytes in the blood of the rabbits was determined according to Bianco. Immunoglobulin content in blood serum of the rabbits was measured based on the Mancini method. The spectrophotometry method was used to measure circulating immune complexes. The antibody-dependent cytotoxic activity of lymphocytes was identified by the colorimetric method. Modeling of erythrocyte mass transfusion was performed on five clinically healthy rabbits by intravenous injection of allogeneic erythrocyte mass in the dose of 5.5 mL/kg of body weight. The materials for the study were the blood samples, gathered on the 3rd, 7th, and 23rd days after transfusion. We found that the content of B-lymphocytes in blood of the recipient rabbits increased throughout the research: on the 3rd day the content of B lymphocytes, compared with the initial state (11.4%), increased by 1.94 times and was 22.2%; on the 7th day their content increased by 2.22 times and was 25.4%; and on the 23rd day of the experiment the content of lymphocytes increased by 2.46 times, compared with the initial state, equaling 28.6%. The content of IgM on the 3rd day after transfusion of erythrocyte mass increased by 54.9% and was 2.20 ± 0.70 g/L. On the 7th day it increased by 19.0%, equaling 1.69 ± 0.44 g/L, and on the 23rd day the content of immunoglobulins decreased by 54.2% compared with the initial state (1.42 ± 0.18 g/L, intact rabbits), accounting for 0.77 ± 0.25 g/L. The content of immunoglobulins of class G on the 3rd, 7th, and 23rd days of the experiment was nearly the same as in the initial state. The content of class A immunoglobulins decreased 4.16-fold on the 3rd day of the experiment, approaching the control values afterwards. After the transfusion of erythrocyte mass to the recipient rabbits, the content of circulating immune complexes was higher compared with the initial state throughout the research: on the 3rd day the CICs content increased by 2.13 times, measuring 6.40 conventional units, and on the 7th and 23rd days it approached the initial values of the control group. On the 3rd day after transfusion, the antibody-dependent cytotoxic activity of blood lymphocytes of erythrocyte mass of the rabbits increased compared with the initial state (15.9%), measuring 17.3%; on the 7th day it was 19.4%; and on the 23rd day it increased to 27.9%. The results revealed presence of characteristic immunological changes after allogeneic transfusion of erythrocyte mass to the recipient rabbits, which were a consequence of progressive increase in the activity of the humoral link of immunity.
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来源期刊
CiteScore
0.90
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