Elfred Kasimo, Lisa Savitri, Mujtahid Bin Abd Kadir, Moh Alimansur, Ester Lianawati Antoro
{"title":"麝香木槿（Hibiscus sabdariffa L.）丹皮（Momordica charantia）登岸模式溪螯虾致死试验（BSLT）","authors":"Elfred Kasimo, Lisa Savitri, Mujtahid Bin Abd Kadir, Moh Alimansur, Ester Lianawati Antoro","doi":"10.47539/gk.v15i2.441","DOIUrl":null,"url":null,"abstract":"Rosela (Hibiscus sabdariffa L.) dan pare (Momordica charantia) mengandung senyawa kimia yang bermanfaat bagi kesehatan, seperti asam fenolat, tannin, flavonoid, alkaloid, saponin, tanin, glikosida, dan steroid. Penelitian ini bertujuan untuk mengetahui tingkat toksisitas ekstrak kombinasi bunga rosela (Hibiscus sabdariffa L.) dan buah pare (Momordica charantia) terhadap larva Artemia salina Leach menggunakan metode Brine Shrimp Lethality Test (BSLT). Tingkat toksisitas tersebut ditunjukkan dengan nilai LC50. Metode: Penelitian uji toksisitas kombinasi ekstrak Hibiscus sabdariffa L dan Momordica charantia terhadap larva udang (Artemia salina L.) menggunakan metode BSLT. Dalam penelitian ini, ekstrak kombinasi bunga rosela dan buah pare diuji toksisitasnya terhadap larva Artemia salina Leach dengan konsentrasi 1000 ppm, 500 ppm, 200 ppm, 100 ppm, dan 50 ppm. Pengujian dilakukan sebanyak tiga kali pada masing-masing konsentrasi. Data hasil pengujian dianalisis menggunakan analisa probit, dan log konsentrasinya dihitung untuk mengetahui nilai LC50. Hasil : Ekstrak kombinasi bunga rosela (Hibiscus sabdariffa L.) dan buah pare (Momordica charantia) memiliki nilai LC50 sebesar 241,8 ppm. Kata kunci: BSLT, Hibiscus sabdariffa L, Momordica charantia Roselle (Hibiscus sabdariffa L.) and bitter melon (Momordica charantia) contain chemical compounds that are beneficial for health, such as phenolic acids, tannins, flavonoids, alkaloids, saponins, tannins, glycosides, and steroids. This study aims to determine the level of toxicity of the combined extract of roselle flowers (Hibiscus sabdariffa L.) and bitter melon fruit (Momordica charantia) against Artemia salina Leach larvae using the Brine Shrimp Lethality Test (BSLT) method. The LC50 value indicates the level of toxicity. Process: Research the toxicity test of a combination of Hibiscus sabdariffa L. and Momordica charantia extracts against shrimp larvae (Artemia salina L.) using the BSLT method. This research tested the combined extract of roselle flowers and bitter melon fruit for toxicity against Artemia salina Leach larvae at concentrations of 1000 ppm, 500 ppm, 200 ppm, 100 ppm, and 50 ppm. The test was carried out three times at each concentration. The test result data was analyzed using probit analysis, and the concentration log was calculated to determine the LC50 value. Results: The combination extract of roselle flowers (Hibiscus sabdariffa L.) and bitter melon fruit (Momordica charantia) had an LC50 value of 241.8 ppm. Keywords: BSLT, Hibiscus sabdariffa L, Momordica charantia","PeriodicalId":269988,"journal":{"name":"GEMA KESEHATAN","volume":"57 43","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"TOKSISITAS KOMBINASI EKSTRAK ROSELA (Hibiscus sabdariffa L.) 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Dalam penelitian ini, ekstrak kombinasi bunga rosela dan buah pare diuji toksisitasnya terhadap larva Artemia salina Leach dengan konsentrasi 1000 ppm, 500 ppm, 200 ppm, 100 ppm, dan 50 ppm. Pengujian dilakukan sebanyak tiga kali pada masing-masing konsentrasi. Data hasil pengujian dianalisis menggunakan analisa probit, dan log konsentrasinya dihitung untuk mengetahui nilai LC50. Hasil : Ekstrak kombinasi bunga rosela (Hibiscus sabdariffa L.) dan buah pare (Momordica charantia) memiliki nilai LC50 sebesar 241,8 ppm. Kata kunci: BSLT, Hibiscus sabdariffa L, Momordica charantia Roselle (Hibiscus sabdariffa L.) and bitter melon (Momordica charantia) contain chemical compounds that are beneficial for health, such as phenolic acids, tannins, flavonoids, alkaloids, saponins, tannins, glycosides, and steroids. This study aims to determine the level of toxicity of the combined extract of roselle flowers (Hibiscus sabdariffa L.) and bitter melon fruit (Momordica charantia) against Artemia salina Leach larvae using the Brine Shrimp Lethality Test (BSLT) method. The LC50 value indicates the level of toxicity. Process: Research the toxicity test of a combination of Hibiscus sabdariffa L. and Momordica charantia extracts against shrimp larvae (Artemia salina L.) using the BSLT method. This research tested the combined extract of roselle flowers and bitter melon fruit for toxicity against Artemia salina Leach larvae at concentrations of 1000 ppm, 500 ppm, 200 ppm, 100 ppm, and 50 ppm. The test was carried out three times at each concentration. The test result data was analyzed using probit analysis, and the concentration log was calculated to determine the LC50 value. Results: The combination extract of roselle flowers (Hibiscus sabdariffa L.) and bitter melon fruit (Momordica charantia) had an LC50 value of 241.8 ppm. 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引用次数: 0
摘要
木槿（Hibiscus sabdariffa L.）和芒果（Momordica charantia）可产生多种生物活性成分，包括酚类、单宁、黄酮类、生物碱、皂苷、鞣质、胶原蛋白和类固醇。本研究的目的是通过使用盐水虾致死率测试（BSLT）方法，对木槿（Hibiscus sabdariffa L.）和芒柄蜡（Momordica charantia）的组合进行研究。测试结果以半数致死浓度表示。方法：将木槿和芒果与幼虫（Artemia salina L.）结合使用的方法是 BSLT。在这一研究中，萝藦科植物和芒果科植物分别以 1000 ppm、500 ppm、200 ppm、100 ppm 和 50 ppm 的浓度对鲫鱼幼虫产生影响。这些数据将在下半年的实验中公布。通过概率分析和对数计算，可得出半数致死浓度。结果：木槿（Hibiscus sabdariffa L.）和香豌豆（Momordica charantia）的混合物的半数致死浓度为 241.8 ppm。Kata kunci: BSLT, Hibiscus sabdariffa L., Momordica charantia Roselle (Hibiscus sabdariffa L.) and bitter melon (Momordica charantia) contain chemical compounds that are beneficial for health, such as phenolic acids, tannins, flavonoids, alkaloids, saponins, tannins, glycosides, and steroids.本研究旨在采用盐水虾致死试验（BSLT）方法，确定洛神花（Hibiscus sabdariffa L.）和苦瓜（Momordica charantia）的混合提取物对盐水蓟马幼虫的毒性水平。LC50 值表示毒性水平。过程：使用盐水虾致死试验（BSLT）方法，研究芙蓉和红景天提取物组合对盐水虾幼虫（Artemia salina L.）的毒性试验。这项研究测试了洛神花和苦瓜果实提取物在 1000 ppm、500 ppm、200 ppm、100 ppm 和 50 ppm 浓度下对鲤科幼虫的毒性。每个浓度的试验均进行三次。试验结果数据采用 probit 分析法进行分析，并计算浓度对数值，以确定 LC50 值。结果洛神花（Hibiscus sabdariffa L.）和苦瓜（Momordica charantia）的混合提取物的半数致死浓度为 241.8 ppm。关键词BSLT 木槿花 苦瓜果实
TOKSISITAS KOMBINASI EKSTRAK ROSELA (Hibiscus sabdariffa L.) DAN PARE (Momordica charantia) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT)
Rosela (Hibiscus sabdariffa L.) dan pare (Momordica charantia) mengandung senyawa kimia yang bermanfaat bagi kesehatan, seperti asam fenolat, tannin, flavonoid, alkaloid, saponin, tanin, glikosida, dan steroid. Penelitian ini bertujuan untuk mengetahui tingkat toksisitas ekstrak kombinasi bunga rosela (Hibiscus sabdariffa L.) dan buah pare (Momordica charantia) terhadap larva Artemia salina Leach menggunakan metode Brine Shrimp Lethality Test (BSLT). Tingkat toksisitas tersebut ditunjukkan dengan nilai LC50. Metode: Penelitian uji toksisitas kombinasi ekstrak Hibiscus sabdariffa L dan Momordica charantia terhadap larva udang (Artemia salina L.) menggunakan metode BSLT. Dalam penelitian ini, ekstrak kombinasi bunga rosela dan buah pare diuji toksisitasnya terhadap larva Artemia salina Leach dengan konsentrasi 1000 ppm, 500 ppm, 200 ppm, 100 ppm, dan 50 ppm. Pengujian dilakukan sebanyak tiga kali pada masing-masing konsentrasi. Data hasil pengujian dianalisis menggunakan analisa probit, dan log konsentrasinya dihitung untuk mengetahui nilai LC50. Hasil : Ekstrak kombinasi bunga rosela (Hibiscus sabdariffa L.) dan buah pare (Momordica charantia) memiliki nilai LC50 sebesar 241,8 ppm. Kata kunci: BSLT, Hibiscus sabdariffa L, Momordica charantia Roselle (Hibiscus sabdariffa L.) and bitter melon (Momordica charantia) contain chemical compounds that are beneficial for health, such as phenolic acids, tannins, flavonoids, alkaloids, saponins, tannins, glycosides, and steroids. This study aims to determine the level of toxicity of the combined extract of roselle flowers (Hibiscus sabdariffa L.) and bitter melon fruit (Momordica charantia) against Artemia salina Leach larvae using the Brine Shrimp Lethality Test (BSLT) method. The LC50 value indicates the level of toxicity. Process: Research the toxicity test of a combination of Hibiscus sabdariffa L. and Momordica charantia extracts against shrimp larvae (Artemia salina L.) using the BSLT method. This research tested the combined extract of roselle flowers and bitter melon fruit for toxicity against Artemia salina Leach larvae at concentrations of 1000 ppm, 500 ppm, 200 ppm, 100 ppm, and 50 ppm. The test was carried out three times at each concentration. The test result data was analyzed using probit analysis, and the concentration log was calculated to determine the LC50 value. Results: The combination extract of roselle flowers (Hibiscus sabdariffa L.) and bitter melon fruit (Momordica charantia) had an LC50 value of 241.8 ppm. Keywords: BSLT, Hibiscus sabdariffa L, Momordica charantia
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