使用与人免疫球蛋白 G 非共价官能化的多壁碳纳米管修饰的玻璃碳电极进行无标记电化学传感

IF 3.7 3区 工程技术 Q2 CHEMISTRY, ANALYTICAL
M. L. Mujica, Alejandro Tamborelli, Pablo Dalmasso, Gustavo Rivas
{"title":"使用与人免疫球蛋白 G 非共价官能化的多壁碳纳米管修饰的玻璃碳电极进行无标记电化学传感","authors":"M. L. Mujica, Alejandro Tamborelli, Pablo Dalmasso, Gustavo Rivas","doi":"10.3390/chemosensors12010004","DOIUrl":null,"url":null,"abstract":"This work reports new analytical applications of glassy carbon electrodes (GCE) modified with a nanohybrid obtained by non-covalent functionalization of multi-walled carbon nanotubes (MWCNTs) with human immunoglobulin G (IgG) (GCE/MWCNT-IgG). We report the label-free and non-amplified breast cancer 1 gen (BRCA1) biosensing based on the facilitated adsorption of the DNA probe at the nanohybrid modified GCE and the impedimetric detection of the hybridization event in the presence of the redox marker benzoquinone/hydroquinone. The resulting genosensor made the fast, highly selective, and sensitive quantification of BRCA1 gene possible, with a linear range between 1.0 fM and 10.0 nM, a sensitivity of (3.0 ± 0.1) × 102 Ω M−1 (R2 = 0.9990), a detection limit of 0.3 fM, and excellent discrimination of fully non-complementary and mismatch DNA sequences. The detection of BRCA1 in enriched samples of diluted human blood serum showed a recovery percentage of 94.6%. Another interesting analytical application of MWCNT-IgG-modified GCE based on the catalytic activity of the exfoliated MWCNTs is also reported for the simultaneous quantification of dopamine and uric acid in the presence of ascorbic acid, with detection limits at submicromolar levels for both compounds.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"358 22","pages":""},"PeriodicalIF":3.7000,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Label-Free Electrochemical Sensing Using Glassy Carbon Electrodes Modified with Multiwalled-Carbon Nanotubes Non-Covalently Functionalized with Human Immunoglobulin G\",\"authors\":\"M. L. Mujica, Alejandro Tamborelli, Pablo Dalmasso, Gustavo Rivas\",\"doi\":\"10.3390/chemosensors12010004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This work reports new analytical applications of glassy carbon electrodes (GCE) modified with a nanohybrid obtained by non-covalent functionalization of multi-walled carbon nanotubes (MWCNTs) with human immunoglobulin G (IgG) (GCE/MWCNT-IgG). We report the label-free and non-amplified breast cancer 1 gen (BRCA1) biosensing based on the facilitated adsorption of the DNA probe at the nanohybrid modified GCE and the impedimetric detection of the hybridization event in the presence of the redox marker benzoquinone/hydroquinone. The resulting genosensor made the fast, highly selective, and sensitive quantification of BRCA1 gene possible, with a linear range between 1.0 fM and 10.0 nM, a sensitivity of (3.0 ± 0.1) × 102 Ω M−1 (R2 = 0.9990), a detection limit of 0.3 fM, and excellent discrimination of fully non-complementary and mismatch DNA sequences. The detection of BRCA1 in enriched samples of diluted human blood serum showed a recovery percentage of 94.6%. Another interesting analytical application of MWCNT-IgG-modified GCE based on the catalytic activity of the exfoliated MWCNTs is also reported for the simultaneous quantification of dopamine and uric acid in the presence of ascorbic acid, with detection limits at submicromolar levels for both compounds.\",\"PeriodicalId\":10057,\"journal\":{\"name\":\"Chemosensors\",\"volume\":\"358 22\",\"pages\":\"\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2023-12-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemosensors\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.3390/chemosensors12010004\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemosensors","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.3390/chemosensors12010004","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

摘要

本研究报告了用多壁碳纳米管(MWCNTs)与人免疫球蛋白 G(IgG)非共价官能化获得的纳米杂化物修饰的玻璃碳电极(GCE)(GCE/MWCNT-IgG)的新分析应用。我们报告了无标记、无扩增的乳腺癌 1 基因(BRCA1)生物传感技术,该技术基于 DNA 探针在纳米杂化修饰的 GCE 上的吸附,以及在氧化还原标记苯醌/对苯二酚存在下对杂交事件的阻抗检测。由此产生的基因传感器实现了对 BRCA1 基因的快速、高选择性和高灵敏度定量,其线性范围为 1.0 fM 至 10.0 nM,灵敏度为 (3.0 ± 0.1) × 102 Ω M-1(R2 = 0.9990),检测限为 0.3 fM,并能很好地区分完全不互补和错配的 DNA 序列。在稀释的人体血清富集样本中检测 BRCA1 的回收率为 94.6%。另一项有趣的分析应用是基于剥离的 MWCNT 催化活性的 MWCNT-IgG 改性 GCE,在抗坏血酸存在下可同时定量检测多巴胺和尿酸,两种化合物的检测限均为亚摩尔级。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Label-Free Electrochemical Sensing Using Glassy Carbon Electrodes Modified with Multiwalled-Carbon Nanotubes Non-Covalently Functionalized with Human Immunoglobulin G
This work reports new analytical applications of glassy carbon electrodes (GCE) modified with a nanohybrid obtained by non-covalent functionalization of multi-walled carbon nanotubes (MWCNTs) with human immunoglobulin G (IgG) (GCE/MWCNT-IgG). We report the label-free and non-amplified breast cancer 1 gen (BRCA1) biosensing based on the facilitated adsorption of the DNA probe at the nanohybrid modified GCE and the impedimetric detection of the hybridization event in the presence of the redox marker benzoquinone/hydroquinone. The resulting genosensor made the fast, highly selective, and sensitive quantification of BRCA1 gene possible, with a linear range between 1.0 fM and 10.0 nM, a sensitivity of (3.0 ± 0.1) × 102 Ω M−1 (R2 = 0.9990), a detection limit of 0.3 fM, and excellent discrimination of fully non-complementary and mismatch DNA sequences. The detection of BRCA1 in enriched samples of diluted human blood serum showed a recovery percentage of 94.6%. Another interesting analytical application of MWCNT-IgG-modified GCE based on the catalytic activity of the exfoliated MWCNTs is also reported for the simultaneous quantification of dopamine and uric acid in the presence of ascorbic acid, with detection limits at submicromolar levels for both compounds.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Chemosensors
Chemosensors Chemistry-Analytical Chemistry
CiteScore
5.00
自引率
9.50%
发文量
450
审稿时长
11 weeks
期刊介绍: Chemosensors (ISSN 2227-9040; CODEN: CHEMO9) is an international, scientific, open access journal on the science and technology of chemical sensors published quarterly online by MDPI.The journal is indexed in Scopus, SCIE (Web of Science), CAPlus / SciFinder, Inspec, Engineering Village and other databases.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信