Ni Putu Kasturiasih, Ahyar Ahmad, R. Arfah, Siti Khairunnur, N. Soekamto, Paulina Taba, Y. Hala, H. Karim
{"title":"绿色藻类 Caulerpa lentillifera 的藻溶弧菌细菌共生体 L-天冬酰胺酶的生产、特征和毒性试验","authors":"Ni Putu Kasturiasih, Ahyar Ahmad, R. Arfah, Siti Khairunnur, N. Soekamto, Paulina Taba, Y. Hala, H. Karim","doi":"10.20884/1.jm.2023.18.3.8153","DOIUrl":null,"url":null,"abstract":"L-asparaginase is an enzyme that can be used as an anticancer therapeutic agent. This study aimed to optimize fermentation time for production, optimization, and to test the toxicity of L-asparaginase from Vibrio alginolyticus (V. alginolyticus) bacterial symbiont of green algae Caulerpa lentillifera (C. lentillifera). The activity of L-asparaginase enzyme assay and the toxicity test were done by using the Nessler method and the Brine Shrimp Lethality Test (BSLT) method respectively. The results show L-asparaginase with 66 hours of fermentation time indicates the highest enzyme activity. L-asparaginase mentioned has an optimum enzyme activity of 17.99 U/mL for 30 minutes of incubation time at 37 °C, and pH 7.5. The BSLT results show LC50 value of 17.83 μg/mL indicating the enzyme is bio-toxic with high-level toxicity, which can continue for cytotoxicity tests on cancer cells.","PeriodicalId":18773,"journal":{"name":"Molekul","volume":"11 9","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Production, Characterization, and Toxicity Test of L-asparaginase from Vibrio alginolyticus Bacterial Symbiont of Green Algae Caulerpa lentillifera\",\"authors\":\"Ni Putu Kasturiasih, Ahyar Ahmad, R. Arfah, Siti Khairunnur, N. Soekamto, Paulina Taba, Y. Hala, H. Karim\",\"doi\":\"10.20884/1.jm.2023.18.3.8153\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"L-asparaginase is an enzyme that can be used as an anticancer therapeutic agent. This study aimed to optimize fermentation time for production, optimization, and to test the toxicity of L-asparaginase from Vibrio alginolyticus (V. alginolyticus) bacterial symbiont of green algae Caulerpa lentillifera (C. lentillifera). The activity of L-asparaginase enzyme assay and the toxicity test were done by using the Nessler method and the Brine Shrimp Lethality Test (BSLT) method respectively. The results show L-asparaginase with 66 hours of fermentation time indicates the highest enzyme activity. L-asparaginase mentioned has an optimum enzyme activity of 17.99 U/mL for 30 minutes of incubation time at 37 °C, and pH 7.5. The BSLT results show LC50 value of 17.83 μg/mL indicating the enzyme is bio-toxic with high-level toxicity, which can continue for cytotoxicity tests on cancer cells.\",\"PeriodicalId\":18773,\"journal\":{\"name\":\"Molekul\",\"volume\":\"11 9\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-11-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molekul\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.20884/1.jm.2023.18.3.8153\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Chemistry\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molekul","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.20884/1.jm.2023.18.3.8153","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Chemistry","Score":null,"Total":0}
Production, Characterization, and Toxicity Test of L-asparaginase from Vibrio alginolyticus Bacterial Symbiont of Green Algae Caulerpa lentillifera
L-asparaginase is an enzyme that can be used as an anticancer therapeutic agent. This study aimed to optimize fermentation time for production, optimization, and to test the toxicity of L-asparaginase from Vibrio alginolyticus (V. alginolyticus) bacterial symbiont of green algae Caulerpa lentillifera (C. lentillifera). The activity of L-asparaginase enzyme assay and the toxicity test were done by using the Nessler method and the Brine Shrimp Lethality Test (BSLT) method respectively. The results show L-asparaginase with 66 hours of fermentation time indicates the highest enzyme activity. L-asparaginase mentioned has an optimum enzyme activity of 17.99 U/mL for 30 minutes of incubation time at 37 °C, and pH 7.5. The BSLT results show LC50 value of 17.83 μg/mL indicating the enzyme is bio-toxic with high-level toxicity, which can continue for cytotoxicity tests on cancer cells.
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