内生菌枯草芽孢杆菌对马铃薯块茎贮藏期间代谢过程的影响

L. I. Pusenkova, S. Garipova, O. Lastochkina, I. Shpirnaya, V. A. Valieva
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摘要

文章研究了马铃薯块茎种植前接种枯草芽孢杆菌对不同贮藏期新作物块茎收获后生化过程的影响。用菌株 10-4 和 26D 处理的块茎中丙二醛的含量在 1 个月和 6 个月后分别比对照组降低了 15.5% 和 35.5%,以及 14.9% 和 13.6%。用菌株 10-4 预处理过的块茎中的脯氨酸含量在两个贮藏期内都与对照组差别不大,而用菌株 26D 预处理过的块茎中的脯氨酸含量则比对照组分别低 14.7% 和 26.3%。首先,处理过的块茎中保护蛋白的活性比对照组低;然后,发现块茎中胰蛋白酶抑制剂的活性明显超过短期储存的块茎。预处理块茎的蛋白质含量最初高于对照组;随后,蛋白质含量要么与对照组(菌株 26D)持平,要么比对照组(菌株 10-4)低 15%。用菌株 10-4 预处理过的块茎在储存 1 个月和 6 个月后,抗坏血酸含量分别比对照高出 16.1% 和 17.9%,而用菌株 26D 预处理过的块茎则与对照没有差别。还原糖的含量在储存 1 个月和 6 个月后(菌株 10-4)比对照组低 39.4%,储存 6 个月后(菌株 26D)比对照组低 35.6%。使用菌株 10-4 和 26D 进行预处理对块茎长期储藏期间的主要生化参数有积极影响,起到保护作用,并能保持农产品的生物价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of endophytic bacteria Bacillus subtilison metabolic processes in potato tubers during storage
The article examines the effect produced by the preplant inoculation of potato tubers with Bacillus subtilis on biochemical processes occurring in the tubers of a new crop during the postharvest period at different storage periods. The content of malondialdehyde in tubers treated with strains 10-4 and 26D decreased by 15.5 and 35.5% and by 14.9 and 13.6% relative to the control after one and six months, respectively. The proline content in tubers pretreated with strain 10-4 differed insignificantly from the control in both storage periods, while in the case of strain 26D, it was lower than the control by 14.7 and 26.3%. First, the activity of protective proteins in treated tubers decreased relative to the control; then, the activity of trypsin inhibitors in tubers was found to significantly exceed that in tubers stored for a short time. The protein content of pretreated tubers was initially higher than that of the control; then, it was either at the same level (strain 26D) or 15% lower than the control (strain 10-4). The content of ascorbic acid in tubers pretreated with strain 10-4 exceeded that of the control by 16.1 and 17.9% after one and six months of storage, respectively, whereas in the case of strain 26D, no difference from the control was noted. The amount of reducing sugars was 39.4% lower than the control after one and six months of storage (strain 10-4) and 35.6% after six months of storage (strain 26D). The pretreatment with strains 10-4 and 26D positively affected the main biochemical parameters of tubers during long-term storage, providing a protective effect and preserving the biological value of produce.
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