在注射低温保存的脐带血的背景下,不同年龄的大鼠在开始非同步化过程中的血液白细胞的变化

V.V. Lomako
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引用次数: 0

摘要

有人认为,在非同步症发生之前预防性地使用低温保存的脐带血有核细胞(CBNCs)可能有助于纠正其对身体的负面影响。我们研究了 CBNCs 对幼鼠和老鼠(6 个月和 18 个月大)血液白细胞指标的功效。通过改变光照制度模拟非同步现象:光照时间增加 12 小时,光照时间为 24 小时。白细胞综合指数用于评估免疫系统的状态。非同步化导致幼鼠白细胞增多,而老龄鼠白细胞减少。带状中性粒细胞的数量在幼鼠和老龄鼠中都有所增加,分段中性粒细胞在幼鼠中减少,在老龄鼠中增加;淋巴细胞则相反;嗜酸性粒细胞在幼鼠中减少,在老龄鼠中没有变化。在患有非同步症的年轻大鼠中,年轻细胞、巨噬细胞占优势,免疫系统的体液环节被激活,自身和内源性中毒,即刻型超敏反应过程加速,身体适应性增强。在老龄大鼠中,观察到感染性中毒、免疫系统细胞环节占主导地位、过敏性和适应性降低。年轻大鼠在非同步化前注射 CBNCs 后,白细胞减少,分段中性粒细胞和嗜酸性粒细胞恢复,带状中性粒细胞增加,淋巴细胞减少。在老龄大鼠中,白细胞总数、单核细胞和嗜酸性粒细胞恢复,但淋巴细胞减少症增加。无论年龄大小,非特异性保护细胞都占主导地位;免疫系统的细胞环节被激活,适应能力下降,并表现出自体中毒。在年轻大鼠中,年轻形态的中性粒细胞增加,过敏性和免疫反应性降低,而在老年大鼠中,免疫系统与巨噬细胞的情感联系被激活。因此,在老龄大鼠非同步化开始前预防性使用人 CBNCs 后,白细胞总含量、嗜酸性粒细胞和单核细胞数量均得到恢复。在 6 个月大的大鼠中,幼型中性粒细胞的含量增加,表明白细胞生成受到刺激。两组大鼠的过敏指数均有所下降。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
BLOOD LEUKOCYTES IN RATS OF DIFFERENT AGES UNDER DESYNCHRONOSIS INITIATION AGAINST THE BACKGROUND OF CRYOPRESERVED CORD BLOOD INJECTION
It has been suggested that the preventive administration of cryopreserved cord blood nucleated cells (CBNCs) prior to the onset of desynchronosis may help to correct its negative effects on the body. The efficacy of CBNCs on blood leukocyte indicators in young and old rats (6 and 18 months old) was investigated. Desynchronosis was modelled by shifting the light regime: the duration of the light period was increased by 12 h, resulting in a light period of 24 h. Leukocyte types were determined in blood smears. Integral leukocyte indices were used to assess the state of the immune system. Desynchronosis caused leukocytosis in young rats and leukopenia in aged rats. The number of banded neutrophils increased in both, segmented neutrophils decreased in young rats and increased in aged rats; lymphocytes changed on the contrary; eosinophils decreased in young rats but did not change in aged rats. In young rats with desynchronosis, the predominance of young cells, macrophages, activation of the humoral link of the immune system, auto- and endogenous intoxication, acceleration of hypersensitivity of the immediate type processes, and increased body adaptation were noted. In aged rats, infectious intoxication, cellular link of immune system predominance, and a decrease in allergy and adaptation were observed. After CBNCs injection before desynchronosis, leukocytosis remained, segmented neutrophils and eosinophils recovered, banded neutrophils increased and lymphocytes decreased in young rats. In old rats, the total number of leukocytes, monocytes and eosinophils recovered, but lymphopenia increased. Regardless of age, the cells of non-specific protection predominated; the cellular link of the immune system activated, adaptation decreased and autointoxication was manifested. In young rats, young forms of neutrophils increased, allergy and immunoreactivity decreased, and the affective link of the immune system and macrophage in aged rats was activated. Therefore, after the preventive use of human CBNCs before desynchronosis initiation in aged rats, the total leukocyte content and the number of eosinophils and monocytes were restored. In 6-month-old rats, the content of young forms of neutrophils increased, indicating stimulation of leukopoiesis. The allergy index decreased in both groups.
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