trueperi Halobacillus CT7:一种从 Çankırı 盐矿分离出来的孢子形成型、产明胶酶、耐盐细菌

Fevziye Işıl Kesbiç, N. Gültepe, N. Tüzemen
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摘要

嗜卤微生物是一类能在高浓度盐环境中传播的极端生物,其中很大一部分是嗜卤细菌。盐矿是检测到嗜卤细菌的重要来源。本研究从 Çankırı 盐矿中分离出了嗜盐细菌 Halobacillus trueperi CT7。通过 DNA 分离、序列分析和生化分析,确定该菌株与 trueperi 卤杆菌的相似度为 98.16%。此外,还对 trueperi 卤杆菌进行了二维(扫描电子显微镜)和三维(原子力显微镜)成像。为了确定该微生物的工业应用潜力,定性地确定了该物种可生长的最低和最高盐浓度、温度和 pH 值范围以及酶活性。人们对将嗜卤生物用于极端工业过程的兴趣与日俱增。我们认为这项研究将有助于今后对嗜卤细菌的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Halobacillus trueperi CT7: A spore-forming, gelatinase producing, salt-tolerant bacteria isolated from Çankırı Salt Mine
Halophilic microorganisms are an extreme group of organisms that can spread at high salt concentrations, and a significant part of them consists of halophilic bacteria. Salt mines are important sources where halophilic bacteria are detected. In this study, Halobacillus trueperi CT7, a halophilic bacterium, was isolated from Çankırı Salt Mine. It was determined that this strain obtained showed 98.16 % similarity to Halobacillus trueperi by DNA isolation and sequence analysis as well as biochemical analysis. In addition, two-dimensional (scanning electron microscopy) and three-dimensional (atomic force microscopy) images of Halobacillus trueperi were performed. In order to determine the industrial use potential of the microorganism, the minimum and maximum salt concentrations, temperature and pH ranges, as well as the enzyme activities, where the species can grow, were determined qualitatively. The interest in halophilic organisms for their use in extreme industrial processes is increasing day by day. It is thought that this study will contribute to future studies on halophilic bacteria.
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