慢性髓性白血病中的自然杀伤细胞亚群及其与一些炎症细胞因子的关系

IF 0.1 Q4 HEMATOLOGY
Y. Faraj, Khalid Mahdi Salih, Abderrahim Khelif, Elaf Zuhair Hameed
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引用次数: 0

摘要

与其他恶性肿瘤一样,不同亚群的自然杀伤(NK)细胞在慢性髓性白血病(CML)中识别和溶解恶性细胞的过程中起着至关重要的作用。 本研究旨在确定新诊断的 CML 患者中的两个 NK 亚群,即细胞毒性 NK(分化簇 [CD] 16+亮)和细胞因子生成 NK(CD56+亮)。 这项研究的对象是 20 名新确诊的伊拉克慢性骨髓性白血病患者(12 男 8 女),年龄在 17-55 岁之间。除患者外,还招募了 20 名健康受试者(年龄和性别匹配)作为对照组。为了识别外周血样本中的 NK 细胞及其亚群,采用流式细胞术评估了 CD45、CD3、CD56 和 CD16 标记的表达。此外,血清中γ干扰素(IFN-γ)和白细胞介素(IL)-18的水平是通过酶联免疫吸附测定法测定的。 患者确诊时的年龄为(35.6 ± 12.2 岁),男女比例为 1.5:1。新诊断的 CML 患者血清中 IL-18 的水平(30.3 ± 6.5 pg/mL)明显高于对照组(18.3 ± 7.8 pg/mL)(P < 0.0001),而新诊断的患者血清中 IFN-γ 的水平则明显下降(P = 0.006),从对照组(109.4 ± 30.3 pg/mL)降至(89.1 ± 7.2 pg/mL)。新诊断的 CML 患者的 NK 细胞比例明显低于对照组。与对照组相比,新诊断患者的细胞毒性 NK 细胞亚群(CD16+亮)明显增加,而细胞因子产生 NK 亚群(CD56+亮)明显减少。 虽然 CML 患者初诊时细胞毒性 NK 细胞(CD16+bright)亚群的比例有所升高,但这些细胞并不能识别和攻击恶性细胞,这可能是由于它们的激活受体表达较低所致,需要进一步研究。此外,本研究结果发现,CML 患者中产生细胞因子的 NK 细胞(CD56+bright)比例较低,IFN-γ 水平较低,但 IL-18 有所升高,这表明 IL-18 可能不是刺激这些细胞的主要因素,因此需要进一步研究这些 NK 细胞亚群的活化途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Subsets of natural killer cells in chronic myeloid leukemia and their relation with some inflammatory cytokines
As in other malignancies, different subsets of natural killer (NK) cells play a crucial role in the recognition and lysing of malignant cells in chronic myeloid leukemia (CML). This study aims to identify two subsets of NK, cytotoxic (cluster of differentiation [CD] 16+bright) and cytokine-producing NK (CD56+bright) in newly diagnosed CML patients. This study is conducted on 20 newly diagnosed Iraqi patients (12 males and 8 females) with CML, in chronic phase, at the age range of 17–55 years. Along with patients, 20 healthy subjects (with matched age and gender) were enrolled to act as a control group. To identify NK cells and their subsets in peripheral blood samples, the expression of CD45, CD3, CD56, and CD16 markers was evaluated by flow cytometry technique. Furthermore, the serum level of interferon gamma (IFN-γ) and interleukin (IL)-18 was determined by the enzyme-linked immunosorbent assay technique. The age of patients at the diagnosis of disease is (35.6 ± 12.2 years) and the male: female ratio was 1.5:1. The serum level of IL-18 in newly diagnosed CML patients (30.3 ± 6.5 pg/mL) was significantly (P < 0.0001) higher than those in control group (18.3 ± 7.8 pg/mL), while the serum levels of IFN-γ in newly diagnosed patients are significantly (P = 0.006) dropped down to (89.1 ± 7.2 pg/mL from that in control group (109.4 ± 30.3 pg/mL). The percentage of NK cells in newly diagnosed CML patients is significantly lower than in control group. There is a significant elevation in the cytotoxic NK cells (CD16+bright) subset, and a significant decrease in the cytokine-producing NK subset (CD56+bright) in newly diagnosed patients when compared to those in control group. Although there is an elevation in the percentage of cytotoxic NK cells (CD16+bright) subset of CML patients at the first diagnosis, these cells are not able to recognize and attack malignant cells, which may be due to low expression of their activating receptors and needs more investigation. Furthermore, present results found a low percentage of cytokine-producing NK cells (CD56+bright) and a low level of IFN-γ in CML patients, although there is an elevation in IL-18, which indicates that IL-18 may be not the main stimulator to these cells, so activation pathway of this subset of NK cells needs further investigation.
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