利用 ABTS 法评估小檗果实的抗氧化活性

Anna Och, Agata Ćwikła, Karolina Orzechowska, A. Cwener
{"title":"利用 ABTS 法评估小檗果实的抗氧化活性","authors":"Anna Och, Agata Ćwikła, Karolina Orzechowska, A. Cwener","doi":"10.15584/ejcem.2023.4.31","DOIUrl":null,"url":null,"abstract":"Introduction and aim. The assessment of the antioxidant activity of plant extracts is an important research direction in the search for new chemopreventive substances. The aim of this study was to demonstrate the antioxidant potential of the methanol extract of Berberis jaeschkeana C. K. Schneid fruit. Material and methods. Spectrophotometric tests were carried out: testing of the total content of polyphenols (TPC) in extracts, testing of the total content of flavonoids (TFC) in extracts. Antiradical activity was determined using the improved ABTS+• decolorization test with modifications. Results. TPC extracts was determined at the level of 43.52±2.37 mg/g, while TFC extracts was determined at the level 6.08±0.48 mg/g. The ABTS activity test showed 31.15 mg/g expressed as mg Trolox per g of dry extract. Conclusion. It was assessed that the methanolic extract of the fruit of Berberis jaeschkeana C. K. Schneid. has an antioxidant properties, what makes it a potential chemopreventive agent in civilizational diseases.","PeriodicalId":11828,"journal":{"name":"European Journal of Clinical and Experimental Medicine","volume":" 42","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of the antioxidant activity of Berberis jaeschkeana C. K. Schneid. fruits using the ABTS assay\",\"authors\":\"Anna Och, Agata Ćwikła, Karolina Orzechowska, A. Cwener\",\"doi\":\"10.15584/ejcem.2023.4.31\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Introduction and aim. The assessment of the antioxidant activity of plant extracts is an important research direction in the search for new chemopreventive substances. The aim of this study was to demonstrate the antioxidant potential of the methanol extract of Berberis jaeschkeana C. K. Schneid fruit. Material and methods. Spectrophotometric tests were carried out: testing of the total content of polyphenols (TPC) in extracts, testing of the total content of flavonoids (TFC) in extracts. Antiradical activity was determined using the improved ABTS+• decolorization test with modifications. Results. TPC extracts was determined at the level of 43.52±2.37 mg/g, while TFC extracts was determined at the level 6.08±0.48 mg/g. The ABTS activity test showed 31.15 mg/g expressed as mg Trolox per g of dry extract. Conclusion. It was assessed that the methanolic extract of the fruit of Berberis jaeschkeana C. K. Schneid. has an antioxidant properties, what makes it a potential chemopreventive agent in civilizational diseases.\",\"PeriodicalId\":11828,\"journal\":{\"name\":\"European Journal of Clinical and Experimental Medicine\",\"volume\":\" 42\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-12-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"European Journal of Clinical and Experimental Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15584/ejcem.2023.4.31\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Clinical and Experimental Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15584/ejcem.2023.4.31","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

引言和目的。评估植物提取物的抗氧化活性是寻找新的化学预防物质的一个重要研究方向。本研究旨在证明小檗(Berberis jaeschkeana C. K. Schneid)果实甲醇提取物的抗氧化潜力。材料和方法。进行了分光光度测试:测试提取物中多酚的总含量(TPC),测试提取物中黄酮类化合物的总含量(TFC)。使用改进的 ABTS+- 脱色试验测定抗自由基活性。结果TPC 提取物的含量为 43.52±2.37 mg/g,TFC 提取物的含量为 6.08±0.48 mg/g。ABTS 活性测试显示为 31.15 mg/g,以每克干提取物含 mg Trolox 表示。结论经评估,红叶小檗果实的甲醇提取物具有抗氧化特性,使其成为潜在的文明病化学预防剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of the antioxidant activity of Berberis jaeschkeana C. K. Schneid. fruits using the ABTS assay
Introduction and aim. The assessment of the antioxidant activity of plant extracts is an important research direction in the search for new chemopreventive substances. The aim of this study was to demonstrate the antioxidant potential of the methanol extract of Berberis jaeschkeana C. K. Schneid fruit. Material and methods. Spectrophotometric tests were carried out: testing of the total content of polyphenols (TPC) in extracts, testing of the total content of flavonoids (TFC) in extracts. Antiradical activity was determined using the improved ABTS+• decolorization test with modifications. Results. TPC extracts was determined at the level of 43.52±2.37 mg/g, while TFC extracts was determined at the level 6.08±0.48 mg/g. The ABTS activity test showed 31.15 mg/g expressed as mg Trolox per g of dry extract. Conclusion. It was assessed that the methanolic extract of the fruit of Berberis jaeschkeana C. K. Schneid. has an antioxidant properties, what makes it a potential chemopreventive agent in civilizational diseases.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
0.20
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信