通过sts标记鉴定小麦品种中的叶锈病基因

Pub Date : 2023-10-12 DOI:10.3329/bjb.v52i3.68881

M. S. Iqbal, Wesal Ahmad, M. Akbar, Inamullah

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引用次数: 0

摘要

由三尖杉菌引起的叶锈病是造成小麦减产的最具破坏性的病害。通过 STS 标记筛选了 38 个小麦品种的 4 个叶锈病抗性基因，即 Lr10、Lr26、Lr34 和 Lr47。其中，16 个基因型含有 Lr10 基因，8 个基因型含有 Lr26 基因，10 个基因型同时含有 Lr34 和 Lr47 基因。品种 Anza+2ns 因含有三个 Lr 基因（Lr10、Lr26 和 Lr47）而显示出良好的结果，值得推荐用于作物改良和/或直接用于农民田间。Chakwal-97、Indus-79、Marwat-Y-01、Mumal-2002、Khyber-83、Pasban-90、Pari-73、Lr-268 和 Satluge-86 有 2 个基因，23 个有单基因，5 个无任何反应。扩增出的 310、258、1000 和 380-450 bps 大小的诊断带显示存在 Lr10、Lr34、Lr26 和 Lr47。建议在标记辅助育种过程中对标记 STS 进行大规模种质筛选。孟加拉国植物学杂志52(3):709-714，2023 年（9 月）

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Identification of leaf rust genes in wheat varieties through sts markers

Leaf rust caused by Puccinia triticinia is the most destructive disease which reduces wheat production. Thirty-eight wheat varieties were screened for four leaf rust resistance genes viz. Lr10, Lr26, Lr34 and Lr47 through STS markers. Among them Lr10 gene showed its presence in 16 genotypes, Lr26 in 8, both Lr34 and Lr47 in 10 each. Variety Anza+2ns showed promising results by the presence of three Lr genes (Lr10, Lr26 and Lr47) surely recommended for crop improvement and/or direct utility at farmers field. Chakwal-97, Indus-79, Marwat-Y-01, Mumal-2002, Khyber-83, Pasban-90, Pari-73, Lr-268, and Satluge-86 showed 2 genes presence while 23 with single gene and 5 without any response. A diagnostic band of size 310, 258, 1000 and 380-450 bps was amplified showing presence of Lr10, Lr34, Lr26 and Lr47. Marker STS are suggested for large scale germplasm screening during marker assisted breeding. Bangladesh J. Bot. 52(3): 709-714, 2023 (September)

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