BACTERIA GENUS FILIFACTOR IN PATIENTS WITH PERIODONTITIS AND DIABETES MELLITUS ACCORDING TO METAGENOMIC ANALYSIS OF THE PERIODONTAL MICROBIOME

Periodontal disease is a widespread pathology, and chronic periodontitis is the most severe form of its manifestation. In recent years, the problem of this polymicrobial disease has acquired particular importance due to the possibility of developing concomitant systemic effects. Quite often, chronic periodontitis is combined with type 2 diabetes mellitus. The etiopathogenetic mechanisms that determine this association include so-called periodontal pathogenic bacteria, with the recently discovered periodontal pathogen Filifactor alocis being the least studied. The purpose of the study is identification of bacteria of genus Filifactor in the periodontal microbiome in the association of chronic periodontitis and type 2 diabetes mellitus and clarification of the mechanisms of their possible influence on associated metabolic processes based on comparative metagenomic analysis. Materials and methods..A metagenomic study of periodontal pocket microbiome samples from 28 chronic periodontitis and diabetes mellitus type 2 association patients and 22 chronic periodontitis patients, as well as the periodontal sulcus microbiome from 19 clinically healthy individuals, was conducted. To determine the taxonomic composition of the microbiome, 16S sequencing of the ribosomal RNA gene was used, and metabolic pathways involving the microbiome were predicted using the shotgun method. The results obtained made it possible to establish that the most common microorganism in the association of chronic periodontitis and type 2 diabetes mellitus were Filifactor genus bacteria. The percentage of their registration correlated with low rates of metagenomic prediction of fatty acid biosynthesis and pyrimidine metabolism in lesions. Conclusion. The frequency of occurrence of Filifactor genus bacteria in patients with the association of chronic periodontitis and type 2 diabetes mellitus was associated with negative correlations with certain features of the putative metabolic pathways of the microbiome, which included fatty acid biosynthesis and pyrimidine metabolism.