Protective effect of Gundelia tournefortii extract on spleen tissue in experimental type I diabetes in rats

Aim: In this study, it was aimed to investigate the effects of Gundelia tournefortii plant extract on the spleen of rats with experimental type of rats. Methods: In the study, 35 adult Wistar Albino rats were used. Thirty-five male rats were randomly divided into five equal groups of seven rats each. Animal groups were determined as Control, Diabetes, Diabetes + Treatment 1, Diabetes + Treatment 2, Diabetes + Treatment 3. The control group was not exposed to any treatment. The other groups received 45 mg/kg Streptozotocin intraperitoneally on the experimental day. Animals in Diabetes + Treatment 1, Diabetes + Treatment 2 and Diabetes + Treatment 3 groups were administered 50 mg/kg, 100 mg/kg and 200 mg/kg of Gundelia tournefortii extract daily by oral gavage for 21 days, respectively. At the end of the experiment, the animals were euthanized, and the spleen was properly removed. The tissues were stained with hematoxylin and eosin after routine tissue tracing. Results: Diabetes mellitus caused a decrease in the diameter of the white pulp and severe atrophy in the spleen tissue. In the red pulp, on the contrary, there was an increase in activation. In the treatment groups, it was determined that Gundelia tournefortii extract minimized the damage of diabetes and even the group with images close to the control group was Diabetes + Treatment 3, while in the other treatment groups, the rate of damage repair decreased as the dose decreased. Conclusion: In the study, it was determined that diabetes caused damage to the spleen tissue and these damages were reduced by Gundelia tournefortii plant extract. In addition, in the comparison between different doses of this plant extract in the treatment groups, it was determined that the best result was obtained in the group administered at the highest dose of 200 mg/kg.    How to cite this article: Demircioğlu M, Dörtbudak MB. Protective effect of Gundelia Tournefortii extract on spleen tissue in experimental type I diabetes in rats. J Med Dent Invest 2023; 4: e230339. https://doi.org/10.5577/jomdi.e230339