P. Hemaladkshmi, K. Aneha, Bhagyashree Bora, V. Abhisubesh, Kakhuangailiu Gangmei, Sahadiya Mandodan, S. Manikandan, Jibi Lukose, A. Mathivanan, K. Vijayalakshmi, S. Poopathi
{"title":"印度泰米尔纳德邦韦洛尔土壤中的一种新型杀蚊细菌","authors":"P. Hemaladkshmi, K. Aneha, Bhagyashree Bora, V. Abhisubesh, Kakhuangailiu Gangmei, Sahadiya Mandodan, S. Manikandan, Jibi Lukose, A. Mathivanan, K. Vijayalakshmi, S. Poopathi","doi":"10.55446/ije.2023.1350","DOIUrl":null,"url":null,"abstract":"In this study, intensive screening for mosquitocidal bacteria from different agricultural soils of Vellore district of Tamil Nadu was carried out to isolate potential mosquitocidal bacteria. From a total of 315 soil samples, 945 bacterial isolates were cultured, out of which three isolates showed mosquitocidal activity. One most potential mosquitocidal isolate was selected out of these and studied further. The isolate was identified by ilvD primer and phylogenetic tree as Bacillus thuringiensis israelensis. This new isolate exhibited effective toxicity against Aed. aegypti, An. stephensi, and Cx. quinquefasciatus. The LC50 values were 0.757, 0.52 and 0.165 mg/ l respectively. Similarly, the LC90 values were 0.963, 0.794 and 0.355 mg/l, respectively. These results depict that the new isolate (Bti) VEVP-60 is a good candidate for mosquito control.","PeriodicalId":13463,"journal":{"name":"Indian journal of entomology","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Novel Mosquiocidal Bacterium from Soils of Vellore, Tamil nadu, India\",\"authors\":\"P. Hemaladkshmi, K. Aneha, Bhagyashree Bora, V. Abhisubesh, Kakhuangailiu Gangmei, Sahadiya Mandodan, S. Manikandan, Jibi Lukose, A. Mathivanan, K. Vijayalakshmi, S. Poopathi\",\"doi\":\"10.55446/ije.2023.1350\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In this study, intensive screening for mosquitocidal bacteria from different agricultural soils of Vellore district of Tamil Nadu was carried out to isolate potential mosquitocidal bacteria. From a total of 315 soil samples, 945 bacterial isolates were cultured, out of which three isolates showed mosquitocidal activity. One most potential mosquitocidal isolate was selected out of these and studied further. The isolate was identified by ilvD primer and phylogenetic tree as Bacillus thuringiensis israelensis. This new isolate exhibited effective toxicity against Aed. aegypti, An. stephensi, and Cx. quinquefasciatus. The LC50 values were 0.757, 0.52 and 0.165 mg/ l respectively. Similarly, the LC90 values were 0.963, 0.794 and 0.355 mg/l, respectively. These results depict that the new isolate (Bti) VEVP-60 is a good candidate for mosquito control.\",\"PeriodicalId\":13463,\"journal\":{\"name\":\"Indian journal of entomology\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-10-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indian journal of entomology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.55446/ije.2023.1350\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian journal of entomology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.55446/ije.2023.1350","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A Novel Mosquiocidal Bacterium from Soils of Vellore, Tamil nadu, India
In this study, intensive screening for mosquitocidal bacteria from different agricultural soils of Vellore district of Tamil Nadu was carried out to isolate potential mosquitocidal bacteria. From a total of 315 soil samples, 945 bacterial isolates were cultured, out of which three isolates showed mosquitocidal activity. One most potential mosquitocidal isolate was selected out of these and studied further. The isolate was identified by ilvD primer and phylogenetic tree as Bacillus thuringiensis israelensis. This new isolate exhibited effective toxicity against Aed. aegypti, An. stephensi, and Cx. quinquefasciatus. The LC50 values were 0.757, 0.52 and 0.165 mg/ l respectively. Similarly, the LC90 values were 0.963, 0.794 and 0.355 mg/l, respectively. These results depict that the new isolate (Bti) VEVP-60 is a good candidate for mosquito control.