{"title":"病因不明的猫小肠结肠炎患者红细胞、血红蛋白和红细胞指数的变化","authors":"L. Karpenko, A. Bakhta, P. Pogodaeva, P. Bokhan","doi":"10.31043/2410-2733-2023-3-110-113","DOIUrl":null,"url":null,"abstract":"Purpose: Study red blood parameters in a group of cats with enterocolitis of unknown etiology using standard hematological research methods.Materials and methods. During the study, standard methods of hematological analysis were used: erythrocyte counting was performed in a Goryaev chamber using whole blood diluted with isotonic sodium chloride solution 200 times, counting was carried out using a light-optical microscope Miromed-2 var. 3-20 inf. lens x8, eyepiece x10; the amount of hemoglobin was determined by the colorimetric method, diluting whole blood 200 times with a solution of 0,04 % ammonia on a Microlab-540 device; hematocrit value was determined by centrifugation using a Vilitek DSC-100MH-2 hematocrit centrifuge; the reaction to the erythrocyte sedimentation rate was performed in Panchenkov pipettes; erythrocyte indices and blood color index were calculated using generally accepted formulas.Results. Thanks to the data obtained, we concluded that disruption of enzymatic processes, deterioration of absorption in the intestine and imbalance of symbiont microflora caused by enterocolitis can lead to the development of anemic conditions, manifested by changes in red blood parameters.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"61 4","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Changes of erythrocytes, hemoglobin and erythrocyte indices in cats with enterocolitis of unknown etiology\",\"authors\":\"L. Karpenko, A. Bakhta, P. Pogodaeva, P. Bokhan\",\"doi\":\"10.31043/2410-2733-2023-3-110-113\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Purpose: Study red blood parameters in a group of cats with enterocolitis of unknown etiology using standard hematological research methods.Materials and methods. During the study, standard methods of hematological analysis were used: erythrocyte counting was performed in a Goryaev chamber using whole blood diluted with isotonic sodium chloride solution 200 times, counting was carried out using a light-optical microscope Miromed-2 var. 3-20 inf. lens x8, eyepiece x10; the amount of hemoglobin was determined by the colorimetric method, diluting whole blood 200 times with a solution of 0,04 % ammonia on a Microlab-540 device; hematocrit value was determined by centrifugation using a Vilitek DSC-100MH-2 hematocrit centrifuge; the reaction to the erythrocyte sedimentation rate was performed in Panchenkov pipettes; erythrocyte indices and blood color index were calculated using generally accepted formulas.Results. Thanks to the data obtained, we concluded that disruption of enzymatic processes, deterioration of absorption in the intestine and imbalance of symbiont microflora caused by enterocolitis can lead to the development of anemic conditions, manifested by changes in red blood parameters.\",\"PeriodicalId\":346303,\"journal\":{\"name\":\"Genetics and breeding of animals\",\"volume\":\"61 4\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-11-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genetics and breeding of animals\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.31043/2410-2733-2023-3-110-113\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetics and breeding of animals","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31043/2410-2733-2023-3-110-113","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Changes of erythrocytes, hemoglobin and erythrocyte indices in cats with enterocolitis of unknown etiology
Purpose: Study red blood parameters in a group of cats with enterocolitis of unknown etiology using standard hematological research methods.Materials and methods. During the study, standard methods of hematological analysis were used: erythrocyte counting was performed in a Goryaev chamber using whole blood diluted with isotonic sodium chloride solution 200 times, counting was carried out using a light-optical microscope Miromed-2 var. 3-20 inf. lens x8, eyepiece x10; the amount of hemoglobin was determined by the colorimetric method, diluting whole blood 200 times with a solution of 0,04 % ammonia on a Microlab-540 device; hematocrit value was determined by centrifugation using a Vilitek DSC-100MH-2 hematocrit centrifuge; the reaction to the erythrocyte sedimentation rate was performed in Panchenkov pipettes; erythrocyte indices and blood color index were calculated using generally accepted formulas.Results. Thanks to the data obtained, we concluded that disruption of enzymatic processes, deterioration of absorption in the intestine and imbalance of symbiont microflora caused by enterocolitis can lead to the development of anemic conditions, manifested by changes in red blood parameters.