Marielle Moreau, Tanesha Naiken, Gérard Bru, Clarisse Marteau, Laurence Canaple, Lorène Gourguillon, Emmanuelle Leblanc, Elodie Oger, Audrey Le Mestr, Joel Mantelin, Isabelle Imbert, C. Nizard, A. Bulteau
{"title":"PSR™(植物小核糖核酸技术)、茶叶提取物及其主要成分对皮肤细胞线粒体功能和抗氧化特性的影响","authors":"Marielle Moreau, Tanesha Naiken, Gérard Bru, Clarisse Marteau, Laurence Canaple, Lorène Gourguillon, Emmanuelle Leblanc, Elodie Oger, Audrey Le Mestr, Joel Mantelin, Isabelle Imbert, C. Nizard, A. Bulteau","doi":"10.3390/cosmetics10060172","DOIUrl":null,"url":null,"abstract":"Objective: This study explored the impact of a black tea extract obtained through (plant small RNA) PSRTM technology, characterized by its abundance of small molecules, particularly citric acid—an antioxidant and tricarboxylic acid (TCA) cycle contributor—on mitochondrial health. The primary focus was to assess whether this extract could counteract reactive oxygen species (ROS)-induced mitochondrial alterations associated with aging, which lead to impaired mitochondrial function, reduced ATP production, and increased ROS generation. Methods: The PSRTM extraction method was employed to obtain a high content of polyphenols and small molecules, particularly citric acid. Results: In comparison with a conventional extract, the PSRTM extract demonstrated significant enhancements in aconitase activity, an ROS-sensitive enzyme in the TCA cycle, as well as basal respiration and ATP synthesis in fibroblast cells and skin biopsies. Moreover, the PSRTM extract effectively reduced ROS production by safeguarding this critical enzyme within the Krebs cycle and displayed superior capabilities in scavenging free radicals when exposed to UV-induced stress. When administered post-UV exposure, the PSRTM extract protected nuclear DNA by reducing the formation of cyclobutane pyrimidine dimers (CPDs) and promoting DNA repair mechanisms. Furthermore, the extract exhibited beneficial effects on the extracellular matrix, characterized by a reduction in matrix metalloprotease 1 (MMP1) and an increase in fibrillin 1 expression. Conclusions: These findings collectively suggest that the PSRTM extract holds promising antiaging potential, potentially functioning as a mitochondrial nutrient/protector due to its multifaceted benefits on mitochondrial function, nuclear DNA integrity, and the extracellular matrix.","PeriodicalId":10735,"journal":{"name":"Cosmetics","volume":"28 2","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Impact of PSR™ (Plant Small RNA Technology), Tea Extract, and Its Principal Components on Mitochondrial Function and Antioxidant Properties in Skin Cells\",\"authors\":\"Marielle Moreau, Tanesha Naiken, Gérard Bru, Clarisse Marteau, Laurence Canaple, Lorène Gourguillon, Emmanuelle Leblanc, Elodie Oger, Audrey Le Mestr, Joel Mantelin, Isabelle Imbert, C. Nizard, A. Bulteau\",\"doi\":\"10.3390/cosmetics10060172\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: This study explored the impact of a black tea extract obtained through (plant small RNA) PSRTM technology, characterized by its abundance of small molecules, particularly citric acid—an antioxidant and tricarboxylic acid (TCA) cycle contributor—on mitochondrial health. The primary focus was to assess whether this extract could counteract reactive oxygen species (ROS)-induced mitochondrial alterations associated with aging, which lead to impaired mitochondrial function, reduced ATP production, and increased ROS generation. Methods: The PSRTM extraction method was employed to obtain a high content of polyphenols and small molecules, particularly citric acid. Results: In comparison with a conventional extract, the PSRTM extract demonstrated significant enhancements in aconitase activity, an ROS-sensitive enzyme in the TCA cycle, as well as basal respiration and ATP synthesis in fibroblast cells and skin biopsies. Moreover, the PSRTM extract effectively reduced ROS production by safeguarding this critical enzyme within the Krebs cycle and displayed superior capabilities in scavenging free radicals when exposed to UV-induced stress. When administered post-UV exposure, the PSRTM extract protected nuclear DNA by reducing the formation of cyclobutane pyrimidine dimers (CPDs) and promoting DNA repair mechanisms. Furthermore, the extract exhibited beneficial effects on the extracellular matrix, characterized by a reduction in matrix metalloprotease 1 (MMP1) and an increase in fibrillin 1 expression. Conclusions: These findings collectively suggest that the PSRTM extract holds promising antiaging potential, potentially functioning as a mitochondrial nutrient/protector due to its multifaceted benefits on mitochondrial function, nuclear DNA integrity, and the extracellular matrix.\",\"PeriodicalId\":10735,\"journal\":{\"name\":\"Cosmetics\",\"volume\":\"28 2\",\"pages\":\"\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2023-12-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cosmetics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/cosmetics10060172\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cosmetics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/cosmetics10060172","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
研究目的本研究探讨了通过(植物小 RNA)PSRTM 技术获得的红茶提取物对线粒体健康的影响,该提取物的特点是富含小分子,尤其是柠檬酸--一种抗氧化剂和三羧酸(TCA)循环促进剂。主要重点是评估这种提取物是否能抵消活性氧(ROS)诱导的与衰老相关的线粒体改变,衰老会导致线粒体功能受损、ATP 生成减少以及 ROS 生成增加。方法:采用 PSRTM 萃取法获得高含量的多酚和小分子物质,尤其是柠檬酸。结果:与传统提取物相比,PSRTM 提取物在成纤维细胞和皮肤活检中显著提高了乌头酶(TCA 循环中对 ROS 敏感的一种酶)的活性、基础呼吸作用和 ATP 合成。此外,PSRTM 提取物通过保护克雷布斯循环中的这种关键酶,有效减少了 ROS 的产生,并在紫外线诱导的压力下显示出卓越的清除自由基的能力。紫外线照射后服用 PSRTM 提取物,可减少环丁烷嘧啶二聚体(CPD)的形成,促进 DNA 修复机制,从而保护核 DNA。此外,该提取物还对细胞外基质产生了有益的影响,其特点是减少了基质金属蛋白酶 1(MMP1),增加了纤维蛋白 1 的表达。结论这些发现共同表明,PSRTM 提取物具有抗衰老的潜力,由于它对线粒体功能、核 DNA 完整性和细胞外基质具有多方面的益处,因此有可能成为线粒体营养素/保护剂。
The Impact of PSR™ (Plant Small RNA Technology), Tea Extract, and Its Principal Components on Mitochondrial Function and Antioxidant Properties in Skin Cells
Objective: This study explored the impact of a black tea extract obtained through (plant small RNA) PSRTM technology, characterized by its abundance of small molecules, particularly citric acid—an antioxidant and tricarboxylic acid (TCA) cycle contributor—on mitochondrial health. The primary focus was to assess whether this extract could counteract reactive oxygen species (ROS)-induced mitochondrial alterations associated with aging, which lead to impaired mitochondrial function, reduced ATP production, and increased ROS generation. Methods: The PSRTM extraction method was employed to obtain a high content of polyphenols and small molecules, particularly citric acid. Results: In comparison with a conventional extract, the PSRTM extract demonstrated significant enhancements in aconitase activity, an ROS-sensitive enzyme in the TCA cycle, as well as basal respiration and ATP synthesis in fibroblast cells and skin biopsies. Moreover, the PSRTM extract effectively reduced ROS production by safeguarding this critical enzyme within the Krebs cycle and displayed superior capabilities in scavenging free radicals when exposed to UV-induced stress. When administered post-UV exposure, the PSRTM extract protected nuclear DNA by reducing the formation of cyclobutane pyrimidine dimers (CPDs) and promoting DNA repair mechanisms. Furthermore, the extract exhibited beneficial effects on the extracellular matrix, characterized by a reduction in matrix metalloprotease 1 (MMP1) and an increase in fibrillin 1 expression. Conclusions: These findings collectively suggest that the PSRTM extract holds promising antiaging potential, potentially functioning as a mitochondrial nutrient/protector due to its multifaceted benefits on mitochondrial function, nuclear DNA integrity, and the extracellular matrix.