采用同步糖化发酵法(ssf)和单独水解发酵法(shf)将菠萝皮葡萄糖转化为生物乙醇

Hasri Tri Maya Saragih, Jhony Hartanta Sembiring, Elfrida Ginting
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摘要

本研究旨在确定采用同时糖化发酵法(SSF)和单独水解发酵法(SHF)这两种方法从菠萝皮中提取生物乙醇的产量和特性。同时糖化和发酵法发酵菠萝皮产生的生物乙醇的产率为 63.50%,而分离水解和发酵法产生的生物乙醇的产率为 58.75%。采用同步糖化和发酵法生产的生物乙醇菠萝皮废料的物理特性密度为 0.8237 w/v,而采用分离水解和发酵法生产的生物乙醇的密度为 0.8858 w/v。此外,采用同步糖化和发酵法生产的生物乙醇的粘度为 1.05 Cp,而采用分离水解和发酵法生产的生物乙醇的粘度为 1.02 Cp。同时糖化和发酵法制备的菠萝皮生物乙醇浓度为 13%,而分离水解和发酵法制备的生物乙醇浓度为 7.92%。SHF 生物乙醇的傅立叶变换红外光谱中缺少与 CH、CH3 和 CO 相关的峰值。这些峰的缺失是由于水的比例较高。此外,SSF 法制备的生物乙醇显示出与 CH、CH3 和 CO 官能团相对应的峰值。由此可以得出结论,SSF 法得到的生物乙醇效果最佳。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CONVERSION OF PINEAPPLE PEEL GLUCOSE INTO BIOETHANOL USING SIMULTANEOUS SACCHARIFICATION AND FERMENTATION (SSF) METHOD AND SEPARATE HYDROLYSIS AND FERMENTATION (SHF) METHOD
This study aims to determine the bioethanol yield and characteristics from pineapple peel with two methods such as Simultaneous Saccharification and Fermentation (SSF) and Separate Hydrolysis and Fermentation (SHF). The percent yield of bioethanol produced from the fermentation of pineapple peel (Ananas comosus) with the Simultaneous Saccharification and Fermentation method was 63.50%, while the yield of bioethanol from the Separate Hydrolysis and Fermentation method was 58.75%. The physical characteristics of bioethanol pineapple peel waste using Simultaneous Saccharification and Fermentation method has a density of 0.8237 w/v whilst with Separate Hydrolysis and Fermentation is 0.8858 w/v. Furthermore, viscosity of bioethanol using Simultaneous Saccharification and Fermentation is 1.05 Cp whereas using Separate Hydrolysis and Fermentation is 1.02 Cp. Pineapple peel bioethanol method of Simultaneous Saccharification and Fermentation has concentration of 13% while Separate Hydrolysis and Fermentation method has concentration of 7.92%. FTIR spectra from SHF bioethanol missing the peak correlated with CH, CH3, and CO. These missing peaks is due to the high percentage of water. Furthermore, bioethanol from SSF method showed peaks corresponding to CH, CH3, and CO functional groups. It can be concluded that SSF method give bioethanol with optimum result.
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