Study of Technological Parameters and Pharmacological Activity of the Gel with Strawberry (Fragaria ananassa) Leaves with Dry Extract

Introduction. Interest in herbal preparations is increasing every year, as the variety of chemical composition and pharmacological action of plants makes it possible to discover and create drugs whose therapeutic effect is due to a complex of biologically active substances. It is important for the effective treatment of diseases to have a properly selected dosage form, which provides both ease of use and targeted use of the action of the pharmacologically active drug contained in it. Therefore, obtaining and introducing new plant extracts into medical practice can become promising in the field of creating soft dosage forms. Strawberry garden leaf extract dry (strawberry extract) is one of the potential substances for obtaining effective and safe medicines. According to the results of earlier studies conducted by the staff of the Perm State Pharmaceutical Academy of the Ministry of Health of the Russian Federation, a high anti-inflammatory activity of strawberry extract was proved, comparable to the activity of the synthetic drug Indomethacin. Studies of the chronic toxicity of the extract, allergenic, immunotoxic properties and reproductive toxicity in the framework of preclinical studies have shown the safety of the obtained extract and the possibility of using the substance for further development of medicines based on it. In this regard, the study of technological parameters and pharmacological activity of a soft dosage form based on strawberry extract is of practical interest for improving the drug supply of the population and expanding the range of medicines based on plant extracts with anti-inflammatory and antimicrobial effects.Aim. Development of a soft dosage form based on strawberry leaves of dry extract and study of its pharmacological activity.Materials and methods. For the study, gels with an active substance – strawberry leaves with dry extract (JSC "Biohimmash", Russia, shelf life – 2 years), the main components – sodium carboxymethylcellulose C75 (TU 2231-002-50277563-2000, LLC "Base chemical products "Yugreactive", Russia, 151118, term storage 3 years), methylcellulose 35 (TU 2231-107-57684455-2003, JSC "UZPH", Russia, 221218, shelf life 3 years), sodium alginate (TU 15-02-544-83, LLC "Base сhemical products "Yugreactive", Russia, 151018, shelf life 3 years), glycerol (FS.2.2.0006.15, JSC "Kupavnareaktiv", Russia, 082018, shelf life 3 years), purified water (FS FS.2.2.0020.18), obtained at the reverse osmosis unit UVOI-"MF" 1812S6 (JSC "NPK MEDIAN FILTER", Russia). Вiopharmaceutical and technological methods were used in the course of the research. Antimicrobial activity was determined according to the OFS.1.2.4.0010.15 by diffusion into agar. Anti-inflammatory activity was studied on a model of acute inflammatory edema caused by subplantar injection of 0.1 ml of 1 % aqueous solution of carrageenan into the hind paw of a rat. The obtained data were processed with the calculation of the Student's t-test for independent samples. The data were considered reliable at p < 0.05.Results and discussion. Based on technological, biopharmaceutical and pharmacological research methods, the composition of a gel from strawberry leaves with dry extract, which has antimicrobial and anti-inflammatory effects, was proposed and substantiated for the first time. The biopharmaceutical availability of the gel was determined by diffusion into agar gel. The antimicrobial activity of the gel from strawberry leaves with dry extract and its anti-inflammatory activity on the model of carrageenan edema with external application of the studied dosage forms were studied.Conclusion. The study shows the possibility of expanding the range of drugs for external use with antimicrobial and anti-inflammatory activity.