{"title":"为增材制造中的水凝胶打印制备生物墨水","authors":"Abernice Ann Abert, Masni-Azian Akiah","doi":"10.37934/mjcsm.12.1.4350","DOIUrl":null,"url":null,"abstract":"Three-dimensional (3D) bioprinting is a significant advancement in tissue engineering as it enables the printing of relevant scaffolds used for tissue repair and treating conditions like organ failure. However, printing soft biomaterials has been a major challenge due to their susceptibility to gravitational collapse. A GelPrint Scaffold method for hydrogel preparation was developed to overcome the obstacle. It provides a solution by holding the soft biomaterials in a gelatin slurry support bath during printing, polymerizing the biomaterial for crosslinking for a well-structured scaffold build-up. The bioprinting process of the alginate scaffold was conducted using a customized liquid extruder attached to a commercial 3D printer. The pores of the crosslinked printed structures were measured to identify the susceptibility of the scaffold for cell culture. The results demonstrated that the approach successfully fabricated 3D printed alginate scaffolds, approximately 90% similar to the Computer-aided design (CAD) design dimension, which has the potential to be applied to various tissue engineering applications. These scaffolds hold great promise for various tissue engineering applications, indicating the potential of the adapted bioink preparation method in advancing the field of regenerative medicine.","PeriodicalId":419270,"journal":{"name":"Malaysian Journal on Composites Science and Manufacturing","volume":"20 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Preparation of Bioink for Hydrogel Printing in Additive Manufacturing\",\"authors\":\"Abernice Ann Abert, Masni-Azian Akiah\",\"doi\":\"10.37934/mjcsm.12.1.4350\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Three-dimensional (3D) bioprinting is a significant advancement in tissue engineering as it enables the printing of relevant scaffolds used for tissue repair and treating conditions like organ failure. However, printing soft biomaterials has been a major challenge due to their susceptibility to gravitational collapse. A GelPrint Scaffold method for hydrogel preparation was developed to overcome the obstacle. It provides a solution by holding the soft biomaterials in a gelatin slurry support bath during printing, polymerizing the biomaterial for crosslinking for a well-structured scaffold build-up. The bioprinting process of the alginate scaffold was conducted using a customized liquid extruder attached to a commercial 3D printer. The pores of the crosslinked printed structures were measured to identify the susceptibility of the scaffold for cell culture. The results demonstrated that the approach successfully fabricated 3D printed alginate scaffolds, approximately 90% similar to the Computer-aided design (CAD) design dimension, which has the potential to be applied to various tissue engineering applications. These scaffolds hold great promise for various tissue engineering applications, indicating the potential of the adapted bioink preparation method in advancing the field of regenerative medicine.\",\"PeriodicalId\":419270,\"journal\":{\"name\":\"Malaysian Journal on Composites Science and Manufacturing\",\"volume\":\"20 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-11-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Malaysian Journal on Composites Science and Manufacturing\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.37934/mjcsm.12.1.4350\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Malaysian Journal on Composites Science and Manufacturing","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.37934/mjcsm.12.1.4350","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
三维(3D)生物打印技术是组织工程学的一大进步,因为它可以打印出用于组织修复和治疗器官衰竭等疾病的相关支架。然而,由于软质生物材料易受重力影响而坍塌,因此打印软质生物材料一直是一项重大挑战。为了克服这一障碍,我们开发了一种用于制备水凝胶的 GelPrint 支架方法。它提供了一种解决方案,即在打印过程中将软生物材料保持在明胶浆支撑浴中,使生物材料聚合交联,从而建立结构良好的支架。藻酸盐支架的生物打印过程是使用连接到商用 3D 打印机上的定制液体挤出机进行的。对交联打印结构的孔隙进行了测量,以确定支架对细胞培养的敏感性。结果表明,该方法成功地制造出了三维打印藻酸盐支架,与计算机辅助设计(CAD)设计尺寸的相似度约为90%,具有应用于各种组织工程的潜力。这些支架在各种组织工程应用中大有可为,表明经调整的生物墨水制备方法在推进再生医学领域的发展方面具有潜力。
Preparation of Bioink for Hydrogel Printing in Additive Manufacturing
Three-dimensional (3D) bioprinting is a significant advancement in tissue engineering as it enables the printing of relevant scaffolds used for tissue repair and treating conditions like organ failure. However, printing soft biomaterials has been a major challenge due to their susceptibility to gravitational collapse. A GelPrint Scaffold method for hydrogel preparation was developed to overcome the obstacle. It provides a solution by holding the soft biomaterials in a gelatin slurry support bath during printing, polymerizing the biomaterial for crosslinking for a well-structured scaffold build-up. The bioprinting process of the alginate scaffold was conducted using a customized liquid extruder attached to a commercial 3D printer. The pores of the crosslinked printed structures were measured to identify the susceptibility of the scaffold for cell culture. The results demonstrated that the approach successfully fabricated 3D printed alginate scaffolds, approximately 90% similar to the Computer-aided design (CAD) design dimension, which has the potential to be applied to various tissue engineering applications. These scaffolds hold great promise for various tissue engineering applications, indicating the potential of the adapted bioink preparation method in advancing the field of regenerative medicine.