L. V. Kravchenko, M. Levkovich, S. B. Berezhanskaya, A. A. Afonin, I. Krukier, O. Puzikova, I. V. Panova, D. Sozaeva, V. Popova, A. Moskovkina, N. Drukker
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Typing of lymphocytes was carried out using monoclonal antibodies to differentiation clusters CD3+, CD3+CD69+, CD3+CD71+, CD3+CD95+ from Immunotech (France). The observation groups consisted of 30 children (41.1%) with frequent acute respiratory infections (4—5 episodes per year) in the first year of life and 43 people (58.9%) — children with no acute respiratory infection’sepisodes in the first-year life. Results. From the set of studied T-lymphocytes, statistically significant for the prognosis of frequent acute respiratory infections in the first year of life in children with cerebral ischemia who underwent cytomegalovirus infection in the neonatal period were found: CD3+ CD71+, CD3+ CD95+. 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引用次数: 0
摘要
目的:制定新生儿期感染过巨细胞病毒的脑缺血患儿出生后第一年呼吸道常见疾病的预后标准。研究方法:选取新生儿期感染过巨细胞病毒的 73 名脑缺血患儿作为研究对象。所有被观察的患者在三个月大时都接受了外周血 T 淋巴细胞群体组成研究,使用流式细胞仪检测膜标记物的表达,同时考虑贝克曼库尔特 Epics XL II 激光流式细胞仪的检测结果。使用法国 Immunotech 公司生产的分化群 CD3+、CD3+CD69+、CD3+CD71+、CD3+CD95+ 单克隆抗体对淋巴细胞进行分型。观察组中有30名儿童(41.1%)在出生后第一年经常出现急性呼吸道感染(每年4-5次),另有43名儿童(58.9%)在出生后第一年没有出现急性呼吸道感染。研究结果从研究的一组 T 淋巴细胞中发现,在新生儿期感染过巨细胞病毒的脑缺血患儿在出生后第一年频繁发生急性呼吸道感染的预后具有统计学意义:CD3+ CD71+、CD3+ CD95+。研究发现,3 个月大时血清中 CD3+ CD71+ 水平降低、CD3+ CD95+ 水平升高的患儿,在出生后第一年会频繁发生急性呼吸道感染。
Prediction of frequent acute respiratory infections in the first year of life in children with cerebral ischemia, survivors of cytomegalovirus infection in the neonatal period
Objective: to develop prognostic criteria for frequent respiratory diseases in the first year of life in children with cerebral ischemia who had a cytomegalovirus infection in the neonatal period. Research methods: 73 children of the first year of life with cerebral ischemia, who underwent cytomegalovirus infection in the neonatal period, were deployed. All observed patients at the age of three months underwent a study of the population composition of peripheral blood T-lymphocytes using flow cytometry for the expression of membrane markers, taking into account the results on a Beckman Coulter Epics XL II laser flow cytometer. Typing of lymphocytes was carried out using monoclonal antibodies to differentiation clusters CD3+, CD3+CD69+, CD3+CD71+, CD3+CD95+ from Immunotech (France). The observation groups consisted of 30 children (41.1%) with frequent acute respiratory infections (4—5 episodes per year) in the first year of life and 43 people (58.9%) — children with no acute respiratory infection’sepisodes in the first-year life. Results. From the set of studied T-lymphocytes, statistically significant for the prognosis of frequent acute respiratory infections in the first year of life in children with cerebral ischemia who underwent cytomegalovirus infection in the neonatal period were found: CD3+ CD71+, CD3+ CD95+. It was revealed that in children with a reduced level of CD3+ CD71+ and an increased level of CD3+ CD95+ in blood serum at the age of 3 months, frequent acute respiratory infections occurred in the first year of life.