有机和水提取物的抗菌活性、细胞毒性和植物化学分析

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摘要

在抗菌药耐药性不断上升的时代,传统医学实践系统利用植物进行治疗。目前的研究调查了皮里沙棘的抗菌活性,皮里沙棘的块茎可用于治疗疾病。目前关于皮里沙棘的功效、安全性和化学成分的研究十分有限。本研究的目的是调查其抗菌活性、细胞毒性和植物化学成分。粉末材料通过水和有机溶剂浸泡提取,有机溶剂依次为石油醚(100%)、二氯甲烷(100%)、二氯甲烷:甲醇(1:1)、甲醇(100%)和水。采用盘扩散法研究 100 mg/ml 的抗菌活性,并确定最低抑菌浓度。使用盐水虾致死试验评估了提取物的细胞毒性。植物化学筛选采用标准程序进行。甲醇:二氯甲烷(1:1)萃取物对金黄色葡萄球菌的最大抑菌区为 18.0 ± 0.0 mm,而水萃取物对白色念珠菌的抑菌区为 10.0 ± 0.0 mm。活性二氯甲烷:甲醇(1:1)对蜡样芽孢杆菌、铜绿假单胞菌和金黄色葡萄球菌的最小抑菌浓度最低。获得的最小抑菌浓度值分别为18.75 + 00、14.06 + 4.7 和 28.13 + 9.4 毫克/毫升。最低杀菌浓度值分别为分别为 62.5 + 12.5、20.31 + 9.5 和 40.63 + 19 毫克/毫升。石油醚和二氯甲烷提取物对盐水虾的细胞毒性最高,半数致死浓度为 55.0 微克/毫升,而水提取物则无毒。检测到了生物碱、萜类化合物、氨基酸、碳水化合物、黄酮类化合物、皂甙、单宁、强心甙、二萜、黄原蛋白、花青素、香豆素和草酸盐。结论是皮里沙棘的提取物具有抗菌活性,水提取物是安全的。因此,需要分离出具有抗菌活性的次生代谢物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antimicrobial activity, cytotoxicity, and phytochemical assays of organic and aqueous extracts from Sarcophyte piriei
Traditional medicine practice systems employ plants in the era of rising antimicrobial resistance. The current research investigates the antimicrobial activities of Sarcophyte piriei, whose tuber is utilized to manage diseases. There are limited studies on the efficacy, safety, and chemical composition of the Sarcophyte piriei. The objective was to investigate the antimicrobial activity, cytotoxicity, and phytochemical components. The powdered material was extracted by maceration using water and organic solvents progressively from petroleum ether (100%), dichloromethane (100%), dichloromethane: methanol (1:1) and methanol (100%) and water. Disk diffusion method was used to investigate antimicrobial activity at 100 mg/ml and minimum inhibitory concentration determined. Cytotoxicity of the extracts were evaluated using brine shrimp lethality assay. Phytochemical screening was done using standard procedures. The extracts revealed varied antimicrobial effects, the methanol: dichloromethane (1:1) extracts revealed the highest zone of inhibition 18.0 ± 0.0 mm against S. aureus while aqueous had a zone of 10.0 ± 0.0 mm against C. albicans. The lowest minimum inhibitory concentration of the active dichloromethane: methanol (1:1) against Bacillus cereus, Pseudomonas aeruginosa and Staphylococcus aureus. Minimum inhibitory concentration values obtained were: 18.75 + 00, 14.06 + 4.7 and 28. 13 + 9.4 mg/ml respectively. The minimum bactericidal concentrations values were: 62.5 + 12.5, 20.31 + 9.5 and 40.63 + 19 mg/ml respectively. The petroleum ether and dichloromethane extracts had the highest cytotoxic effects against brine shrimp with LC50 value of 55.0 µg/ml while the aqueous extract was nontoxic. Alkaloids, terpenoids, amino acids, carbohydrates, flavonoids, saponins, tannins, cardiac glycosides, diterpenes, xanthoproteins, anthocyanins, coumarins and oxalates were detected. It was concluded that the extracts of Sarcophyte piriei has antimicrobial activity and the water extracts is safe. Therefore, the secondary metabolites responsible for antimicrobial activity require to be isolated.
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