Effectiveness of Four Different Obturation Techniques on the Penetration Depth of Bioceramic Sealers into the Dentin Tubules: An In Vitro Study

Introduction: We aimed to assess the penetration depth of bioceramic sealers into the dentin tubules following different root canal obturation techniques included (A) warm vertical compaction, (B) carrier-based technique, (C) cold lateral compaction, and (D) single-cone obturation. Methods: This study utilized 40 extracted lower first premolars with developed apices and circular and straight root canals. The roots were eliminated to achieve an 11-mm length with a coronal flat measurement point. ProTaper Next rotary system was used for instrumentation. For obturation procedures, gutta-percha and Bio-C bioceramic sealer were employed, and the roots were randomly divided into four study groups, including (A) warm vertical compaction, (B) carrier-based technique, (C) cold lateral compaction, and (D) single-cone obturation. Depth of sealer penetration into the tubules was assessed using scanning electron microscopy. Result: We found significant differences in the penetration depth of bioceramic sealers based on obturation techniques (p < 0.001), location of dentin tubules (coronal, middle, or apical third) (p < 0.001), and the interaction between obturation techniques and location (p = 0.042). Conclusion: The warm vertical compaction and carrier-based technique showed superior penetration depth into the dentin tubules.