从尼日利亚食品样品中分离出的植物乳杆菌菌株 y11(MW642245.1)中提取的植物皂苷 Y11 的生产和特性及其在牛奶防腐中的应用

A.A. Adegoke, U.C. Ben, O.K. Fatunla
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摘要

采用传统方法和综合方法对乳酸菌(LAB)中的细菌素进行了表征,并对其进行了生物保存试验。通过生化鉴定,从 12 种不同的发酵/非发酵食品中分离出的细菌菌株中,约有 66.7%(n=180)是潜在的 LAB 菌株。经 16S rRNA 测序确认,选出了产菌量最高的菌株,并将其作为植物乳杆菌菌株 Y11(登录号:MW642245.1)提交至美国国家生物技术信息中心 GenBank。聚合酶链式反应(PCR)显示,植物乳杆菌 Y11 株含有毒力基因(细胞毒素 K 和溶血素 C)。产生的细菌素被标记为植物霉素 Y11,分子量为 1540 Da,氨基酸序列为 NSHGTADYCVMWLIXK,具有广泛的 pH 稳定性(2-10)、高热稳定性和对蛋白酶敏感性等优点。该细菌素对细菌参考菌株具有广谱活性,细菌素活性范围为 3200Au/mL 至 6400Au/mL。与苯甲酸钠相比,细菌素在牛奶中的防腐能力更强,与对照组相比,可抑制细菌和真菌的数量。经植物菌素 Y11 和苯甲酸钠处理的牛奶中,需氧中嗜酸性细菌数量的对数值分别减少了 8.02±0.15 和 8.43±0.21 Log 10 CFU/mL。经植物菌素 Y11 和苯甲酸钠处理的牛奶中真菌数量的对数值降幅分别为 ≥4.41±0.11 和 ≥3.1±0.2 Log 10 CFU/mL。植物霉素 Y11 在细菌毒素分类计划中被归为 IIa 类,它能维持牛奶的某些感官条件,因此有望成为一种安全的生物防腐剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Production and characterization of plantaricin Y11 from Lactiplantibacillus Plantarum strain y11 (MW642245.1) isolated from Nigerian food samples and their applications in preserving milk
Bacteriocin from lactic acid bacterium (LAB) was characterized and its biopreservation assay carried out using conventional  methods and integrative approaches. About 66.7% (n=180) of the bacterial strains isolated from twelve different  fermented/non-fermented food products were potential LAB strains by biochemical characterization. The  highest bacteriocin  producing strain was selected and confirmed with 16S rRNA sequencing and submitted to GenBank  of National Center for  Biotechnology Information as Lactiplantibacillus plantarum strain Y11 (Accession number:  MW642245.1). Polymerase  Chain Reaction (PCR) revealed the presence of virulence genes (cytotoxin K and haemolysin  C) in L. plantarum Y11. The  bacteriocin produced was labelled as plantaricin Y11 with molecular weight of 1540 Da,  amino acid sequence was  NSHGTADYCVMWLIXK, and possessed the merits of wide pH stability (2–10), high thermal  stability and sensitivity to  protease. The bacteriocin showed wide spectrum of activity against bacterial reference strains  with bacteriocin activity  ranging from 3200Au/mL to 6400Au/mL. Preservative potentials of the bacteriocin  compared favourably with sodium benzoate in milk, inhibiting bacterial and fungal load compared to the control. There  was log reduction of aerobic mesophilic bacterial count of 8.02±0.15 and 8.43±0.21 Log 10 CFU/mL in milk treated with  plantaricin Y11 and sodium benzoate,  respectively. The log reductions of fungal count in plantaricin Y11 and sodium  benzoate treated milk ≥4.41±0.11 and  ≥3.1±0.2 Log 10 CFU/mL respectively. The plantaricin Y11 was then classified as  class IIa in bacteriocin classification  schemes and it sustained some organoleptic conditions of milk making it a  promising candidate for safe bio-preservatives.
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