{"title":"特定植物提取物对水果腐霉菌的体外和体内试验","authors":"Fatin Nabila Shaari, N. A. I. Mohd Zainudin","doi":"10.17576/jsm-2023-5209-06","DOIUrl":null,"url":null,"abstract":"Post-harvest losses of crops in Malaysia is estimated at around 20%. Fungal infection is one of the primary causes of post-harvest loss in fruits. The common use of synthetic fungicide to combat fruit rot fungi poses negative impacts on human health and the environment. The objective of this study was to determine the efficacy of selected plant species as a safer alternative for post-harvest treatment. Ethanolic leaf extracts of Piper sarmentosum, Psidium guajava, and Cymbopogon citratus were tested against Fusarium oxysporum from tomato rot, Fusarium proliferatum from banana rot, and Colletotrichum gloeosporioides from mango rot. In vitro analysis was conducted using poisoned food bioassay and the percentage inhibitions of Fusarium oxysporum growth were 93.50% by Piper sarmentosumextract, 46.68% by Psidium guajavaextract, and 40.48% by Cymbopogon citratus extract. A test on a different fungus, Fusarium proliferatum, showed that Piper sarmentosum, Psidium guajava, and Cymbopogon citratusextracts displayed percentage inhibitions of 88.78%, 36.66%, and 22.44%, respectively. In vitro test on Colletotrichum gloeosporioides showed that Piper sarmentosum extract inhibited the fungal growth by 96.23%, while Cymbopogon citratus and Psidium guajava extract inhibited the fungal growth by 52.62% and 52.22%, respectively. The mechanism of action of these extracts appeared to be fungistatic. Piper sarmentosum was selected for further analysis by in vivo assay as it displayed the most significant anti-fungal action. The study was conducted using Piper sarmentosumleaf extract of various concentrations (25, 50, 50, and 100 mg/mL). The leaf extract displayed dose-dependent activity, with the highest reduction in disease severity observed at concentration of 100 mg/mL. The findings showed the potential use of Piper sarmentosum, Psidium guajava, and Cymbopogon citratus leaf extract as anti-fungal agent for post-harvest treatment to minimize fruit loss. Piper sarmentosum leaf extract showed comparable inhibitory action to commercial fungicide carbendazim.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In Vitro and in Vivo Assays of Selected Plant Extracts Against Fruit Rot Fungi\",\"authors\":\"Fatin Nabila Shaari, N. A. I. Mohd Zainudin\",\"doi\":\"10.17576/jsm-2023-5209-06\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Post-harvest losses of crops in Malaysia is estimated at around 20%. Fungal infection is one of the primary causes of post-harvest loss in fruits. The common use of synthetic fungicide to combat fruit rot fungi poses negative impacts on human health and the environment. The objective of this study was to determine the efficacy of selected plant species as a safer alternative for post-harvest treatment. Ethanolic leaf extracts of Piper sarmentosum, Psidium guajava, and Cymbopogon citratus were tested against Fusarium oxysporum from tomato rot, Fusarium proliferatum from banana rot, and Colletotrichum gloeosporioides from mango rot. In vitro analysis was conducted using poisoned food bioassay and the percentage inhibitions of Fusarium oxysporum growth were 93.50% by Piper sarmentosumextract, 46.68% by Psidium guajavaextract, and 40.48% by Cymbopogon citratus extract. A test on a different fungus, Fusarium proliferatum, showed that Piper sarmentosum, Psidium guajava, and Cymbopogon citratusextracts displayed percentage inhibitions of 88.78%, 36.66%, and 22.44%, respectively. In vitro test on Colletotrichum gloeosporioides showed that Piper sarmentosum extract inhibited the fungal growth by 96.23%, while Cymbopogon citratus and Psidium guajava extract inhibited the fungal growth by 52.62% and 52.22%, respectively. The mechanism of action of these extracts appeared to be fungistatic. Piper sarmentosum was selected for further analysis by in vivo assay as it displayed the most significant anti-fungal action. The study was conducted using Piper sarmentosumleaf extract of various concentrations (25, 50, 50, and 100 mg/mL). The leaf extract displayed dose-dependent activity, with the highest reduction in disease severity observed at concentration of 100 mg/mL. The findings showed the potential use of Piper sarmentosum, Psidium guajava, and Cymbopogon citratus leaf extract as anti-fungal agent for post-harvest treatment to minimize fruit loss. 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In Vitro and in Vivo Assays of Selected Plant Extracts Against Fruit Rot Fungi
Post-harvest losses of crops in Malaysia is estimated at around 20%. Fungal infection is one of the primary causes of post-harvest loss in fruits. The common use of synthetic fungicide to combat fruit rot fungi poses negative impacts on human health and the environment. The objective of this study was to determine the efficacy of selected plant species as a safer alternative for post-harvest treatment. Ethanolic leaf extracts of Piper sarmentosum, Psidium guajava, and Cymbopogon citratus were tested against Fusarium oxysporum from tomato rot, Fusarium proliferatum from banana rot, and Colletotrichum gloeosporioides from mango rot. In vitro analysis was conducted using poisoned food bioassay and the percentage inhibitions of Fusarium oxysporum growth were 93.50% by Piper sarmentosumextract, 46.68% by Psidium guajavaextract, and 40.48% by Cymbopogon citratus extract. A test on a different fungus, Fusarium proliferatum, showed that Piper sarmentosum, Psidium guajava, and Cymbopogon citratusextracts displayed percentage inhibitions of 88.78%, 36.66%, and 22.44%, respectively. In vitro test on Colletotrichum gloeosporioides showed that Piper sarmentosum extract inhibited the fungal growth by 96.23%, while Cymbopogon citratus and Psidium guajava extract inhibited the fungal growth by 52.62% and 52.22%, respectively. The mechanism of action of these extracts appeared to be fungistatic. Piper sarmentosum was selected for further analysis by in vivo assay as it displayed the most significant anti-fungal action. The study was conducted using Piper sarmentosumleaf extract of various concentrations (25, 50, 50, and 100 mg/mL). The leaf extract displayed dose-dependent activity, with the highest reduction in disease severity observed at concentration of 100 mg/mL. The findings showed the potential use of Piper sarmentosum, Psidium guajava, and Cymbopogon citratus leaf extract as anti-fungal agent for post-harvest treatment to minimize fruit loss. Piper sarmentosum leaf extract showed comparable inhibitory action to commercial fungicide carbendazim.
期刊介绍:
Sains Malaysiana is a refereed journal committed to the advancement of scholarly knowledge and research findings of the several branches of science and technology. It contains articles on Earth Sciences, Health Sciences, Life Sciences, Mathematical Sciences and Physical Sciences. The journal publishes articles, reviews, and research notes whose content and approach are of interest to a wide range of scholars. Sains Malaysiana is published by the UKM Press an its autonomous Editorial Board are drawn from the Faculty of Science and Technology, Universiti Kebangsaan Malaysia. In addition, distinguished scholars from local and foreign universities are appointed to serve as advisory board members and referees.