学生竞赛(知识生成) ID 1987946

IF 2.4 Q1 REHABILITATION
N. Punjani, Sighild Lemarchant, Svetlana Altamentova, J. Chio, Jian Wang, Yann Godfrin, M. G. Fehlings
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引用次数: 0

摘要

NX210c 是一种 12 氨基酸肽,来源于脊髓膜下器官-spondin 中保守的 thrombospondin 1 型重复序列,具有独特的多功能作用机制,可改善神经损伤后的预后。本研究旨在评估 NX210c 在颈椎创伤性脊髓损伤(SCI)模型中促进功能恢复和组织修复的功效。 成年雌性Wistar大鼠受到C6/C7夹片压迫-灌注损伤,从损伤后4小时或8小时开始,每天腹腔注射一次NX210c(8毫克/千克)或其载体,连续治疗8周(16-17只/组),同时进行神经行为测试。 较早在4小时给药NX210c可增加前肢握力(p<0.05),并改善运动的多个静态和动态方面,包括规律性指数和前肢支撑基础(CatWalk)(p<0.05)。将 NX210c 的首次给药时间延迟至 8 小时可促进体重增加,加快膀胱控制能力在伤后 14 到 9 天的恢复,并在伤后一周内改善躯干平衡(倾斜面)(p<0.05)。与 75% 的药物对照组相比,94% 的 NX210c 治疗大鼠在初始注射延迟时间点观察到体重支持。组织学研究(n=6/组)显示,与对照组相比,NX210c 在损伤后 8 小时开始治疗时,损伤中心的白质保存更完好,空腔缩小,尾部神经元体细胞数增加(p<0.05)。 NX210c针对SCI的各个方面,可改善运动功能、膀胱控制、白质保存和神经元数量,在较晚的初始注射时间点可观察到更多益处。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Student Competition (Knowledge Generation) ID 1987946
NX210c is a 12-amino acid peptide derived from conserved thrombospondin type 1 repeat sequences in the subcommissural organ-spondin, which has a unique multifunctional mechanism of action to ameliorate outcomes following neurological injuries. The aim of this study was to evaluate the efficacy of NX210c to promote functional recovery and tissue repair in a cervical traumatic spinal cord injury (SCI) model. Adult female Wistar rats were subjected to a C6/C7 clip compression-contusion injury and treated once daily with intraperitoneal injections of NX210c (8 mg/kg) or its vehicle for 8 weeks, beginning 4 hours (h) or 8h post-injury (n=16-17/group), with concurrent neurobehavioural tests. Earlier NX210c administration at 4h increased forelimb grip strength (p<0.05) and improved several static and dynamic aspects of locomotion including regularity index and base of support of the forelimbs (CatWalk) (p<0.05). Delaying initial administration of NX210c to 8h, promoted weight gain, accelerated bladder control recovery from 14 to 9 days post-injury, and improved trunk balance (inclined plane) as early as one-week post-injury (p<0.05). 94% of NX210c-treated rats compared to 75% of vehicle controls observed weight support at the delayed initial injection timepoint. Histology (n=6/group) demonstrated greater white matter preservation and reduced cavity size at the injury epicenter, and higher neuronal soma counts caudally, with NX210c starting 8h post-injury compared to the vehicle (p<0.05). NX210c targets various aspects of SCI, improving motor function, bladder control, white matter preservation, and neuronal counts, with more benefits observed at the later initial injection timepoint.
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来源期刊
CiteScore
3.20
自引率
3.40%
发文量
33
期刊介绍: Now in our 22nd year as the leading interdisciplinary journal of SCI rehabilitation techniques and care. TSCIR is peer-reviewed, practical, and features one key topic per issue. Published topics include: mobility, sexuality, genitourinary, functional assessment, skin care, psychosocial, high tetraplegia, physical activity, pediatric, FES, sci/tbi, electronic medicine, orthotics, secondary conditions, research, aging, legal issues, women & sci, pain, environmental effects, life care planning
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