纳米比亚 Dolichos Lablab(Lablab purpureus (L.) Sweet)种质的微卫星(SSR)分析

F. F. Kavishe, Percy Chimwamurombe, Jeya Kennedy, J. Uzabakiriho
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摘要

Dolichos lablab(Lablab purpureus (L.) Sweet))是一种原产于非洲的多用途耐旱、富含蛋白质的豆科作物,因其可食用的种子和肥料而种植于暖温带至热带气候地区。文献资料表明,Lablab purpureus 对自给自足的农民有很大益处,并为可持续作物生产提供了巨大前景,尤其是在边缘化地区。其用途广泛,从作为蔬菜供人食用,到提高土壤肥力和用作饲料。在纳米比亚,虽然兰花具有重要的潜在功能,但目前有关该植物遗传多样性的信息却很有限。本研究采用描述性定量研究方法,利用收集到的数据来描述所采集的样本。研究的目的是评估 26 个兰香草品种的遗传多样性。通过对三个 SSR 标记(即 VM38、AGB8 和 GATS911)进行 PCR 扩增,确定了植物的遗传多样性。这些 SSR 标记的 PIC 值分别为:标记 VM38 为 0.452663,标记 AGB8 为 0.473373,标记 GATS911 为 0.260355。香农多样性指数的值为 1.25,这意味着加入物之间的遗传多样性水平较低。该研究是纳米比亚首次报告 Lablab purpureus 的遗传多样性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microsatellite (SSR) Analysis of Dolichos Lablab (Lablab purpureus (L.) Sweet) Germplasm in Namibia
Dolichos lablab (Lablab purpureus (L.) Sweet)) is a multipurpose drought tolerant protein-rich legume crop native to Africa and grown in warm temperate to tropical climates for its edible seeds and manure. Literature informs that Lablab purpureus holds significant benefits to subsistence farmers and offers a great promise for sustainable crop productivity, especially in marginalised areas. Its uses range from human consumption as a vegetable to improving soil fertility, and as forage. Not with standing Lablab purpureus crucial potential functions in Namibia, there is currently limited information regarding the plant’s genetic diversity. This study followed a descriptive quantitative research approach where the data collected was used to describe the samples collected. The study aimed at evaluating the genetic diversity among 26 accessions of Lablab purpureus. The plant’s genetic diversity was determined by PCR amplification of three SSR markers namely VM38, AGB8 and GATS911. The SSR markers had PIC values of 0.452663 for marker VM38, 0.473373 for marker AGB8 and 0.260355 for marker GATS911. The Shannon Index of diversity gave a value of 1.25 meaning low levels of genetic diversity among the accessions. The study was the first report of Lablab purpureus’s genetic diversity in Namibia.
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