学生竞赛(知识生成) ID 1985158

IF 2.4 Q1 REHABILITATION
Carly L O’Sullivan, Neil Tyreman, Rofyontsa F. Shanti, Wilbur A. O’Steen, Soroush Mirkiani, Maxwell Boakye, Dena R. Howland, Vivian K. Mushahwar
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引用次数: 0

摘要

脊髓损伤(SCI)会影响运动能力和生活质量。目前正在研究两种脊髓刺激方法,用于恢复脊髓损伤后的站立和行走:硬膜外脊髓刺激(ESCS)和椎管内微刺激(ISMS)。在 ESCS 中,电极被放置在硬脊膜上,而在 ISMS 中,超细线被插入脊髓内。这些方式可能通过不同的途径激活腹侧角的运动区。 本研究的目的是研究ESCS和ISMS激活的神经元分布和神经元类型的差异。 第一步是建立所需的免疫组化(IHC)染色方案。家猪被分为幼稚组(n=2)和阳性对照组(n=1)。天真对照组动物被麻醉 5 小时。阳性对照组动物麻醉 2 小时并在后肢肌肉注射高渗盐水。然后将动物安乐死,取出脊髓进行 IHC 分析。分析中使用了针对神经元活化标志物 cFos 和神经元标记物 NeuN 的抗体。 初步结果表明,ESCS 可激活背角的神经元,并在中间和腹侧区域激活零星的神经元。ISMS 主要激活运动相关网络所在的中间和腹侧区域的神经元。 据我们所知,这是首次研究 ESCS 和 ISMS 的激活类型和部位。这将为我们了解这些刺激模式的作用机制提供基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Student Competition (Knowledge Generation) ID 1985158
Spinal cord injury (SCI) affects locomotion and quality of life. Two spinal cord stimulation approaches are currently under investigation for restoring standing and walking following SCI: epidural spinal cord stimulation (ESCS) and intraspinal microstimulation (ISMS). In ESCS, electrodes are placed on the dura mater and in ISMS, ultrafine wires are inserted into the cord. These modalities likely activate the locomotor regions in the ventral horn through different pathways. The goal of this study is to examine the difference in the distribution of neuronal activation and the type of neurons activated by ESCS and ISMS. The first step was to establish the needed immunohistochemical (IHC) staining protocols. Domestic pigs were divided into naïve (n=2) and positive control (n=1) groups. The naïve control animals were anesthetized for 5 hrs. The positive control animal was anesthetized for 2 hrs and injected with hypertonic saline in hindlimb muscles. The animals were then euthanized, and the spinal cord removed for IHC analysis. Antibodies against cFos, a maker of neuronal activation, and NeuN, a neuronal marker were used. Preliminary results indicate that ESCS activates neurons in the dorsal horn with scattered activation in the intermediate and ventral regions. ISMS primarily activates neurons in the intermediate and ventral regions where locomotor-related networks reside. To the best of our knowledge, this is the first time the type and sites of activation of ESCS and ISMS are investigated. This will provide a foundational understanding of the mechanism of action of these stimulation modalities.
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来源期刊
CiteScore
3.20
自引率
3.40%
发文量
33
期刊介绍: Now in our 22nd year as the leading interdisciplinary journal of SCI rehabilitation techniques and care. TSCIR is peer-reviewed, practical, and features one key topic per issue. Published topics include: mobility, sexuality, genitourinary, functional assessment, skin care, psychosocial, high tetraplegia, physical activity, pediatric, FES, sci/tbi, electronic medicine, orthotics, secondary conditions, research, aging, legal issues, women & sci, pain, environmental effects, life care planning
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