印度泰米尔纳德邦韦洛尔土壤中的一种新型杀蚊细菌

P. Hemaladkshmi, K. Aneha, Bhagyashree Bora, V. Abhisubesh, Kakhuangailiu Gangmei, Sahadiya Mandodan, S. Manikandan, Jibi Lukose, A. Mathivanan, K. Vijayalakshmi, S. Poopathi
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引用次数: 0

摘要

本研究对泰米尔纳德邦韦洛尔地区不同农用土壤中的灭蚊细菌进行了深入筛选,以分离出潜在的灭蚊细菌。共从 315 个土壤样本中培养出 945 个细菌分离物,其中 3 个分离物具有杀蚊活性。我们从中选出了一种最有可能具有杀蚊活性的分离菌,并对其进行了进一步研究。通过 ilvD 引物和系统发生树,确定该分离物为苏云金芽孢杆菌(Bacillus thuringiensis israelensis)。这种新分离物对埃及伊蚊、史蒂芬斯氏疟蚊和五步蛇表现出有效的毒性。LC50 值分别为 0.757、0.52 和 0.165 毫克/升。同样,LC90 值分别为 0.963、0.794 和 0.355 毫克/升。这些结果表明,新分离物(Bti)VEVP-60 是一种很好的灭蚊候选物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Novel Mosquiocidal Bacterium from Soils of Vellore, Tamil nadu, India
In this study, intensive screening for mosquitocidal bacteria from different agricultural soils of Vellore district of Tamil Nadu was carried out to isolate potential mosquitocidal bacteria. From a total of 315 soil samples, 945 bacterial isolates were cultured, out of which three isolates showed mosquitocidal activity. One most potential mosquitocidal isolate was selected out of these and studied further. The isolate was identified by ilvD primer and phylogenetic tree as Bacillus thuringiensis israelensis. This new isolate exhibited effective toxicity against Aed. aegypti, An. stephensi, and Cx. quinquefasciatus. The LC50 values were 0.757, 0.52 and 0.165 mg/ l respectively. Similarly, the LC90 values were 0.963, 0.794 and 0.355 mg/l, respectively. These results depict that the new isolate (Bti) VEVP-60 is a good candidate for mosquito control.
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