{"title":"大戟黄酮提取物对子宫内膜异位症模型小鼠增殖因子(MMP-1、MMP-3、MMP-7)的影响","authors":"Yahya Irwanto, Teguh Wiyono, Kusuma Wardani","doi":"10.55561/ajhr.v2i3.102","DOIUrl":null,"url":null,"abstract":"Introduction: Endometriosis is an inflammatory disease influenced by estrogen characterized by ectopic growth of the endometrial stroma and glands. Matrix metalloproteinase (MMP) is a proteolytic enzyme that has an important role in the remodeling of the extracellular matrix in normal tissues but also contributes to pathologies such as tumor invasion. The therapy has many drawbacks, including being expensive and in need of herbal therapy as an alternative. Phaleria macrocarpa is a native plant of Indonesia that contains flavonoids. This study aimed to evaluate the effect of Phaleria macrocarpa flavonoid isolate on the development of apoptosis, proliferation, and angiogenesis in mice model endometriosis. Material and Methods: This research is a true experimental study with a Randomized Post-Test Only with a Control Group in the laboratory. Samples are divided into six groups, a control group and an intervention group, administered with flavonoid extract from Phaleria macrocarpa. Data analysis was carried out by using the Independent T-Test with SPSS for Windows 19.0 software. Results: Each control and intervention group consisted of 6 mice. The normality test for each variable shows p> 0.05. Administration of flavonoid in each group shows a significant decrease in MMP-1 (20.4 ± 7.74 vs 65.68 ± 10.97, p=0.000), MMP-3 (53.34 ± 9.66 vs67.47 ± 10.05, p=0.000), and MMP-7 expression (40.52 ± 5.43 vs 54.13 ± 4.08, p=0.000). Conclusion: Flavonoids from the Phaleria macrocarpa fruit extract were able to reduce the expression of MMP-1, MMP-3, and MMP-7 in mice model endometriosis.","PeriodicalId":502351,"journal":{"name":"Asian Journal of Health Research","volume":"367 4","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Effect of Flavonoid Extract from Phaleria macrocarpa to Proliferating Factors (MMP-1, MMP-3, MMP-7) in Endometriosis Mice Model\",\"authors\":\"Yahya Irwanto, Teguh Wiyono, Kusuma Wardani\",\"doi\":\"10.55561/ajhr.v2i3.102\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Introduction: Endometriosis is an inflammatory disease influenced by estrogen characterized by ectopic growth of the endometrial stroma and glands. Matrix metalloproteinase (MMP) is a proteolytic enzyme that has an important role in the remodeling of the extracellular matrix in normal tissues but also contributes to pathologies such as tumor invasion. The therapy has many drawbacks, including being expensive and in need of herbal therapy as an alternative. Phaleria macrocarpa is a native plant of Indonesia that contains flavonoids. This study aimed to evaluate the effect of Phaleria macrocarpa flavonoid isolate on the development of apoptosis, proliferation, and angiogenesis in mice model endometriosis. Material and Methods: This research is a true experimental study with a Randomized Post-Test Only with a Control Group in the laboratory. Samples are divided into six groups, a control group and an intervention group, administered with flavonoid extract from Phaleria macrocarpa. Data analysis was carried out by using the Independent T-Test with SPSS for Windows 19.0 software. Results: Each control and intervention group consisted of 6 mice. The normality test for each variable shows p> 0.05. Administration of flavonoid in each group shows a significant decrease in MMP-1 (20.4 ± 7.74 vs 65.68 ± 10.97, p=0.000), MMP-3 (53.34 ± 9.66 vs67.47 ± 10.05, p=0.000), and MMP-7 expression (40.52 ± 5.43 vs 54.13 ± 4.08, p=0.000). Conclusion: Flavonoids from the Phaleria macrocarpa fruit extract were able to reduce the expression of MMP-1, MMP-3, and MMP-7 in mice model endometriosis.\",\"PeriodicalId\":502351,\"journal\":{\"name\":\"Asian Journal of Health Research\",\"volume\":\"367 4\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-12-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Asian Journal of Health Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.55561/ajhr.v2i3.102\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Asian Journal of Health Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.55561/ajhr.v2i3.102","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
导言:子宫内膜异位症是一种受雌激素影响的炎症性疾病,其特征是子宫内膜基质和腺体的异位生长。基质金属蛋白酶(MMP)是一种蛋白水解酶,在正常组织细胞外基质的重塑过程中发挥重要作用,但也会导致肿瘤侵袭等病变。这种疗法有许多缺点,包括价格昂贵,需要草药疗法作为替代。Phaleria macrocarpa是印度尼西亚的一种本地植物,含有类黄酮。本研究旨在评估大戟黄酮分离物对子宫内膜异位症小鼠模型的凋亡、增殖和血管生成的影响。材料与方法:本研究是一项真正的实验研究,在实验室中只进行随机后测,并设对照组。样本分为六组,一组为对照组,另一组为干预组。数据分析采用 SPSS for Windows 19.0 软件的独立 T 检验法。结果对照组和干预组各由 6 只小鼠组成。各变量的正态性检验结果显示 p> 0.05。各组服用黄酮类化合物后,MMP-1(20.4 ± 7.74 vs 65.68 ± 10.97,P=0.000)、MMP-3(53.34 ± 9.66 vs 67.47 ± 10.05,P=0.000)和MMP-7(40.52 ± 5.43 vs 54.13 ± 4.08,P=0.000)的表达量均显著下降。结论大叶女贞果提取物中的黄酮类化合物能够降低子宫内膜异位症小鼠模型中MMP-1、MMP-3和MMP-7的表达。
The Effect of Flavonoid Extract from Phaleria macrocarpa to Proliferating Factors (MMP-1, MMP-3, MMP-7) in Endometriosis Mice Model
Introduction: Endometriosis is an inflammatory disease influenced by estrogen characterized by ectopic growth of the endometrial stroma and glands. Matrix metalloproteinase (MMP) is a proteolytic enzyme that has an important role in the remodeling of the extracellular matrix in normal tissues but also contributes to pathologies such as tumor invasion. The therapy has many drawbacks, including being expensive and in need of herbal therapy as an alternative. Phaleria macrocarpa is a native plant of Indonesia that contains flavonoids. This study aimed to evaluate the effect of Phaleria macrocarpa flavonoid isolate on the development of apoptosis, proliferation, and angiogenesis in mice model endometriosis. Material and Methods: This research is a true experimental study with a Randomized Post-Test Only with a Control Group in the laboratory. Samples are divided into six groups, a control group and an intervention group, administered with flavonoid extract from Phaleria macrocarpa. Data analysis was carried out by using the Independent T-Test with SPSS for Windows 19.0 software. Results: Each control and intervention group consisted of 6 mice. The normality test for each variable shows p> 0.05. Administration of flavonoid in each group shows a significant decrease in MMP-1 (20.4 ± 7.74 vs 65.68 ± 10.97, p=0.000), MMP-3 (53.34 ± 9.66 vs67.47 ± 10.05, p=0.000), and MMP-7 expression (40.52 ± 5.43 vs 54.13 ± 4.08, p=0.000). Conclusion: Flavonoids from the Phaleria macrocarpa fruit extract were able to reduce the expression of MMP-1, MMP-3, and MMP-7 in mice model endometriosis.