治疗蛋白质下游纯化的挑战和解决方案

Q2 Medicine

Antibody Therapeutics Pub Date : 2023-11-19 DOI:10.1093/abt/tbad028

Shuo Tang, Jiaoli Tao, Ying Li

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引用次数: 0

摘要

重组蛋白技术的创新为这些治疗蛋白的纯化带来了一系列挑战。本文深入探讨了为人工设计的治疗蛋白开发纯化工艺的复杂情况。关键的障碍包括控制蛋白质的减少、蛋白质的捕获、确保稳定性、消除聚集、去除宿主细胞蛋白质 (HCP) 以及优化蛋白质的回收。在这篇综述中，我们概述了获得高纯度产品的纯化策略，强调了规避重组治疗蛋白所带来的独特挑战的相应解决方案，并通过案例研究举例说明了实际应用。最后，对未来纯化工艺的发展提出了展望。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Challenges and solutions for the downstream purification of therapeutic proteins

The innovation in recombinant protein technology has brought forth a host of challenges related to the purification of these therapeutic proteins. This article delves into the intricate landscape of developing purification processes for artificially designed therapeutic proteins. The key hurdles include controlling protein reduction, protein capture, ensuring stability, eliminating aggregates, removing host cell proteins (HCPs), and optimizing protein recovery. In this review, we outline the purification strategies in order to obtain products of high purity, highlighting the corresponding solutions to circumvent the unique challenges presented by recombinant therapeutic proteins, and exemplify the practical applications by case studies. Finally, a perspective towards future purification process development is provided.

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来源期刊

Antibody Therapeutics Medicine-Immunology and Allergy

CiteScore

8.70

自引率

0.00%

发文量

审稿时长

8 weeks